产品号 #60029_C
Rat monoclonal IgG2b antibody against mouse CD4
概述
The RM4-4 antibody reacts with mouse CD4, an ~55 kDa single-chain type 1 transmembrane glycoprotein and member of the immunoglobin (Ig) superfamily; CD4 contains four extracellular Ig-like domains. CD4 is expressed at relatively high levels by most thymocytes and a subpopulation of T cells (T-helper/inducer cells), and at lower levels on dendritic cells. In the mouse, CD4 is not expressed by monocytes/macrophages. CD4 binds to a non-polymorphic region of MHC II and acts as a co-receptor to the T cell receptor (TCR) in MHC II-restricted antigen recognition by enhancing the avidity of the association between the TCR and MHC II-antigen complex. CD4 also functions to amplify signals from the TCR to the cytoplasm through the interaction of its intracellular domain with cytoplasmic tyrosine kinases such as Lck. Binding of the RM4-4 antibody does not block binding of antibody clones RM4-5 or GK1.5.
Subtype
Primary Antibodies
Target Antigen
CD4
Alternative Names
L3T4, T4
Reactive Species
Mouse
Conjugation
APC, Biotin, FITC, PE, Unconjugated
Host Species
Rat
Cell Type
T Cells, T Cells, CD4+, T Cells, Regulatory
Species
Mouse
Application
Flow Cytometry, Functional Assay
Area of Interest
Immunology
Clone
RM4-4
Gene ID
12504
Isotype
IgG2b, kappa
Data Figures
Figure 1. Data for Unconjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-4, followed by a mouse anti-rat IgG2b antibody, FITC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC (solid line histogram).
Figure 2. Data for APC-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-4, APC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) (solid line histogram).
Figure 3. Data for Biotin-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-4, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Biotin (Catalog #60077BT), followed by SAV APC (solid line histogram).
Figure 4. Data for FITC-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-4, FITC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) (solid line histogram).
Figure 5. Data for PE-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-4, PE (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, PE (Catalog #60077PE) (solid line histogram).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Mouse CD4 Antibody, Clone RM4-4, APC Catalog #
60029AZ, 60029AZ.1 Lot #
All Language
English Document Type
Product Information Sheet 1 Product Name
Anti-Mouse CD4 Antibody, Clone RM4-4, Biotin Catalog #
60029BT.1, 60029BT Lot #
All Language
English Document Type
Product Information Sheet 2 Product Name
Anti-Mouse CD4 Antibody, Clone RM4-4, Biotin Catalog #
60029BT.1, 60029BT Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse CD4 Antibody, Clone RM4-4, FITC Catalog #
60029FI.1, 60029FI Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse CD4 Antibody, Clone RM4-4, PE Catalog #
60029PE.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse CD4 Antibody, Clone RM4-4, APC Catalog #
60029AZ, 60029AZ.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse CD4 Antibody, Clone RM4-4, Biotin Catalog #
60029BT.1, 60029BT Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse CD4 Antibody, Clone RM4-4, FITC Catalog #
60029FI.1, 60029FI Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse CD4 Antibody, Clone RM4-4, PE Catalog #
60029PE.1 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Resources and Publications
Educational Materials (4)
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