产品号 #76026_C
用于高保真PCR扩增的聚合酶、缓冲液和dNTPs
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Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
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Nuclease-Free WaterDNase- and RNase-free water for molecular biology applications
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Genomic DNA Purification KitFor purification of genomic DNA from cells or tissue
概述
architect™高保真DNA聚合酶试剂盒是优化的稳健，高保真DNA扩增(bbb50倍比Taq）. 它可用于需要超低错误率的应用，例如使用architect™T7核酸内切酶I检测基因组编辑(参见技术公报：基因组编辑的评价)，以及测序、克隆/亚克隆、合成生物学和SNP分析。architect™高保真DNA聚合酶是一种融合蛋白，具有双链DNA结合域和a海床比如校对聚合酶结构域。
architect™高保真DNA聚合酶试剂盒包括architect™高保真DNA聚合酶，dNTP混合物(10 mM；含有dATP， dCTP， dGTP和dTTP钠盐)，以及两种缓冲液：architect™高保真缓冲液（用于标准高保真反应）和architect™高GC含量缓冲液（用于富含G和C碱基的难以扩增的模板）。使用architect™高GC含量缓冲液的反应增加了灵敏度，但错误率略高。所有组件均不含动物成分。
architect™高保真DNA聚合酶的错误率比标准产品低50- 60倍Taq聚合酶使用局部诱变试验。它具有每秒67个核苷酸的扩展速率，可以成功地扩增长目标（多达5kb的人类基因组DNA和8kb的lambda DNA）。这种酶产生钝端产物。
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Cell Type
Pluripotent Stem Cells
Species
Human, Mouse, Rat
Application
Genome Editing
Area of Interest
Disease Modeling, Stem Cell Biology
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (8)
更多信息
| Species | Human, Mouse, Rat |
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Legal Statement: Sale of this product is covered under at least US Patent Nos. 6,627,424, 7,541,170, 7,560,260, and corresponding patents in other countries or other patents pending and all applicable STEMCELL Terms and Conditions of Sale. This product can be used solely for DNA sequencing, DNA micro-array and conventional PCR applications in the life science research field, but not real-time PCR or digital PCR. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
