ArciTect™ 高保真 DNA 聚合酶试剂盒

用于高保真 PCR 扩增的聚合酶、缓冲液和 dNTP

产品号 #(选择产品)

产品号 #76026_C

用于高保真 PCR 扩增的聚合酶、缓冲液和 dNTP

产品优势

  • ArciTect™ 高保真 DNA 聚合酶,250 µL
  • ArciTect™ 高保真缓冲液,6 x 1.5 mL
  • ArciTect™ 高 GC 含量缓冲液,3 x 1.5 mL
  • dNTP 混合物,1 mL

产品组分包括

  • ArciTect™ High-Fidelity DNA Polymerase, 250 µL
  • ArciTect™ High-Fidelity Buffer, 6 x 1.5 mL
  • ArciTect™ High GC Content Buffer, 3 x 1.5 mL
  • dNTP Mix, 1 mL
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总览

ArciTect™ 高保真 DNA 聚合酶试剂盒经过优化,可实现稳定、高保真 DNA 扩增(保真度比 Taq 聚合酶高出 50 倍以上)。该试剂盒适用于对超低错误率有要求的应用,如结合 ArciTect™ T7 核酸内切酶 I 进行基因组编辑检测(请参阅技术公告:基因组编辑评估),以及测序、克隆/亚克隆、合成生物学和 SNP 分析。ArciTect™ 高保真 DNA 聚合酶是一种融合蛋白,包含双链 DNA 结合结构域和类焦球菌校对聚合酶结构域。
ArciTect™ 高保真 DNA 聚合酶试剂盒包含 ArciTect™ 高保真 DNA 聚合酶、dNTP 混合物(10 mM;含 dATP、dCTP、dGTP 和 dTTP 钠盐)以及两种缓冲液:ArciTect™ 高保真缓冲液(用于标准高保真反应)和 ArciTect™ 高 GC 含量缓冲液(用于难以扩增且富含 G 和 C 碱基的模板)。使用 ArciTect™ 高 GC 含量缓冲液的反应灵敏度更高,但错误率略高。所有成分均不含动物成分。
在乳糖苷酶 (lacl)诱变实验中,ArciTect™ 高保真 DNA 聚合酶的错误率比标准Taq聚合酶低 50 到 60 倍。其延伸速率为每秒 67 个核苷酸,并可成功扩增较长的目标片段(可扩增最长达 5 kb 的人类基因组 DNA 或 8 kb 的 λ 噬菌体 DNA)。该酶产生平末端(blunt-end)产物。

细胞类型
多能干细胞
 
种属
人,小鼠,大鼠
 
应用
基因组编辑
 
研究领域
疾病建模,干细胞生物学
 

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
76026
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
76026
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
76026
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
76026
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

更多信息

更多信息
Species Human, Mouse, Rat
Legal Statement: Sale of this product is covered under at least US Patent Nos. 6,627,424, 7,541,170, 7,560,260, and corresponding patents in other countries or other patents pending and all applicable STEMCELL Terms and Conditions of Sale. This product can be used solely for DNA sequencing, DNA micro-array and conventional PCR applications in the life science research field, but not real-time PCR or digital PCR. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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