心肌细胞分化试剂盒(人)

人骨骼肌祖细胞(成肌细胞)分化为肌管的培养基

产品号 #(选择产品)

产品号 #05965_C

人骨骼肌祖细胞(成肌细胞)分化为肌管的培养基

产品优势

  • 支持肌原性祖细胞向多核肌管的分化,强烈表达肌球蛋白重链和肌原素标记物
  • 严格的原材料筛选和质量控制,最大限度地减少了批次之间的差异
  • 附着底物包括支持最佳粘附和增强肌管形态

产品组分包括

  • 心肌™分化基础培养基(人),190 mL
  • 心肌™分化20X补充物(人),10毫升
  • ACF细胞附着底物,1ml

概述

心肌™分化试剂盒(人)包括心肌™分化培养基和动物无组分(ACF)细胞附着底物。心肌分化培养基是一种基于血清的培养基,专门用于人类骨骼肌祖细胞(成肌细胞)在体外分化为肌管。心肌分化培养基设计用于ACF细胞附着底物,该底物已经过专门测试,其支持最佳粘附和肌管形态的能力。该培养基适用于先前在含血清或无血清培养基中扩增的肌源性祖细胞的分化(例如使用myult™-SF扩增试剂盒#05980)。

CollPlant是细胞附着底物的rhCollagen成分的制造商。

本产品仅可用于研究目的。有关本产品的任何临床或商业应用,请联系STEMCELL。

Subtype
Specialized Media
 
Cell Type
Myogenic Stem and Progenitor Cells
 
Species
Human
 
Application
Cell Culture, Differentiation
 
Brand
MyoCult
 
Area of Interest
Disease Modeling
 

Data Figures

Figure 1. MyoCult™ Differentiation Medium Induces Robust Differentiation of Myotubes from Human Myoblasts

(A) Myogenic progenitor cells expanded in MyoCult expansion medium expressed MyoD (green) and desmin (red). (B) Robust differentiation of myoblasts (shown in (A)) to multinucleated myotubes expressing myosin heavy chain (MyHC+) was achieved following culture in MyoCult™ Differentiation Medium for as little as 5 days. (C) A fusion index showing approximately 75% of total nuclei were found within myotubes expressing MyHC.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
05965
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
05965
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
05965
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
05965
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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Species Human
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