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ThawSTAR® CFT2 IOPQ试剂盒

包含安装、操作和性能验证文档及配件，以方便对ThawSTAR® CFT2进行功能测试
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产品号 #100-0730_C

包含安装、操作和性能验证文档及配件，以方便对ThawSTAR® CFT2进行功能测试

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ThawSTAR® CFT2 IOPQ试剂盒包含安装、操作和性能验证文档以及测试工具配件，方便对ThawSTAR® CFT2全自动细胞解冻系统进行功能测试。ThawSTAR® CFT2全自动细胞解冻系统提供无水标准化细胞解冻流程，配备ThawSTAR® CFT2转运盒、两个ThawSTAR® CFT2测试样品管以及ThawSTAR® CFT2电源和适配器。

使用ThawSTAR® CFT2 IOPQ试剂盒，用户可以获得基于GxP行业标准和GAMP 5方案的分步IOPQ方案和摘要报告模板。

如需了解更多关于使用ThawSTAR®进行细胞解冻方案的信息，请参阅技术手册.

应用
冻存
 
品牌
ThawSTAR
 
研究领域
脐带血库，免疫
 

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Data Figures

Bar graphs comparing the number of live cells recovered and percentage viability for peripheral blood mononuclear cells (PBMCs) thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 1. Frozen PBMCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Peripheral blood mononuclear cells (PBMCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 3 x 107 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.78 x 107 vs. 2.96 x 107 cells, respectively and (B) the mean viability was 92.8% vs. 93.9%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Bar graphs comparing the number of live cells recovered and percentage viability for monocytes thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 2. Frozen Monocytes Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Monocytes cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 2 x 107 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.05 x 107 vs. 1.89 x 107 cells, respectively and (B) the mean viability was 95% vs. 94.6%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Bar graphs comparing the number of live cells recovered and percentage viability for human pluripotent stem cells (hPSCs) thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 3. Frozen hPSCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Human pluripotent stem cells (hPSCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 1 x 106 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 9.05 x 105 vs. 9.35 x 105 cells, respectively and (B) the mean viability was 83.04% vs. 82.93%, respectively. The hPSCs were from 3 different cell lines (M001, 1C, and H9) and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Safety Data Sheet
Product Name
ThawSTAR® CFT2 IOPQ Kit
Catalog #
100-0730
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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Workflow Stages
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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