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抗人CD184（CXCR4）抗体，克隆12G5

小鼠单克隆IgG2a抗体，抗人、恒河猴、食蟹猴CD184（CXCR4）
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产品号 #60089_C

小鼠单克隆IgG2a抗体，抗人、恒河猴、食蟹猴CD184（CXCR4）

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总览
实验数据
产品说明书及文档
应用领域
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总览

12G5 抗体可识别 CD184（又称 CXCR4 或 fusin），该蛋白是一种约 45 kDa 的七次跨膜 G 蛋白偶联受体，广泛表达于多种细胞表面，包括 T 细胞、B 细胞、树突状细胞、单核细胞、巨噬细胞、粒细胞、血小板、内皮细胞、上皮细胞、星形胶质细胞和神经元。CD184 至少有两种亚型，由可变剪接的 mRNA 转录产生。其配体包括 CXC 趋化因子 SDF-1 和泛素。CD184 在趋化反应、B 细胞淋巴生成、髓系细胞生成、心脏发育、血管生成及神经引导等过程中发挥重要功能。它还作为某些 HIV-1 T 细胞嗜性分离株感染的共受体，以及 HIV-2 在不依赖 CD4 的情况下感染细胞的替代受体，而这些功能可被 12G5 抗体结合后阻断。该抗体还可部分抑制 SDF-1 诱导的趋化作用和钙离子内流。在分化研究中，CD184 常与其他标志物（如 CD117）联合使用，以监测定型内胚层的出现。

亚型
一抗
 
靶抗原
CD184 (CXCR4)
 
别名
C-X-C 趋化因子受体4型，CXCR4，fusin，HM89，HUMSTR，LAP3，LCR1，LESTR，NPYY3R，NPY3R，SDF-1 receptor，WHIM
 
活性物种
African Green Monkey，Baboon，Chimpanzee，Cynomolgus，人，Rhesus，Sooty Mangabey
 
偶联
APC，Biotin 或 生物素，PE，未偶联的
 
宿主物种
小鼠
 
细胞类型
内胚层，PSC衍生，多能干细胞
 
种属
人，非人灵长类
 
应用
CyTOF，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化
 
研究领域
免疫，干细胞生物学
 
克隆
12G5
 
基因编号
7852
 
同种型
IgG2a，kappa
 

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Data Figures

Data for APC-Conjugated

Figure 1. Data for APC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, APC (filled histogram) or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, APC (Catalog #60071AZ) (solid line histogram).

Data for Biotin-Conjugated

Figure 2. Data for Biotin-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin (Catalog #60071BT), followed by SAV APC (solid line histogram).

Data for PE-Conjugated

Figure 3. Data for PE-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, PE (filled histogram) or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE (Catalog #60071PE) (solid line histogram). (B) Flow cytometry analysis of human embryonic stem (ES) cells following differentiation to definitive endoderm using the STEMdiff™ Definitive Endoderm Kit. Cells were labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, APC (Catalog #60087AZ) and Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, PE. The emergence of definitive endoderm can be monitored by co-expression of CD117 and CD184. (C) Flow cytometry analysis of human ES cells following differentiation to definitive endoderm using the STEMdiff™ Definitive Endoderm Kit. Cells were labeled with a mouse IgG1, kappa APC isotype control antibody and Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE.

Data for Unconjugated

Figure 4. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on lymphocytes) labeled with Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, followed by anti-mouse IgG, FITC (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173 (Catalog #60071), followed by anti-mouse IgG, FITC (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, Biotin
Catalog #
60089BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, PE
Catalog #
60089PE.1, 60089PE
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD184 (CXCR4) Antibody, Clone 12G5
Catalog #
60089
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, APC
Catalog #
60089AZ, 60089AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, Biotin
Catalog #
60089BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, PE
Catalog #
60089PE.1, 60089PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD184 (CXCR4) Antibody, Clone 12G5
Catalog #
60089
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, APC
Catalog #
60089AZ, 60089AZ.1
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (2)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

更多信息

更多信息
Species Human, Non-Human Primate
Clone 12G5
Gene Id 7852
Alternative Names C-X-C chemokine receptor type 4, CXCR4, fusin, HM89, HUMSTR, LAP3, LCR1, LESTR, NPYY3R, NPY3R, SDF-1 receptor, WHIM
Isotype IgG2a, kappa
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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