The ThawSTAR® CFT2 IOPQ Kit includes installation, operational, and performance qualification documentation and testing tool accessories to facilitate functional testing of the ThawSTAR® CFT2 Automated Thawing System. Providing a water-free and standardized cell thawing process, the ThawSTAR® CFT2 Automated Thawing System comes complete with a ThawSTAR® CFT2 Transporter, two ThawSTAR® CFT2 Confirmation Vials, and ThawSTAR® CFT2 Power Supply and Adaptors.
Use the ThawSTAR® CFT2 IOPQ Kit for a step-by-step IOPQ protocol and summary report templates that are based upon GxP industry standards and the GAMP 5 methodology.
For more information on protocols for cell thawing using ThawSTAR®, please explore the Technical Manual.
Use the ThawSTAR® CFT2 IOPQ Kit for a step-by-step IOPQ protocol and summary report templates that are based upon GxP industry standards and the GAMP 5 methodology.
For more information on protocols for cell thawing using ThawSTAR®, please explore the Technical Manual.
Data Figures
Figure 1. Frozen PBMCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability
Peripheral blood mononuclear cells (PBMCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 3 x 107 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.78 x 107 vs. 2.96 x 107 cells, respectively and (B) the mean viability was 92.8% vs. 93.9%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.
Figure 2. Frozen Monocytes Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability
Monocytes cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 2 x 107 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.05 x 107 vs. 1.89 x 107 cells, respectively and (B) the mean viability was 95% vs. 94.6%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.
Figure 3. Frozen hPSCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability
Human pluripotent stem cells (hPSCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 1 x 106 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 9.05 x 105 vs. 9.35 x 105 cells, respectively and (B) the mean viability was 83.04% vs. 82.93%, respectively. The hPSCs were from 3 different cell lines (M001, 1C, and H9) and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Safety Data Sheet
Product Name
ThawSTAR® CFT2 IOPQ Kit
Catalog #
100-0730
Lot #
All
Language
English
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Monocyte Research
Workflow Stages for Cord Blood Banking
Resources and Publications
Educational Materials (6)
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