STEMdiff™ 三胚层分化试剂盒

功能检测试剂盒,用于评估人ES和iPS细胞定向分化至三个胚层的多能性

产品号 #(选择产品)

产品号 #05230_C

功能检测试剂盒,用于评估人ES和iPS细胞定向分化至三个胚层的多能性

产品优势

  • 可在多种多能性细胞系中实现可重复的三胚层分化
  • 检测结果易于解读
  • 完整、成分明确的培养基
  • 标准化的一周实验流程

产品组分包括

  • STEMdiff™ 三胚层外胚层培养基,175 mL
  • STEMdiff™ 三胚层中胚层培养基,100 mL
  • STEMdiff™ 三胚层内胚层培养基,100 mL

总览

STEMdiff™ 三胚层分化试剂盒提供了一种简单的培养检测方法,用于功能性地验证新建或已建立的人胚胎干细胞 (ES) 和诱导多能干细胞 (iPS) 系分化为三个胚层(外胚层、中胚层和内胚层)的能力。该试剂盒包含专门的完整培养基和基于单层细胞的实验方案,可对每个胚层进行平行的体外定向分化实验,并在一周内清晰且可重复地确认三胚层分化潜能。STEMdiff™ 三胚层分化试剂盒设计为终点检测用途,不适用于后续分化或其他应用所需细胞的制备。STEMdiff™ 三胚层分化试剂盒设计为终点检测用途,不适用于后续分化或其他应用所需细胞的制备。本试剂盒已针对在 mTeSR™1、mTeSR™ Plus 或 TeSR™-AOF 培养的细胞进行了优化。

亚型
专用培养基
 
细胞类型
多能干细胞
 
种属

 
应用
细胞培养,鉴定,分化,功能学筛选,表型鉴定
 
品牌
STEMdiff
 
研究领域
干细胞生物学
 

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
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Language
Catalog #
05230
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All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
05230
Lot #
All
Language
English
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Safety Data Sheet 2
Catalog #
05230
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
05230
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (32)

Publications (3)

Feeder-Free Derivation of Naïve Human Pluripotent Stem Cells. Ward E et al. Stem cells and development 2017 MAY

Abstract

Human pluripotent stem cells (HPSCs) cultured in conditions that maintain pluripotency via FGF and TGFβ signaling have been described as being in a primed state. These cells have been shown to exhibit characteristics more closely related to mouse epiblast-derived stem cells than to so called naïve mouse PSCs said to possess a more ground state pluripotency that mimics the early mouse embryo inner cell mass. Initial attempts to create culture conditions favorable for generation of naïve HPSCs from primed HPSCs has required the use of mouse embryonic fibroblasts as a feeder layer to support this transition. A protocol for the routine derivation and maintenance of naïve HPSCs in completely defined conditions is highly desirable for stem cell researchers to enhance the study and clinical translation of naïve HPSCs. Here we describe a standard protocol for transitioning primed HPSCs to a naïve state using commercial RSet media and xeno-free recombinant vitronectin.
Peripheral blood derived induced pluripotent stem cells (iPSCs) from a female with familial hypertrophic cardiomyopathy. S. B. Ross et al. Stem cell research 2017

Abstract

Induced pluripotent stem cells (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) obtained from a 62-year-old female with familial hypertrophic cardiomyopathy (HCM). PBMCs were reprogrammed to a pluripotent state following transfection with non-integrative episomal vectors carrying reprogramming factors OCT4, SOX2, LIN28, KLF4 and L-MYC. iPSCs were shown to express pluripotency markers, possess trilineage differentiation potential, carry rare variants identified in DNA isolated directly from the patient's whole blood, have a normal karyotype and no longer carry episomal vectors for reprogramming. This line is a useful resource for identifying unknown genetic causes of HCM.
Generation of induced pluripotent stem cells (iPSCs) from a hypertrophic cardiomyopathy patient with the pathogenic variant p.Val698Ala in beta-myosin heavy chain (MYH7) gene. S. B. Ross et al. Stem cell research 2017

Abstract

Induced pluripotent stem cells (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) isolated from the whole blood of a 43-year-old male with hypertrophic cardiomyopathy (HCM) who carries the pathogenic variant p.Val698Ala in beta-myosin heavy chain (MYH7). Patient-derived PBMCs were reprogrammed using non-integrative episomal vectors containing reprogramming factors OCT4, SOX2, LIN28, KLF4 and L-MYC. iPSCs were shown to express pluripotent markers, have trilineage differentiation potential, carry the pathogenic MYH7 variant p.Val698Ala, have a normal karyotype and no longer carry the episomal reprogramming vector. This line is useful for studying the link between variants in MYH7 and the pathogenesis of HCM.

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Species Human
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