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RosetteSep™人脐带血减积抗体混合物

免疫密度负选试剂混合物
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产品号 #15126_C

免疫密度负选试剂混合物

产品优势

  • 快捷、操作简单
  • 不需要特殊设备或额外培训
  • 获得的活细胞无标记
  • 可与SepMate™联合使用，实现一致的高通量样本处理

产品组分包括

  • RosetteSep™人脐带血减积抗体混合物（产品号#15126）
    • RosetteSep™人类去杂质混合液，2mL
  • RosetteSep™人脐带血减积抗体混合物（产品号#15166）
    • RosetteSep™人类去杂质混合液，5 x 2mL
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Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. Lymphoprep™
    Lymphoprep™

    Density gradient medium for the isolation of mononuclear cells

  2. Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum
    Dulbecco's Phosphate Buffered Saline with 2% ...

    Cell culture buffer

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

概述

RosetteSep™人脐带血减积抗体混合物用于从新鲜脐带血去除谱系阳性细胞。四聚体抗体复合物可识别 CD2、CD14、 CD19、CD66b以及红细胞（RBC）上的糖蛋白A，从而靶向去除非目的细胞。使用密度梯度离心液如Lymphoprep™（产品号 #07801）离心后，非目的细胞会与红细胞一起沉淀。造血干细胞和祖细胞为血浆和密度梯度离心液的交界界面中高度富集的细胞。

Subtype
Cell Isolation Kits
 
Cell Type
Hematopoietic Stem and Progenitor Cells
 
Species
Human
 
Sample Source
Cord Blood
 
Selection Method
Depletion
 
Application
Cell Isolation
 
Brand
RosetteSep
 
Area of Interest
Immunology, Stem Cell Biology
 

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Data Figures

FACS Profile Results with RosetteSep™ De-Bulking Cocktail

Figure 1. FACS Profile Results with RosetteSep™ De-Bulking Cocktail

Starting with fresh cord blood, the enrichment of CD34+ cells is typically 5 ± 1%.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RosetteSep™ Human Cord Blood Debulking Cocktail
Catalog #
15166, 15126
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
RosetteSep™ Human Cord Blood Debulking Cocktail
Catalog #
15166, 15126
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (3)

Human Immune Cytokines
Wallchart
Human Immune Cytokines
Isolate Highly Purified Cells for HLA Analysis with RosetteSep™ Immunodensity Cell Isolation
1:21
Video
Isolate Highly Purified Cells for HLA Analysis with RosetteSep™ Immunodensity Cell Isolation
Isolate Cells from Whole Blood without Columns or Magnets: RosetteSep™ Immunodensity Cell Separation
2:19
Video
Isolate Cells from Whole Blood without Columns or Magnets: RosetteSep™ Immunodensity Cell Separati...

Frequently Asked Questions

What is RosetteSep™?

RosetteSep™ is a rapid cell separation procedure for the isolation of purified cells directly from whole blood, without columns or magnets.

How does RosetteSep™ work?

The antibody cocktail crosslinks unwanted cells to red blood cells (RBCs), forming rosettes. The unwanted cells then pellet with the free RBCs when centrifuged over a density centrifugation medium (e.g. Ficoll-Paque™ PLUS, Lymphoprep™).

What factors affect cell recovery?

The temperature of the reagents can affect cell recovery. All reagents should be at room temperature (sample, density centrifugation medium, PBS, centrifuge) before performing the isolations. Layering can also affect recovery so be sure to carefully layer the sample to avoid mixing with the density centrifugation medium as much as possible. Be sure to collect the entire enriched culture without disturbing the RBC pellet. A small amount of density centrifugation medium can be collected without worry.

Which cell samples can RosetteSep™ be used with?

RosetteSep™ can be used with leukapheresis samples, bone marrow or buffy coat, as long as: the concentration of cells does not exceed 5 x 107 per mL (can dilute if necessary); and there are at least 100 RBCs for every nucleated cell (RBCs can be added if necessary).

Can RosetteSep™ be used with previously frozen or cultured cells?

Yes. Cells should be re-suspended at 2 - 5 x 107 cells / mL in PBS + 2% FBS. Fresh whole blood should be added at 250 µL per mL of sample, as a source of red cells.

Can RosetteSep™ be used to enrich progenitors from cord blood?

Yes. Sometimes cord blood contains immature nucleated red cells that have a lower density than mature RBCs. These immature red cells do not pellet over Ficoll™, which can lead to a higher RBC contamination than peripheral blood separations.

Does RosetteSep™ work with mouse cells?

No, but we have developed EasySep™, a magnetic-based cell isolation system which works with mouse and other non-human species.

Which anticoagulant should be used with RosetteSep™?

Peripheral blood should be collected in heparinized Vacutainers. Cord blood should be collected in ACD.

Should the anticoagulant be washed off before using RosetteSep™?

No, the antibody cocktail can be added directly to the sample.

更多信息

更多信息
Species Human
Sample Source Cord Blood
Selection Method Depletion
标记抗体
  1. 抗人CD34抗体，克隆581
    抗人CD34抗体，克隆581

    小鼠抗人CD34单克隆IgG1抗体

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