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用于体外诊断(IVD)应用的密度梯度离心管

用于体外诊断(IVD)应用的密度梯度离心管

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产品号 #84850

50mL密度梯度离心管，内含插件

产品优势

  • 无需小心地将血液分层铺在密度梯度离心液（如Lymphoprep™等）上
  • 使用新鲜样本时，总离心时间可缩短至10分钟（打开刹车）
  • 只需简单倾倒上清液即可快速轻松地收集分离的单个核细胞
  • 可与RosetteSep™富集试剂配合使用，在30分钟内分离特定的细胞类型

产品组分包括

  • SepMate™-50 (IVD)，100支装（产品号 #85450)
  • 每盒含4袋，每袋25支管
  • SepMate™-50 (IVD)，500支装（产品号 #85460）
  • 每盒含4袋，每袋25支管（产品号 #85450）× 5盒
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    1. 淋巴制剂™
      淋巴制剂™

      Density gradient medium for the isolation of mononuclear cells

    What Our Scientist Says

    Traditional isolation of PBMCs requires careful layering of blood onto density gradient media prior to centrifugation. We developed SepMate™ to simplify this process, so anyone can isolate PBMCs with a simple pour while maintaining consistency across samples.

    Peter MorinTechnical Scientist
    Peter Morin, Technical Scientist
    概述
    实验数据
    产品说明书及文档
    应用领域
    相关材料与文献

    概述

    将SepMate™整合到密度梯度离心步骤中以简化外周血单个核细胞(PBMC)分离流程。
    SepMate™离心管配有内部插件，可在密度梯度离心液与血样之间形成屏障，既省去精
    细的分层加样操作，又能通过简单倾倒轻松收获单个核细胞。本产品可与RosetteSep™
    联用，分离特定免疫细胞亚群。
    SepMate™按照cGMP标准生产，并在澳大利亚、加拿大、欧洲和美国注册为体外诊断
    (IVD)设备。在中国，该产品被中国食品药品监督管理局（CFDA）认定为非医疗器械，
    因此可作为一般实验室耗材使用。终端用户需自行评估该产品是否适用于其特定用途。
    查阅SepMate™常见问题解答(FAQs)

    CONTAINS
    Polypropylene tube containing an insert
     
    SUBTYPE
    Centrifugation Tubes
     
    CELL TYPE
    B Cells, Dendritic Cells, Monocytes, Mononuclear Cells, NK Cells, T Cells, T Cells, CD4+, T Cells, CD8+, T Cells, Other Subsets, T Cells, Regulatory
     
    SPECIES
    Human
     
    SAMPLE SOURCE
    Bone Marrow, Whole Blood
     
    SELECTION METHOD
    Negative
     
    APPLICATION
    Cell Isolation, In Vitro Diagnostic
     
    BRAND
    SepMate
     
    AREA OF INTEREST
    Chimerism, HLA, Immunology

    实验数据

    PBMC recovery from fresh whole blood using SepMate™-50 versus standard density gradient centrifugation. Graph also shows PBMC recovery from a 48 hour-old sample using SepMate™. n in each group = 7

    Figure 1. Recovery of mononuclear cells (MNCs) from peripheral blood using SepMate™-50 versus standard density gradient centrifiguation. Recovery of MNCs from fresh and 48-hour post blood draw enriched by density gradient centrifugation with SepMate™ (purple) or without (grey). There was no significant difference in the recovery of MNCS with and without SepMate™.

    PBMC recovery from fresh whole blood using SepMate™-50 versus standard density gradient centrifugation. Graph also shows PBMC recovery from a 48 hour-old sample using SepMate™. n in each group = 7

    Figure 2. Human CD4+ T Cell Isolation using SepMate™-50 and RosetteSep™ Human CD4+ T Cell Enrichment Cocktail

    产品说明书及文档

    Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

    Document Type
    Product Name
    Catalog #
    Lot #
    Language
    Document Type
    Product Information Sheet
    Product Name
    SepMate™-50 (IVD)
    Catalog #
    85450, 85460
    Lot #
    All
    Language
    MULTI

    相关材料与文献

    Educational Materials (13)

    SepMate™ Hassle-Free PBMCs in Just 15 Minutes
    Brochure
    SepMate™ Hassle-Free PBMCs in Just 15 Minutes
    Lymphoprep™ Density Gradient Medium for Mononuclear Cell Isolation
    Brochure
    Lymphoprep™ Density Gradient Medium for Mononuclear Cell Isolation
    How to Isolate Mononuclear Cells from Blood in 15 Minutes with SepMate™
    Protocol
    How to Isolate Mononuclear Cells from Blood in 15 Minutes with SepMate™
    How to Speed up Your PBMC Isolations with SepMate™
    18:36
    Video
    How to Speed up Your PBMC Isolations with SepMate™
    Cell Isolation in One Spin Using SepMate™ Tubes and RosetteSep™ Cell Separation Reagents
    1:02
    Video
    Cell Isolation in One Spin Using SepMate™ Tubes and RosetteSep™ Cell Separation Reagents
    How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute Protocol
    1:46
    Video
    How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute Protocol
    How SepMate™ PBMC Isolation Tubes Work: From Whole Blood to PBMCs in 15 Minutes
    2:28
    Video
    How SepMate™ PBMC Isolation Tubes Work: From Whole Blood to PBMCs in 15 Minutes
    Streamlining PBMC Isolation with Automation: A Biobank’s Validation Experience
    51:35
    Webinar
    Streamlining PBMC Isolation with Automation: A Biobank’s Validation Experience
    Fast and Easy Hematopoietic Progenitor Cell Enrichment With SepMate™
    Scientific Poster
    Fast and Easy Hematopoietic Progenitor Cell Enrichment With SepMate™
    A Specialized Tube to Make Rosettesep™ Enrichment of Specific Cell Subsets Faster and Easier
    Scientific Poster
    A Specialized Tube to Make Rosettesep™ Enrichment of Specific Cell Subsets Faster and Easier
    A Specialized Tube to Make Enrichment of Specific Cell Subsets Faster and Easier
    Scientific Poster
    A Specialized Tube to Make Enrichment of Specific Cell Subsets Faster and Easier
    A Specialized Tube for Rapid PBMC Preparation, Pre-Enrichment, or Isolation of Specific Cell Populations
    Scientific Poster
    A Specialized Tube for Rapid PBMC Preparation, Pre-Enrichment, or Isolation of Specific Cell Populat...
    Human Immune Cell Isolation Course
    On-Demand Training
    Human Immune Cell Isolation Course

