The RosetteSep™ Human Cord Blood Progenitor Cell Enrichment Cocktail with HetaSep™ is designed to isolate progenitor cells from cord blood by negative selection. The HetaSep™ minimizes the required volume of the RosetteSep™ cocktail reagent by first concentrating the sample by sedimentation with HetaSep™ (Catalog #07906), a solution of hetastarch. The hematopoietic progenitors in the resultant buffy coat are then enriched using a modified RosetteSep™ protocol that uses less RosetteSep™ cocktail than is required for an unconcentrated sample. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD3, CD14, CD16, CD19, CD24, CD56, CD61, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a density gradient medium the unwanted cells pellet along with the RBCs. The purified progenitor cells are present as a highly enriched population at the interface between the plasma and the density gradient medium.
We regret to inform our customers that we can no longer include Ficoll-Paque™ Plus with this product. For performing cell isolation by density gradient centrifugation, we recommend purchasing Lymphoprep™ (a href="https://www.stemcell.com/products/lymphoprep.html">Catalog #18060), which is available in a variety of sizes and formats. As with Ficoll-Paque™, Lymphoprep™ has a density of 1.077 g/mL, and after centrifugation, mononuclear cells remain at the plasma-Lymphoprep™ interface while granulocytes and erythrocytes sediment below.
We regret to inform our customers that we can no longer include Ficoll-Paque™ Plus with this product. For performing cell isolation by density gradient centrifugation, we recommend purchasing Lymphoprep™ (a href="https://www.stemcell.com/products/lymphoprep.html">Catalog #18060), which is available in a variety of sizes and formats. As with Ficoll-Paque™, Lymphoprep™ has a density of 1.077 g/mL, and after centrifugation, mononuclear cells remain at the plasma-Lymphoprep™ interface while granulocytes and erythrocytes sediment below.
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
