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ThawSTAR® CFT2转运盒

用于处理和运输来自液氮或-80°C 冷冻柜的冻存管的便携式解决方案
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产品号 #100-0642_C

用于处理和运输来自液氮或-80°C 冷冻柜的冻存管的便携式解决方案

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总览

保护细胞免受瞬时升温事件的影响，维持最大限度的细胞健康状态；在冻存管运输过程中使用ThawSTAR® CFT2转运盒。

该转运盒是一种便携式解决方案，用于处理和运输冻存管，从在气相液氮或 -80°C 冰箱中的长期储存，到使用ThawSTAR® CFT2全自动细胞解冻系统进行下游处理。ThawSTAR® CFT2全自动细胞解冻系统提供无水标准化细胞解冻流程，配有ThawSTAR® CFT2转运盒，两个ThawSTAR® CFT2测试样品管，以及ThawSTAR® CFT2电源和适配器。对于性能鉴定文件和功能测试所需的配件，请使用ThawSTAR® CFT2 IOPQ试剂盒（可单独购买）。

有关使用ThawSTAR®进行细胞解冻方案的更多信息，请浏览技术手册和教育材料."

应用
冻存
 
品牌
ThawSTAR
 
研究领域
脐带血库，免疫
 

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Data Figures

Bar graphs comparing the number of live cells recovered and percentage viability for peripheral blood mononuclear cells (PBMCs) thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 1. Frozen PBMCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Peripheral blood mononuclear cells (PBMCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 3 x 107 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.78 x 107 vs. 2.96 x 107 cells, respectively and (B) the mean viability was 92.8% vs. 93.9%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Bar graphs comparing the number of live cells recovered and percentage viability for monocytes thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 2. Frozen Monocytes Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Monocytes cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 2 x 107 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.05 x 107 vs. 1.89 x 107 cells, respectively and (B) the mean viability was 95% vs. 94.6%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Bar graphs comparing the number of live cells recovered and percentage viability for human pluripotent stem cells (hPSCs) thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 3. Frozen hPSCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Human pluripotent stem cells (hPSCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 1 x 106 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 9.05 x 105 vs. 9.35 x 105 cells, respectively and (B) the mean viability was 83.04% vs. 82.93%, respectively. The hPSCs were from 3 different cell lines (M001, 1C, and H9) and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
ThawSTAR® CFT2 Transporter
Catalog #
100-0642
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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Workflow Stages
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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