重组小鼠 GM-CSF(E. coli表达)

粒细胞-巨噬细胞集落刺激因子

产品号 #(选择产品)

产品号 #78017_C

粒细胞-巨噬细胞集落刺激因子

总览

粒细胞-巨噬细胞集落刺激因子 (GM-CSF) 促进造血祖细胞的增殖和分化,以及中性粒细胞、嗜酸性粒细胞和巨噬细胞的生成。它与干细胞因子、IL-3、促红细胞生成素和血小板生成素等其他细胞因子协同作用,还能刺激红系和巨核细胞祖细胞 (Barreda et al.)。GM-CSF 最初是从经脂多糖处理的小鼠肺组织培养物中纯化的。GM-CSF 由多种细胞类型产生,包括基质细胞、潘氏细胞、巨噬细胞、树突状细胞 (DC)、内皮细胞、平滑肌细胞、成纤维细胞、软骨细胞以及 Th1 和 Th17 细胞 (Francisco-Cruz et al.)。 GM-CSF 受体 (GM-CSFR) 由两个亚基组成:细胞因子特异性 α 亚基 (GMRα; CD116) 和与 IL-3 和 IL-5 受体共用的 βc 亚基 (CD131) (Broughton et al.)。GM-CSFR 在造血细胞(包括祖细胞和免疫细胞)以及非造血细胞中表达。GM-CSF 能够刺激树突状细胞(DC)的发育,而树突状细胞负责吞噬、加工并将抗原呈递给免疫系统 (Francisco-Cruz et al.)。

亚型
细胞因子
 
细胞类型
树突状细胞(DCs),造血干/祖细胞,单核细胞,髓系细胞
 
种属
小鼠
 
研究领域
免疫,干细胞生物学
 
纯度
≥ 95 %
 

Data Figures

(A) The biological activity of Mouse Recombinant GM-CSF was tested by its ability to promote the proliferation of FDC-P1 cells. Cell proliferation was measured after 91 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 3 - 5 pg/mL.
(B) 1 μg of Mouse Recombinant GM-CSF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant GM-CSF has a predicted molecular mass of 14.3 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78017, 78017.2, 78017.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78017, 78017.2, 78017.1
Lot #
All
Language
English

Resources and Publications

Educational Materials (3)

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Species Mouse
Purity ≥ 95%
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