产品号 #76013_C
CRISPR-Cas9基因组编辑阳性对照
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
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ArciTect™ Cas9 NucleaseCas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing
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ArciTect™ T7 Endonuclease I KitFor estimation of CRISPR-Cas9 genome editing efficiency
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ArciTect™ High-Fidelity DNA Polymerase KitPolymerase, buffers, and dNTPs for high-fidelity PCR amplification
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Nuclease-Free WaterDNase- and RNase-free water for molecular biology applications
概述
ArciTect™人类HPRT阳性对照试剂盒设计为使用ArciTect™CRISPR-Cas9基因组编辑系统进行实验的阳性对照。该试剂盒包括architect™Human HPRT crRNA （2 nmol）、architect™Human HPRT Primer Mix （2 nmol）、architect™tracrRNA （5 nmol）和architect™退火缓冲液（5X）。architect™Human HPRT crRNA首先需要退火到architect™tracrRNA（包含在试剂盒中）。然后必须将其与architect™Cas9核酸酶（例如目录#76002）结合，形成核糖核蛋白复合物。architect™Human HPRT引物Mix可用于扩增从转染细胞群体中分离的基因组DNA，随后可用于T7核酸内切酶I测定以确定切割效率。
该试剂盒已经过测试和验证，可与architect™系列基因组编辑产品一起使用。HPRT（次黄嘌呤磷酸核糖基转移酶）是一种管家基因，也是一种常用的对照基因。该试剂盒可用于优化转染方案，并作为阳性对照，可与定制的architect™crrna（例如目录#76010）一起使用。
Cell Type
Pluripotent Stem Cells
Species
Human
Application
Genome Editing
Area of Interest
Stem Cell Biology
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (6)
更多信息
| Species | Human |
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健康对照人 iPSC 系，女性，SCTi003-A人多能干细胞系，冷冻
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architect™T7核酸内切酶I检测试剂盒用于估计CRISPR-Cas9基因组编辑效率
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基因组DNA纯化试剂盒用于从细胞或组织中纯化基因组DNA
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凝胶和PCR清理试剂盒用于琼脂糖凝胶中DNA的提取，或PCR产物和限制性内切酶消化的清理
Legal Statement: The purchase of the ArciTect™ products conveys to the purchaser the limited, non-transferable right to use, in accordance with all applicable laws and regulations, the ArciTect™ products and any related material solely for research purposes only, not for any clinical, human, agricultural, veterinary, livestock, or commercial purpose, with no warranty (express or implied) from the owners or assignees of the Patents or STEMCELL Technologies each of whom expressly disclaim any warranty regarding results obtained through the use of the ArciTect™ products, and without any exception the owners or assignees of the Patents or STEMCELL Technologies, or as applicable a director, trustee, officer, employee, agent, faculty, official investigator or student thereof, will not suffer or be exposed to any liability, damages, loss, or expense of any kind whatsoever arising from or related to the use of the ArciTect™ products by a purchaser of same. Distribution of ArciTect™ products by STEMCELL Technologies is covered under at least US 8,697,359, US 8,771,945, US 8,795,965, US 8,865,406, US 8,871,445, US 8,889,356, US 8,889,418, US 8,895,308, US 8,906,616 and foreign equivalents (“Patents”). PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
