重组小鼠 G-CSF

粒细胞集落刺激因子

产品号 #(选择产品)

产品号 #78014_C

粒细胞集落刺激因子

总览

粒细胞集落刺激因子 (G-CSF) 是 CSF 糖蛋白家族的成员,其功能调节造血细胞的增殖、分化和功能。它是参与中性粒细胞生成和刺激造血祖细胞形成粒细胞集落的关键细胞因子 (Metcalf & Nicola)。G-CSF 可引起一系列效应,包括暂时降低 SDF-1 表达 (Petit et al.)、激活裂解 VCAM-1 的金属蛋白酶 (Levesque et al.) 以及从交感神经系统释放去甲肾上腺素 (Katayama et al.),从而导致造血干细胞从骨髓释放或动员到外周。 G-CSF受体在多种造血细胞中表达,包括髓系定向祖细胞、中性粒细胞、粒细胞和单核细胞。除造血细胞外,G-CSF 还在心肌细胞、神经元细胞、间皮细胞和内皮细胞中表达。小鼠G-CSF最初从 WEHI-3B 髓系单核细胞白血病细胞系培养物中纯化得到的,作为 WEHI-3B 和其他髓系白血病细胞系终末分化的诱导剂(Nicola et al.)。随后,研究人员从恒表达 G-CSF 的小鼠纤维肉瘤 NFSA 细胞提取的 mRNA 构建的 cDNA 文库中,在猴 COS 细胞中克隆得到了 G-CSF(Tsuchiya et al.)。 G-CSF 与其受体结合导致 JAK/STAT、MAPK、PI3K 和 AKT 信号转导通路的激活。

亚型
细胞因子
 
细胞类型
癌细胞及细胞系,造血干/祖细胞,神经元
 
种属
小鼠
 
研究领域
神经科学,干细胞生物学
 
纯度
≥ 95 %
 

Data Figures

(A) The biological activity of Mouse Recombinant G-CSF was tested by its ability to promote the proliferation of NFS-60 cells. Cell proliferation was measured after 69 hours of culture using a fluorometric assay method The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 8 - 12 pg/mL.
(B) 2 μg of Mouse Recombinant G-CSF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant G-CSF has a predicted molecular mass of 19.1 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78014, 78014.1, 78014.2
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78014, 78014.1, 78014.2
Lot #
All
Language
English

Applications

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Resources and Publications

Educational Materials (2)

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Species Mouse
Purity ≥ 95%
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