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EasySep™人CD56正选试剂盒 II

人CD56+细胞的免疫磁珠正选
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产品号 #（选择产品）

产品号 #17855_C

人CD56+细胞的免疫磁珠正选

产品优势

  • 快捷、操作简单    
  • 纯度高达98%
  • 无需分离柱     

产品组分包括

  • EasySep™人CD56正选试剂盒 II（产品号 #17855）
    • EasySep™人CD56正选抗体混合物 II，1 mL
    • EasySep™ Dextran RapidSpheres™ 50100 磁珠，1 mL
  • RoboSep™人CD56正选试剂盒 II（产品号 #17855RF）
    • EasySep™人CD56正选抗体混合物 II，1 mL
    • EasySep™ Dextran RapidSpheres™ 50100 磁珠，1 mL
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™过滤吸头（产品号 #20125）
main product photo
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

使用EasySep™人CD56正选试剂盒 II，通过免疫磁珠正选 ，从新鲜或冻存的外周血单个核细胞或人骨骼肌（成肌细胞和成纤维细胞）培养样本中分离高纯度的人CD56+细胞。EasySep™结合了单克隆抗体的特异性和无柱磁性系统的简便性，迄今已广泛应用于发表的研究中超过20年。    

在此EasySep™正选过程中，目的细胞被识别CD56的抗体复合物及磁珠结合标记。该试剂盒中还含有人Fc受体阻断剂 ，可最大程度减少非特异性结合。使用EasySep™磁极分离标记的目的细胞，只需倾倒或吸弃非目的细胞，目标细胞则保留在管中。经磁珠分选后的目的CD56+细胞可直接用于下游应用，如流式细胞术、细胞培养或DNA/RNA提取。CD56抗原表达于NK细胞、NKT细胞和人成肌细胞表面 。

该产品可替代EasySep™人CD56细胞正选试剂盒 (产品号 #18055 ) 以进行更快的细胞分选。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现全自动化免疫磁珠细胞分选。或选择即用型、符合伦理规范的冻存人外周血CD56+原代细胞，该细胞通过EasySep™人CD56正选试剂盒 II分离获得。探索更多优化您实验流程的产品     ，包括培养基、添加剂、抗体等。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyPlate™ EasySep™ Magnet (Catalog #18102) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
NK 细胞
 
种属
人
 
样本来源
其它细胞系，PBMC
 
筛选方法
Positive
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
嵌合体，免疫
 

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Data Figures

Typical EasySep™ Human CD56 Positive Selection Profile

Figure 1. Typical EasySep™ Human CD56 Positive Selection Kit II (Catalog #17855)

Starting with human PBMCs, the CD56+ cell content of the isolated fraction is typically 96.3 ± 2.4% (mean ± SD), using the purple EasySep™ Magnet. In the above example, the purities of the start and final isolated fractions are 8.0% and 98.5%, respectively.

FACS Data for Anti-Human CD56 Antibody, Clone HCD56, Alexa Fluor® 488-Conjugated

Figure 2. FACS Data for Anti-Human CD56 Antibody, Clone HCD56, Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD56 Antibody, Clone HCD56, Alexa Fluor® (Catalog #60021AD) and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ).

(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD56 Positive Selection Kit (Catalog #17885) and labeled with Anti-Human CD56 Clone HCD56, Alexa Fluor® 488. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody is shown (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet - Muscle Culture
Product Name
EasySep™ Human CD56 Positive Selection Kit II
Catalog #
17855
Lot #
1000087747 or higher
Language
English
Document Type
Product Information Sheet - PBMCs
Product Name
EasySep™ Human CD56 Positive Selection Kit II
Catalog #
17855
Lot #
All
Language
English
Document Type
Product Information Sheet - PBMCs
Product Name
RoboSep™ Human CD56 Positive Selection Kit II
Catalog #
17855RF
Lot #
All
Language
English
Document Type
Product Information Sheet - Muscle Culture
Product Name
RoboSep™ Human CD56 Positive Selection Kit II
Catalog #
17855RF
Lot #
1000087747 or higher
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human CD56 Positive Selection Kit II
Catalog #
17855
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human CD56 Positive Selection Kit II
Catalog #
17855
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human CD56 Positive Selection Kit II
Catalog #
17855RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human CD56 Positive Selection Kit II
Catalog #
17855RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human CD56 Positive Selection Kit II
Catalog #
17855RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (11)

