产品号 #70024_C
冻存的人原代细胞
Save on high-quality cells! Take advantage of our bulk pricing discounts and price matching offer to get the best value for your purchase. Browse our Frequently Asked Questions for more information.
总览
选择即用型、符合伦理的人原代Pan T细胞。我们提供个性化的服务、定制产品、灵活的交付周期，并支持提前测试筛选并预留整个批次，以确保您获得所需的细胞。
通过免疫磁珠负选从外周血单个核细胞中分离细胞，并冻存于不含动物成分的CryoStor® CS10冻存液（产品号#07930）中，Pan T细胞包括CD4和CD8 T细胞以及一些γ/δ T细胞亚群。遵循机构审查委员会（IRB）批准的知情同意书和方案进行收集。如有需要，可提供其他文档、高分辨率HLA分型（I类和II类等位基因）以及CMV状态。采集过程中添加酸-柠檬酸-葡萄糖溶液A （ACDA）作为抗凝剂。选择产品选项后，供体信息（如BMI范围、吸烟状态、种族等）可以在上面的评论框中查询。所有供体均经过HIV-1/2、乙肝和丙肝筛查。
某些产品仅在特定地区出售。请与您当地的销售代表或产品与科学支持联系techsupport@stemcell.com获取更多信息。
欲了解更多信息，请浏览有关原代细胞的常见问题解答（FAQs）。
包含
CryoStor® CS10
亚型
冻存
细胞类型
T 细胞
种属
人
细胞和组织来源
外周血
供体状态
Normal
纯度
流式细胞术检测：CD3+ ≥ 85%
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (8)
Publications (3)
Paediatric patients with acute leukaemia and KMT2A (MLL) rearrangement show a distinctive expression pattern of histone deacetylases. British journal of haematology 2018 AUG
Abstract
Histone deacetylase inhibitors (HDACi) had emerged as promising drugs in leukaemia, but their toxicity due to lack of specificity limited their use. Therefore, there is a need to elucidate the role of HDACs in specific settings. The study of HDAC expression in childhood leukaemia could help to choose more specific HDACi for selected candidates in a personalized approach. We analysed HDAC1-11, SIRT1, SIRT7, MEF2C and MEF2D mRNA expression in 211 paediatric patients diagnosed with acute leukaemia. There was a global overexpression of HDACs, while specific HDACs correlated with clinical and biological features, and some even predicted outcome. Thus, some HDAC and MEF2C profiles probably reflected the lineage and the maturation of the blasts and some profiles identified specific oncogenic pathways active in the leukaemic cells. Specifically, we identified a distinctive signature for patients with KMT2A (MLL) rearrangement, with high HDAC9 and MEF2D expression, regardless of age, KMT2A partner and lineage. Moreover, we observed an adverse prognostic value of HDAC9 overexpression, regardless of KMT2A rearrangement. Our results provide useful knowledge on the complex picture of HDAC expression in childhood leukaemia and support the directed use of specific HDACi to selected paediatric patients with acute leukaemia.
Human dendritic cells derived from embryonic stem cells stably modified with CD1d efficiently stimulate antitumor invariant natural killer T cell response. Stem cells translational medicine 2014 JAN
Abstract
Invariant natural killer T (iNKT) cells are a unique lymphocyte subpopulation that mediates antitumor activities upon activation. A current strategy to harness iNKT cells for cancer treatment is endogenous iNKT cell activation using patient-derived dendritic cells (DCs). However, the limited number and functional defects of patient DCs are still the major challenges for this therapeutic approach. In this study, we investigated whether human embryonic stem cells (hESCs) with an ectopically expressed CD1d gene could be exploited to address this issue. Using a lentivector carrying an optimized expression cassette, we generated stably modified hESC lines that consistently overexpressed CD1d. These modified hESC lines were able to differentiate into DCs as efficiently as the parental line. Most importantly, more than 50% of such derived DCs were CD1d+. These CD1d-overexpressing DCs were more efficient in inducing iNKT cell response than those without modification, and their ability was comparable to that of DCs generated from monocytes of healthy donors. The iNKT cells expanded by the CD1d-overexpressing DCs were functional, as demonstrated by their ability to lyse iNKT cell-sensitive glioma cells. Therefore, hESCs stably modified with the CD1d gene may serve as a convenient, unlimited, and competent DC source for iNKT cell-based cancer immunotherapy.
Enhancing Immunostimulatory Function of Human Embryonic Stem Cell-Derived Dendritic Cells by CD1d Overexpression The Journal of Immunology 2012 MAY
Abstract
Human embryonic stem cell-derived dendritic cells (hESC-DCs) may potentially provide a platform to generate off-the-shelf" therapeutic cancer vaccines. To apply hESC-DCs for cancer immunotherapy in a semiallogeneic setting�
更多信息
| Species | Human |
|---|---|
| Contains | CryoStor® CS10 |
| Purity | ≥ 85% CD3+ by flow cytometry |
| Cell And Tissue Source | Peripheral Blood |
| Donor Status | Normal |
相关产品
-
ThawSTAR® CFT2全自动细胞解冻系统全自动细胞解冻系统，确保一致的解冻性能
-
EasySep™人T细胞富集试剂盒通过免疫磁珠负选分离出无磁珠标记的人T细胞
-
抗人CD4抗体，克隆OKT4小鼠单克隆IgG2b抗体，抗人、恒河猴、食蟹猴CD4
-
冻存的人外周血CD4+ T细胞冻存的人原代细胞
-
冻存的人外周血CD8+ T细胞冻存的人原代细胞
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
