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RosetteSep™人脐带血祖细胞完全富集试剂盒

免疫密度负选试剂
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产品号 #（选择产品）

产品号 #15276_C

包含RosetteSep™人脐带血祖细胞试剂盒和HetaSep™

产品优势

  •  易于操作、快速
  • 无需特殊设备或培训
  • 获得的活细胞无标记

产品组分包括

  • RosetteSep™人脐带血祖细胞富集试剂盒（产品号#15276）
  • RosetteSep™人造血祖细胞富集抗体混合物，2 mL (产品号#15026) x 2
  • HetaSep™，100 mL（产品号#07906）
main product photo
专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. Lymphoprep™
    Lymphoprep™
  2. DPBS（含 2% 胎牛血清）
    DPBS（含 2% 胎牛血清）
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

含HetaSep™的RosetteSep™人造血祖细胞富集抗体混合物，设计用来从脐带血中负选祖细胞。HetaSep™通过首先使用羟乙基淀粉溶液HetaSep™（产品号#07906）进行沉降浓缩样本，从而减少所需的RosetteSep™抗体实际的用量。随后采用改良的RosetteSep™方案富集所得白膜层中的造血祖细胞，该方案比未浓缩样本所需的RosetteSep™混合试剂量更少。四聚体抗体复合物可识别CD2, CD3, CD14, CD16, CD19, CD24, CD56, CD61, CD66b和红细胞（RBCs）上的糖蛋白A，从而靶向去除非目的细胞。当在密度梯度介质上进行离心时，不需要的细胞会与红细胞一起沉降到底部。纯化后的祖细胞则作为高度富集的细胞群，出现在血浆和密度梯度离心液的界面上。

我们遗憾地通知客户，本产品将不再附带 Ficoll-Paque™ Plus。若需通过密度梯度离心进行细胞分离，我们建议您另行购买 Lymphoprep™（产品号：#18060），该产品提供多种规格和包装选择。与 Ficoll-Paque™ 相同，Lymphoprep™ 的密度为 1.077 g/mL，离心后，单个核细胞保留在血浆与 Lymphoprep™ 的界面处，而粒细胞和红细胞则沉降到下方。

亚型
细胞分选试剂盒
 
细胞类型
造血干/祖细胞
 
种属
人
 
样本来源
Cord Blood
 
筛选方法
Negative
 
应用
细胞分选
 
品牌
RosetteSep
 
研究领域
免疫，干细胞生物学
 

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Data Figures

FACS Profile Results With RosetteSep™ Human Cord Blood Progenitor CellEnrichment Kit

Figure 1. FACS Profile Results With RosetteSep™ Human Cord Blood Progenitor Cell Enrichment Kit

Starting with fresh cord blood, the enrichment of CD34+ cells is typically 29 ± 9%.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Complete RosetteSep™ Human Cord Blood Progenitor Enrichment Kit
Catalog #
15276
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
Complete RosetteSep™ Human Cord Blood Progenitor Enrichment Kit
Catalog #
15276
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
Complete RosetteSep™ Human Cord Blood Progenitor Enrichment Kit
Catalog #
15276
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (6)

Cell Separation Products for HLA Analysis
Brochure
Cell Separation Products for HLA Analysis
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Isolate Cells from Whole Blood without Columns or Magnets: RosetteSep™ Immunodensity Cell Separation
2:19
Video
Isolate Cells from Whole Blood without Columns or Magnets: RosetteSep™ Immunodensity Cell Separati...
How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute Protocol
1:46
Video
How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute Protocol
Fast and Easy Hematopoietic Progenitor Cell Enrichment With SepMate™
Scientific Poster
Fast and Easy Hematopoietic Progenitor Cell Enrichment With SepMate™

Frequently Asked Questions

What is RosetteSep™?

RosetteSep™ is a rapid cell separation procedure for the isolation of purified cells directly from whole blood, without columns or magnets.

How does RosetteSep™ work?

The antibody cocktail crosslinks unwanted cells to red blood cells (RBCs), forming rosettes. The unwanted cells then pellet with the free RBCs when centrifuged over a density centrifugation medium (e.g. Ficoll-Paque™ PLUS, Lymphoprep™).

What factors affect cell recovery?

The temperature of the reagents can affect cell recovery. All reagents should be at room temperature (sample, density centrifugation medium, PBS, centrifuge) before performing the isolations. Layering can also affect recovery so be sure to carefully layer the sample to avoid mixing with the density centrifugation medium as much as possible. Be sure to collect the entire enriched culture without disturbing the RBC pellet. A small amount of density centrifugation medium can be collected without worry.

Which cell samples can RosetteSep™ be used with?

RosetteSep™ can be used with leukapheresis samples, bone marrow or buffy coat, as long as: the concentration of cells does not exceed 5 x 107 per mL (can dilute if necessary); and there are at least 100 RBCs for every nucleated cell (RBCs can be added if necessary).

Can RosetteSep™ be used with previously frozen or cultured cells?

Yes. Cells should be re-suspended at 2 - 5 x 107 cells / mL in PBS + 2% FBS. Fresh whole blood should be added at 250 µL per mL of sample, as a source of red cells.

Can RosetteSep™ be used to enrich progenitors from cord blood?

Yes. Sometimes cord blood contains immature nucleated red cells that have a lower density than mature RBCs. These immature red cells do not pellet over Ficoll™, which can lead to a higher RBC contamination than peripheral blood separations.

Does RosetteSep™ work with mouse cells?

No, but we have developed EasySep™, a magnetic-based cell isolation system which works with mouse and other non-human species.

Which anticoagulant should be used with RosetteSep™?

Peripheral blood should be collected in heparinized Vacutainers. Cord blood should be collected in ACD.

Should the anticoagulant be washed off before using RosetteSep™?

No, the antibody cocktail can be added directly to the sample.

更多信息

更多信息
Species Human
Sample Source Cord Blood
Selection Method Negative
标记抗体
  1. 抗人CD34抗体，克隆8G12
    抗人CD34抗体，克隆8G12

    小鼠单克隆IgG1抗体，抗人CD34

  2. 抗人CD34抗体，克隆581
    抗人CD34抗体，克隆581

    小鼠单克隆IgG1抗体，抗人CD34

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