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抗人CD8a抗体，克隆RPA-T8

小鼠单克隆IgG1抗体，抗人、恒河猴、食蟹猴CD8a
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产品号 #60022_C

小鼠单克隆IgG1抗体，抗人、恒河猴、食蟹猴CD8a

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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

RPA-T8抗体识别CD8a，CD8a是一种32 - 34 kDa的I型跨膜糖蛋白，是CD8的一个亚基。CD8是一种二硫键结合的二聚体，可以是CD8a （α）和CD8b （β）亚基的异二聚体（即αβ）或同二聚体（αα）。CD8通过结合抗原呈递细胞呈递的MHC I类分子，在T细胞活化过程中作为T细胞受体（TCR）的共受体。其功能是加强TCR与MHC I-抗原复合物之间的联系，并通过其胞内结构域与细胞质酪氨酸激酶（如Lck）的相互作用，放大从TCR到细胞质的信号。CD8a链与MHC I类分子的α3结构域结合。CD8在大多数胸腺细胞、一部分成熟的外周血T细胞（细胞毒性T细胞）、和一部分β T细胞上表达，在NK细胞上也有较低水平的表达（主要表达CD8a同二聚体）。

该抗体克隆已被验证可用于评估EasySep™试剂盒（包括EasySep™Direct人CD8+ T细胞分选试剂盒（产品号 #19663）、EasySep™人CD8+ T细胞富集试剂盒（产品号 #19053）、EasySep™人CD8正选试剂盒（产品号 #18053）、EasySep™人CD3正选试剂盒（产品号 #18051）、EasySep™HLA CD2正选试剂盒（产品号 #18657HLA）和EasySep™HLA T细胞富集试剂盒（产品号 #19051HLA））分选的细胞纯度。

亚型
一抗
 
靶抗原
CD8a
 
别名
Leu2，T8
 
活性物种
Baboon，Chimpanzee，Cynomolgus，人，Macaque，Rhesus，Sooty Mangabey
 
偶联
APC，Biotin 或 生物素，FITC，PE，PerCP-Cyanine5.5，未偶联的
 
宿主物种
小鼠
 
细胞类型
T 细胞，T 细胞，CD8+
 
种属
人，非人灵长类
 
应用
细胞分选，CyTOF，流式细胞术，功能学筛选，免疫组化
 
研究领域
免疫
 
克隆
RPA-T8
 
基因编号
925
 
同种型
IgG1，kappa
 

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Data Figures

Data for APC-Conjugated

Figure 1. Data for APC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD8a Antibody, Clone RPA-T8, APC and Anti-Human CD3 Antibody, Clone UCHT1, FITC (Catalog #60011FI).
(B) Flow cytometry analysis of human PBMCs labeled with Anti-Human CD8a Antibody, Clone RPA-T8, APC and Anti-Human CD45 Antibody, Clone HI30, FITC (Catalog #60018FI).
(C) Flow cytometry analysis of human PBMCs labeled with Anti-Human CD8a Antibody, Clone RPA-T8, APC and a mouse IgG1, kappa FITC isotype control antibody.
(D) Flow cytometry analysis of human PBMCs processed with the EasySep™ HLA T Cell Enrichment Kit and labeled with Anti-Human CD8a Antibody, Clone RPA-T8, APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (open histogram).

Data for Biotin-Conjugated

Figure 2. Data for Biotin-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD8a Antibody, Clone RPA-T8, Biotin followed by streptavidin (SAV) APC (filled histogram) or a mouse IgG1, kappa biotin isotype control antibody followed by SAV APC (black line histogram).

Data for FITC-Conjugated

Figure 3. Data for FITC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD8a Antibody, Clone RPA-T8, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD3 Positive Selection Kit and labeled with Anti-Human CD8a Antibody, Clone RPA-T8, FITC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (solid line histogram).

Data for PE-Conjugated

Figure 4. Data for PE-Conjugated

(A) Flow cytometry analysis of peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD8a Antibody, Clone RPA-T8, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ HLA T Cell Enrichment Kit and labeled with Anti-Human CD8a Antibody, Clone RPA-T8, PE. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa PE isotype control antibody is shown (solid line histogram).

Data for Unconjugated

Figure 5. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD8a Antibody, Clone RPA-T8, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 6. Data for PerCP-Cy55-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD8a Antibody, Clone RPA-T8, PerCP-Cy5.5 (filled histogram) or a mouse IgG1, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram). (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD8 Positive Selection Kit and labeled with Anti-Human CD8a Antibody, Clone RPA-T8, PerCP-Cy5.5. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa PerCP-Cy5.5 isotype control antibody is shown (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD8a Antibody, Clone RPA-T8, APC
Catalog #
60022AZ.1, 60022AZ
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD8a Antibody, Clone RPA-T8, FITC
Catalog #
60022FI, 60022FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD8a Antibody, Clone RPA-T8, PE
Catalog #
60022PE, 60022PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD8a Antibody, Clone RPA-T8, PerCP-Cy5.5
Catalog #
60022PS
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD8a Antibody, Clone RPA-T8
Catalog #
60022
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD8a Antibody, Clone RPA-T8, APC
Catalog #
60022AZ.1, 60022AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD8a Antibody, Clone RPA-T8, FITC
Catalog #
60022FI, 60022FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD8a Antibody, Clone RPA-T8, PE
Catalog #
60022PE, 60022PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD8a Antibody, Clone RPA-T8, PerCP-Cy5.5
Catalog #
60022PS
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD8a Antibody, Clone RPA-T8
Catalog #
60022
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (3)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

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更多信息
Species Human, Non-Human Primate
Clone RPA-T8
Gene Id 925
Alternative Names Leu2, T8
Isotype IgG1, kappa
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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