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重组人 GM-CSF（E. coli表达）

粒细胞-巨噬细胞集落刺激因子
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产品号 #78015_C

粒细胞-巨噬细胞集落刺激因子

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总览

粒细胞-巨噬细胞集落刺激因子 (GM-CSF) 促进造血祖细胞的增殖和分化，以及中性粒细胞、嗜酸性粒细胞和巨噬细胞的生成。它与干细胞因子、IL-3、促红细胞生成素和血小板生成素等其他细胞因子协同作用，还能刺激红系和巨核细胞祖细胞的生成 (Barreda et al.)。GM-CSF 由多种细胞类型产生，包括基质细胞、潘氏细胞、巨噬细胞、树突状细胞 (DC)、内皮细胞、平滑肌细胞、成纤维细胞、软骨细胞以及 Th1 和 Th17 T 细胞 (Francisco-Cruz et al.)。粒细胞-巨噬细胞集落刺激因子 (GM-CSF) 受体 (GM-CSFR) 由两个亚基组成：细胞因子特异性 α 亚基 (GMRα; CD116) 和与 IL-3 和 IL-5 受体共用的 βc 亚基 (CD131) (Broughton et al.)。GM-CSFR 在造血细胞（包括祖细胞和免疫细胞）以及非造血细胞中表达。重组人 GM-CSF (rhGM-CSF) 可在体内促进粒细胞（中性粒细胞、嗜酸性粒细胞和嗜碱性粒细胞）和单核细胞系的髓系细胞生成。该药物已在动员造血祖细胞和治疗化疗引起的中性粒细胞减少症方面进行了测试。 GM-CSF 能够刺激 DC 的发育，而 DC 可以摄取、处理并向免疫系统呈递抗原（Francisco-Cruz et al.）。

亚型
细胞因子
 
细胞类型
树突状细胞（DCs），造血干/祖细胞，中胚层，PSC衍生，单核细胞，髓系细胞
 
种属
人
 
研究领域
免疫，干细胞生物学
 
纯度
≥ 95 %
 

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Data Figures

(A) The biological activity of Human Recombinant GM-CSF was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation was measured after 48 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 0.08 - 0.12 ng/mL.
(B) 1 μg of Human Recombinant GM-CSF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant GM-CSF has a predicted molecular mass of 14.6 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Human Recombinant GM-CSF (E. coli-expressed)
Catalog #
78015.1, 78015.2, 78015, 78015.3
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant GM-CSF (E. coli-expressed)
Catalog #
78015.1, 78015.2, 78015, 78015.3
Lot #
All
Language
English

Resources and Publications

Educational Materials (3)

Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Cytokines, Chemokines and Growth Factors
Brochure
Cytokines, Chemokines and Growth Factors
Human Immune Cytokines
Wallchart
Human Immune Cytokines

Publications (1)

Direct induction of haematoendothelial programs in human pluripotent stem cells by transcriptional regulators. I. Elcheva et al. Nature communications 2014 jul

Abstract

Advancing pluripotent stem cell technologies for modelling haematopoietic stem cell development and blood therapies requires identifying key regulators of haematopoietic commitment from human pluripotent stem cells (hPSCs). Here, by screening the effect of 27 candidate factors, we reveal two groups of transcriptional regulators capable of inducing distinct haematopoietic programs from hPSCs: pan-myeloid (ETV2 and GATA2) and erythro-megakaryocytic (GATA2 and TAL1). In both cases, these transcription factors directly convert hPSCs to endothelium, which subsequently transform into blood cells with pan-myeloid or erythro-megakaryocytic potential. These data demonstrate that two distinct genetic programs regulate the haematopoietic development from hPSCs and that both of these programs specify hPSCs directly to haemogenic endothelial cells. In addition, this study provides a novel method for the efficient induction of blood and endothelial cells from hPSCs via the overexpression of modified mRNA for the selected transcription factors.
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Species Human
Purity ≥ 95%
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