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ThawSTAR® CFT2测试样品管

用于每次解冻之前和之后记录仪器性能和审计跟踪
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产品号 #100-0643_C

用于每次解冻之前和之后记录仪器性能和审计跟踪

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总览

使用ThawSTAR® CFT2测试样品管记录ThawSTAR® CFT2全自动细胞解冻系统的性能。在与待解冻生物样本相同的条件下使用 ThawSTAR® CFT2测试样品管，评估并记录每次解冻前后的解冻性能，并创建审计追踪。为了方便起见，ThawSTAR® CFT2测试样品管可单独购买，也可以作为ThawSTAR® CFT2全自动细胞解冻系统的一部分购买。ThawSTAR® CFT2全自动细胞解冻系统配有两个ThawSTAR® CFT2测试样品管，ThawSTAR® CFT2转运盒，ThawSTAR® CFT2电源和适配器，可实现无水标准化细胞解冻流程。如需获取性能鉴定文件和功能测试所需的配件，请使用ThawSTAR® CFT2 IOPQ试剂盒（可单独购买）。

有关使用ThawSTAR®进行细胞解冻方案的更多信息，请浏览技术手册.

应用
冻存
 
品牌
ThawSTAR
 
研究领域
脐带血库，免疫
 

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Data Figures

Bar graphs comparing the number of live cells recovered and percentage viability for peripheral blood mononuclear cells (PBMCs) thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 1. Frozen PBMCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Peripheral blood mononuclear cells (PBMCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 3 x 107 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.78 x 107 vs. 2.96 x 107 cells, respectively and (B) the mean viability was 92.8% vs. 93.9%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Bar graphs comparing the number of live cells recovered and percentage viability for monocytes thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 2. Frozen Monocytes Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Monocytes cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 2 x 107 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.05 x 107 vs. 1.89 x 107 cells, respectively and (B) the mean viability was 95% vs. 94.6%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Bar graphs comparing the number of live cells recovered and percentage viability for human pluripotent stem cells (hPSCs) thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 3. Frozen hPSCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Human pluripotent stem cells (hPSCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 1 x 106 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 9.05 x 105 vs. 9.35 x 105 cells, respectively and (B) the mean viability was 83.04% vs. 82.93%, respectively. The hPSCs were from 3 different cell lines (M001, 1C, and H9) and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Safety Data Sheet
Product Name
ThawSTAR® CFT2 Confirmation Vials
Catalog #
100-0643
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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