Isolate Human Immune Cells
Brochure
Isolate Human Immune Cells
Human NK Cell Product Workflow
Brochure
Human NK Cell Product Workflow
EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Cell Separation Products for HLA Analysis
Brochure
Cell Separation Products for HLA Analysis
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
0:57
Video
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep...
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Cell Separation Solutions for HLA and Chimerism Analysis
26:43
Webinar
Cell Separation Solutions for HLA and Chimerism Analysis

Frequently Asked Questions

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

Publications (2)

PD-1 blockade potentiates HIV latency reversal ex vivo in CD4+ T cells from ART-suppressed individuals. R. Fromentin et al. Nature communications 2019 feb

Abstract

HIV persists in latently infected CD4+ T cells during antiretroviral therapy (ART). Immune checkpoint molecules, including PD-1, are preferentially expressed at the surface of persistently infected cells. However, whether PD-1 plays a functional role in HIV latency and reservoir persistence remains unknown. Using CD4+ T cells from HIV-infected individuals, we show that the engagement of PD-1 inhibits viral production at the transcriptional level and abrogates T-cell receptor (TCR)-induced HIV reactivation in latently infected cells. Conversely, PD-1 blockade with the monoclonal antibody pembrolizumab enhances HIV production in combination with the latency reversing agent bryostatin without increasing T cell activation. Our results suggest that the administration of immune checkpoint blockers to HIV-infected individuals on ART may facilitate latency disruption.
View publication
Myalgic encephalomyelitis/chronic fatigue syndrome patients exhibit altered T cell metabolism and cytokine associations. A. H. Mandarano et al. The Journal of clinical investigation 2019 dec

Abstract

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a complex disease with no known cause or mechanism. There is an increasing appreciation for the role of immune and metabolic dysfunction in the disease. ME/CFS has historically presented in outbreaks, often has a flu-like onset, and results in inflammatory symptoms. Patients suffer from severe fatigue and post-exertional malaise. There is little known about the metabolism of specific immune cells in ME/CFS patients. To investigate immune metabolism in ME/CFS, we isolated CD4+ and CD8+ T cells from 53 ME/CFS patients and 45 healthy controls. We analyzed glycolysis and mitochondrial respiration in resting and activated T cells, along with markers related to cellular metabolism, and plasma cytokines. We found that ME/CFS CD8+ T cells have reduced mitochondrial membrane potential compared to healthy controls. Both CD4+ and CD8+ T cells from ME/CFS patients had reduced glycolysis at rest, while CD8+ T cells also had reduced glycolysis following activation. ME/CFS patients had significant correlations between measures of T cell metabolism and plasma cytokine abundance that differed from healthy control subjects. Our data indicate that patients have impaired T cell metabolism consistent with ongoing immune alterations in ME/CFS that may illuminate the mechanism behind this disease.
View publication
View All Publications

更多信息

更多信息
Species Human
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyPlate™ EasySep™ Magnet (Catalog #18102) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
Sample Source Other, PBMC
Selection Method Positive
标记抗体
  1. 抗人CD56抗体，克隆HCD56
    抗人CD56抗体，克隆HCD56

    小鼠单克隆IgG1抗体，抗人CD56 （NCAM）

  2. 山羊抗小鼠 IgG (H+L) 抗体，多克隆
    山羊抗小鼠 IgG (H+L) 抗体，多克隆

    抗小鼠IgG（H+L）山羊多克隆抗体

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  • ISO 13485 和 ISO 9001 质量管理体系认证
    ISO 14001 环境管理体系认证

Scientists Helping Scientists™

ISO 13485 和 ISO 9001 质量管理体系认证

ISO 14001 环境管理体系认证

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