产品号 #78017_C
Granulocyte-macrophage colony-stimulating factor
概述
Granulocyte-macrophage colony-stimulating factor (GM-CSF) promotes the proliferation and differentiation of hematopoietic progenitor cells and the generation of neutrophils, eosinophils, and macrophages. In synergy with other cytokines such as stem cell factor, IL-3, erythropoietin, and thrombopoietin, it also stimulates erythroid and megakaryocyte progenitor cells (Barreda et al.). GM-CSF was first purified from the culture of mouse lung tissue after lipopolysaccharide treatment. GM-CSF is produced by multiple cell types, including stromal cells, Paneth cells, macrophages, dendritic cells (DCs), endothelial cells, smooth muscle cells, fibroblasts, chondrocytes, and Th1 and Th17 cells (Francisco-Cruz et al.). The receptor for GM-CSF (GM-CSFR) is composed of two subunits: the cytokine-specific α subunit (GMRα; CD116) and the common subunit βc (CD131) shared with IL-3 and IL-5 receptors (Broughton et al.). GM-CSFR is expressed on hematopoietic cells, including progenitor cells and immune cells, as well as non-hematopoietic cells. GM-CSF is able to stimulate the development of DCs that ingest, process, and present antigens to the immune system (Francisco-Cruz et al.).
Subtype
Cytokines
Cell Type
Dendritic Cells, Hematopoietic Stem and Progenitor Cells, Monocytes, Myeloid Cells
Species
Mouse
Area of Interest
Immunology, Stem Cell Biology
Purity
≥ 95%
Data Figures
(A) The biological activity of Mouse Recombinant GM-CSF was tested by its ability to promote the proliferation of FDC-P1 cells. Cell proliferation was measured after 91 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 3 - 5 pg/mL.
(B) 1 μg of Mouse Recombinant GM-CSF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant GM-CSF has a predicted molecular mass of 14.3 kDa.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Mouse Recombinant GM-CSF (E. coli-expressed) Catalog #
78017, 78017.2, 78017.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Mouse Recombinant GM-CSF (E. coli-expressed) Catalog #
78017, 78017.2, 78017.1 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Hematopoietic Stem and Progenitor Cell Research
Workflow Stages for Myeloid Cell Research
Workflow Stages for Dendritic Cell Research
Resources and Publications
Educational Materials (3)
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