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RosetteSep™人祖细胞基础预富集试剂盒

免疫密度负选试剂混合物
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产品号 #（选择产品）

产品号 #15226_C

免疫密度负选试剂混合物

产品优势

  • 快捷、操作简单
  • 不需要特殊设备或额外培训
  • 获得的活细胞无标记
  • 可与SepMate™联合使用，实现一致发高通量样本处理

产品组分包括

  • RosetteSep™人祖细胞基础预富集抗体混合物（产品号#15226）          
    • RosetteSep™人祖细胞基础预富集抗体混合物，2mL
  • RosetteSep™人祖细胞基础预富集抗体混合物（产品号#15266）
    • RosetteSep™人祖细胞基础预富集抗体混合物，5x2mL
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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

RosetteSep™人类祖细胞基础预富集试剂盒通过阴性选择从脐带血中预富集造血祖细胞。四聚体抗体复合物可识别 CD3、CD11b、CD19以及红细胞（RBC）上的糖蛋白A，从而靶向去除非目的细胞。当在密度梯度介质如Lymphoprep™产品号#18060）上离心时，非目的细胞会与红细胞一起沉淀。预富集的祖细胞为血浆和密度梯度离心液的交界界面中高度富集的细胞。    

随后可继续使用EasySep™（例如EasySep™人类CD34正选试剂盒）从预富集的细胞群中阳性选择目标细胞。

磁体兼容性
 
 
亚型
细胞分选试剂盒
 
细胞类型
造血干/祖细胞
 
种属
人
 
样本来源
Cord Blood
 
筛选方法
Negative
 
应用
细胞分选
 
品牌
RosetteSep
 
研究领域
免疫，干细胞生物学
 

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Data Figures

Typical RosetteSep™ Human Progenitor Cell Basic Preenrichment Profile

Figure 1.Typical RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Profile

Starting with fresh human cord blood, the CD34+CD45+ viable cell content of the pre-enriched fraction typically ranges from 2 - 16%

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail
Catalog #
15266, 15226
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail
Catalog #
15266, 15226
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (3)

Human Immune Cytokines
Wallchart
Human Immune Cytokines
How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute Protocol
1:46
Video
How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute Protocol
Isolate Cells from Whole Blood without Columns or Magnets: RosetteSep™ Immunodensity Cell Separation
2:19
Video
Isolate Cells from Whole Blood without Columns or Magnets: RosetteSep™ Immunodensity Cell Separati...

Frequently Asked Questions

What is RosetteSep™?

RosetteSep™ is a rapid cell separation procedure for the isolation of purified cells directly from whole blood, without columns or magnets.

How does RosetteSep™ work?

The antibody cocktail crosslinks unwanted cells to red blood cells (RBCs), forming rosettes. The unwanted cells then pellet with the free RBCs when centrifuged over a density centrifugation medium (e.g. Ficoll-Paque™ PLUS, Lymphoprep™).

What factors affect cell recovery?

The temperature of the reagents can affect cell recovery. All reagents should be at room temperature (sample, density centrifugation medium, PBS, centrifuge) before performing the isolations. Layering can also affect recovery so be sure to carefully layer the sample to avoid mixing with the density centrifugation medium as much as possible. Be sure to collect the entire enriched culture without disturbing the RBC pellet. A small amount of density centrifugation medium can be collected without worry.

Which cell samples can RosetteSep™ be used with?

RosetteSep™ can be used with leukapheresis samples, bone marrow or buffy coat, as long as: the concentration of cells does not exceed 5 x 107 per mL (can dilute if necessary); and there are at least 100 RBCs for every nucleated cell (RBCs can be added if necessary).

Can RosetteSep™ be used with previously frozen or cultured cells?

Yes. Cells should be re-suspended at 2 - 5 x 107 cells / mL in PBS + 2% FBS. Fresh whole blood should be added at 250 µL per mL of sample, as a source of red cells.

Can RosetteSep™ be used to enrich progenitors from cord blood?

Yes. Sometimes cord blood contains immature nucleated red cells that have a lower density than mature RBCs. These immature red cells do not pellet over Ficoll™, which can lead to a higher RBC contamination than peripheral blood separations.

Does RosetteSep™ work with mouse cells?

No, but we have developed EasySep™, a magnetic-based cell isolation system which works with mouse and other non-human species.

Which anticoagulant should be used with RosetteSep™?

Peripheral blood should be collected in heparinized Vacutainers. Cord blood should be collected in ACD.

Should the anticoagulant be washed off before using RosetteSep™?

No, the antibody cocktail can be added directly to the sample.

更多信息

更多信息
Species Human
Magnet Compatibility  
Sample Source Cord Blood
Selection Method Negative
标记抗体
  1. 抗人CD45抗体，克隆HI30
    抗人CD45抗体，克隆HI30

    小鼠单克隆IgG1抗体，抗人、黑猩猩CD45

  2. 抗人CD34抗体，克隆581
    抗人CD34抗体，克隆581

    小鼠单克隆IgG1抗体，抗人CD34

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