切换导航
  • 比较产品
我的购物车

小鼠tnf - α酶联免疫吸附测定试剂盒

用于小鼠肿瘤坏死因子- α的检测和测定
率先评论此产品
添加并比较

产品号 #（选择产品）

产品号 #02030_C

用于小鼠肿瘤坏死因子- α的检测和测定

产品组分包括

  • 小鼠tnf - α酶联免疫吸附测定试剂盒，2板（目录#02030）
    • 小鼠TNF-α酶联免疫吸附试验板，2x
    • 小鼠TNF-α标准品，1瓶
    • 小鼠TNF-α生物素化检测抗体，30µL
    • Sa-hrp, 30µl
    • 标准重组缓冲液B， 1ml
    • 洗涤缓冲液（20X）， 120 mL
    • ELISA稀释液，120 mL
    • SA-HRP稀释液，25ml
    • TMB底物，25ml
    • 停止液，25ml
    • 粘接板盖，6个
  • 小鼠tnf - α酶联免疫吸附测定试剂盒，10板（目录#02031）
    • 小鼠TNF-α酶联免疫吸附板，10x
    • 小鼠TNF-α标准品，1瓶
    • 小鼠TNF-α生物素化检测抗体，130µL
    • Sa-hrp, 130µl
    • 标准重组缓冲液B， 1ml
    • 洗涤缓冲液（20倍），5 × 120毫升
    • ELISA稀释剂，3 × 120ml
    • SA-HRP稀释剂，120 mL
    • TMB底物，120 mL
    • 停止液，120ml
    • 胶粘剂板盖，30x
main product photo
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

概述

小鼠肿瘤坏死因子-α (TNF-α)酶联免疫吸附测定试剂盒用于定量检测和测量小鼠血清、血浆和细胞培养上清等生物液体中的TNF-α。TNF-α主要由活化的T细胞和巨噬细胞在炎症或感染反应中产生，但也由B细胞、NK细胞、肥大细胞、中性粒细胞、树突状细胞、小胶质细胞、内皮细胞、平滑肌细胞、心肌细胞和成纤维细胞产生。TNF-α是一种促炎细胞因子，在体外刺激抗肿瘤反应。

该检测基于夹心ELISA方法，将样品添加到酶联免疫吸附试验（ELISA）带材板上，该带材板上预先涂有捕获的细胞因子特异性抗体。捕获的细胞因子通过添加生物素化的检测抗体来检测，然后添加链霉亲和素-辣根过氧化物酶，该酶与生物素化的抗体结合。添加显色酶底物3,3 ‘,5,5 ’四甲基联苯胺（TMB）可得到着色产物，其强度与样品中细胞因子的浓度成正比。细胞因子的浓度是通过与平行分析的细胞因子标准品的连续稀释进行比较来确定的。

Subtype
Complete Kits
 
Cell Type
B Cells, Cardiomyocytes, PSC-Derived, Dendritic Cells, Endothelial Cells, Granulocytes and Subsets, Macrophages, Mouse Embryonic Fibroblasts, Myogenic Stem and Progenitor Cells, NK Cells, T Cells
 
Species
Mouse
 
Application
ELISA
 
Area of Interest
Immunology, Stem Cell Biology
 

查看更多 显示更少

Data Figures

Representative Standard Curve

• Reportable range: 15.6 - 2000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 5 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC international standard 88/532.
• Recovery: A mid-curve recovery of 84 - 86% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 2.4% (CV). The Inter-assay precision of this assay is 3.7% (CV).

* National Institute of Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Mouse TNF-alpha ELISA Kit
Catalog #
02030, 02031
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
Mouse TNF-alpha ELISA Kit
Catalog #
02030, 02031
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
Mouse TNF-alpha ELISA Kit
Catalog #
02030, 02031
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
Mouse TNF-alpha ELISA Kit
Catalog #
02030, 02031
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
Mouse TNF-alpha ELISA Kit
Catalog #
02030, 02031
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
Mouse TNF-alpha ELISA Kit
Catalog #
02030, 02031
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Product Name
Mouse TNF-alpha ELISA Kit
Catalog #
02030, 02031
Lot #
All
Language
English
Document Type
Safety Data Sheet 7
Product Name
Mouse TNF-alpha ELISA Kit
Catalog #
02030, 02031
Lot #
All
Language
English
Document Type
Safety Data Sheet 8
Product Name
Mouse TNF-alpha ELISA Kit
Catalog #
02030, 02031
Lot #
All
Language
English
Document Type
Safety Data Sheet 9
Product Name
Mouse TNF-alpha ELISA Kit
Catalog #
02030, 02031
Lot #
All
Language
English
Document Type
Safety Data Sheet 10
Product Name
Mouse TNF-alpha ELISA Kit
Catalog #
02030, 02031
Lot #
All
Language
English

Resources and Publications

Educational Materials (2)

ELISA Kits for Immunology Research
Brochure
ELISA Kits for Immunology Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

更多信息

更多信息
Species Mouse
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
版权 © 2025 STEMCELL Technologies 技术有限公司。保留所有权利。