Positive control for CRISPR-Cas9 genome editing
Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing
For estimation of CRISPR-Cas9 genome editing efficiency
Polymerase, buffers, and dNTPs for high-fidelity PCR amplification
DNase- and RNase-free water for molecular biology applications
ArciTect™ Human HPRT Positive Control Kit is designed as a positive control for experiments using the ArciTect™ CRISPR-Cas9 genome editing system. The kit comprises ArciTect™ Human HPRT crRNA (2 nmol), ArciTect™ Human HPRT Primer Mix (2 nmol), ArciTect™ tracrRNA (5 nmol), and ArciTect™ Annealing Buffer (5X). ArciTect™ Human HPRT crRNA first requires annealing to ArciTect™ tracrRNA (included in the kit). This must then be combined with an ArciTect™ Cas9 Nuclease (e.g. Catalog #76002) to form a ribonucleoprotein complex. ArciTect™ Human HPRT Primer Mix can be used to amplify genomic DNA isolated from a population of transfected cells, which can subsequently be used in a T7 endonuclease I assay to determine cleavage efficiency.
This kit has been tested and validated for use with the ArciTect™ line of genome editing products. HPRT, or hypoxanthine phosphoribosyltransferase, is a housekeeping gene and a commonly used control. The kit can be used to optimize transfection protocols and act as a positive control that can be used alongside custom ArciTect™ crRNAs (e.g. Catalog #76010)
Cell Type
Pluripotent Stem Cells
Species
Human
Application
Genome Editing
Area of Interest
Stem Cell Biology
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