    Publications (44)

    Profiling HPV-16-specific T cell responses reveals broad antigen reactivities in oropharyngeal cancer patients. K. H. Bhatt et al. The Journal of experimental medicine 2020 oct

    Abstract

    Cellular immunotherapeutics targeting the human papillomavirus (HPV)-16 E6 and E7 proteins have achieved limited success in HPV-positive oropharyngeal cancer (OPC). Here we have conducted proteome-wide profiling of HPV-16-specific T cell responses in a cohort of 66 patients with HPV-associated OPC and 22 healthy individuals. Unexpectedly, HPV-specific T cell responses from OPC patients were not constrained to the E6 and E7 antigens; they also recognized E1, E2, E4, E5, and L1 proteins as dominant targets for virus-specific CD8+ and CD4+ T cells. Multivariate analysis incorporating tumor staging, treatment status, and smoking history revealed that treatment status had the most significant impact on HPV-specific CD8+ and CD4+ T cell immunity. Specifically, the breadth and overall strength of HPV-specific T cell responses were significantly higher before the commencement of curative therapy than after therapy. These data provide the first glimpse of the overall human T cell response to HPV in a clinical setting and offer groundbreaking insight into future development of cellular immunotherapies for HPV-associated OPC patients.
    View publication
    Vaccination of koalas during antibiotic treatment for Chlamydia-induced cystitis induces an improved antibody response to Chlamydia pecorum. S. Phillips et al. Scientific reports 2020 jun

    Abstract

    Chlamydia infection and disease are endemic in free-ranging koalas. Antibiotics remain the front line treatment for Chlamydia in koalas, despite their rates of treatment failure and adverse gut dysbiosis outcomes. A Chlamydia vaccine for koalas has shown promise for replacing antibiotic treatment in mild ocular Chlamydia disease. In more severe disease presentations that require antibiotic intervention, the effect of vaccinating during antibiotic use is not currently known. This study investigated whether a productive immune response could be induced by vaccinating koalas during antibiotic treatment for Chlamydia-induced cystitis. Plasma IgG antibody levels against the C. pecorum major outer membrane protein (MOMP) dropped during antibiotic treatment in both vaccinated and unvaccinated koalas. Post-treatment, IgG levels recovered. The IgG antibodies from naturally-infected, vaccinated koalas recognised a greater proportion of the MOMP protein compared to their naturally-infected, unvaccinated counterparts. Furthermore, peripheral blood mononuclear cell gene expression revealed an up-regulation in genes related to neutrophil degranulation in vaccinated koalas during the first month post-vaccination. These findings show that vaccination of koalas while they are being treated with antibiotics for cystitis can result in the generation of a productive immune response, in the form of increased and expanded IgG production and host response through neutrophil degranulation.
    View publication
    The Effect of Bovine Viral Diarrhea Virus (BVDV) Strains and the Corresponding Infected-Macrophages' Supernatant on Macrophage Inflammatory Function and Lymphocyte Apoptosis. K. Abdelsalam et al. Viruses 2020 jun

    Abstract

    Bovine viral diarrhea virus (BVDV) is an important viral disease of cattle that causes immune dysfunction. Macrophages are the key cells for the initiation of the innate immunity and play an important role in viral pathogenesis. In this in vitro study, we studied the effect of the supernatant of BVDV-infected macrophage on immune dysfunction. We infected bovine monocyte-derived macrophages (MDM) with high or low virulence strains of BVDV. The supernatant recovered from BVDV-infected MDM was used to examine the functional activity and surface marker expression of normal macrophages as well as lymphocyte apoptosis. Supernatants from the highly virulent 1373-infected MDM reduced phagocytosis, bactericidal activity and downregulated MHC II and CD14 expression of macrophages. Supernatants from 1373-infected MDM induced apoptosis in MDBK cells, lymphocytes or BL-3 cells. By protein electrophoresis, several protein bands were unique for high-virulence, 1373-infected MDM supernatant. There was no significant difference in the apoptosis-related cytokine mRNA (IL-1beta, IL-6 and TNF-a) of infected MDM. These data suggest that BVDV has an indirect negative effect on macrophage functions that is strain-specific. Further studies are required to determine the identity and mechanism of action of these virulence factors present in the supernatant of the infected macrophages.
    View publication
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    Legal Statement: SepMate™ (IVD) is only available in regions where it is registered as an In Vitro Diagnostic (IVD) device for the isolation of MNCs from whole blood or bone marrow by density gradient centrifugation. SepMate™ is manufactured under a cGMP quality managment system compliant to 21 CFR 820. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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