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小鼠IL-2酶联免疫吸附测定试剂盒

用于小鼠白细胞介素2的检测和测定
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产品号 #02022_C

用于小鼠白细胞介素2的检测和测定

产品组分包括

  • 小鼠IL-2酶联免疫吸附测定试剂盒，2板（目录#02022）
    • 小鼠白细胞介素2 (IL-2) ELISA板，2x
    • 小鼠IL-2标准品，0.5µg
    • 小鼠IL-2生物素化检测抗体，30µL
    • Sa-hrp, 30µl
    • 标准重组缓冲液B， 1ml
    • 洗涤缓冲液（20X）， 120 mL
    • ELISA稀释液，120 mL
    • SA-HRP稀释液，25ml
    • TMB底物，25ml
    • 停止液，25ml
    • 粘接板盖，6个
  • 小鼠IL-2酶联免疫吸附测定试剂盒，10板（目录#02023）
    • 小鼠IL-2酶联免疫吸附板，10x
    • 小鼠IL-2标准品，0.5µg
    • 小鼠IL-2生物素化检测抗体，130µL
    • Sa-hrp, 130µl
    • 标准重组缓冲液B， 1ml
    • 洗涤缓冲液（20倍），5 × 120毫升
    • ELISA稀释剂，3 × 120ml
    • SA-HRP稀释剂，120 mL
    • TMB底物，120 mL
    • 停止液，120ml
    • 胶粘剂板盖，30x
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产品说明书及文档
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概述

小鼠白细胞介素2 （IL-2）酶联免疫吸附测定试剂盒是专为定量检测和测量小鼠白细胞介素2在生物液体，如血清，血浆和细胞培养上清。IL-2主要由活化的CD4+和CD8+ T细胞分泌，但也由B细胞和树突状细胞少量产生。IL-2是T细胞发育的主要调节因子，参与T细胞的生长和扩增，以及调节性T细胞的发育。

该检测基于夹心ELISA方法，将样品添加到酶联免疫吸附试验（ELISA）带材板上，该带材板上预先涂有捕获的细胞因子特异性抗体。捕获的细胞因子通过添加生物素化的检测抗体来检测，然后添加链霉亲和素-辣根过氧化物酶，该酶与生物素化的抗体结合。添加显色酶底物3,3 ‘,5,5 ’四甲基联苯胺（TMB）可得到着色产物，其强度与样品中细胞因子的浓度成正比。细胞因子的浓度是通过与平行分析的细胞因子标准品的连续稀释进行比较来确定的。

Subtype
Complete Kits
 
Cell Type
B Cells, Dendritic Cells, T Cells, CD4+, T Cells, CD8+
 
Species
Mouse
 
Application
ELISA
 
Area of Interest
Immunology
 

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Data Figures

Representative Standard Curve

• Reportable range: 10 - 1000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 4 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC* international standard 93/566.
• Recovery: A mid-curve recovery of 106 - 114% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 3.1% (CV). The inter-assay precision of this assay is 6.7% (CV).

*National Institute of Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Mouse IL-2 ELISA Kit
Catalog #
02023, 02022
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
Mouse IL-2 ELISA Kit
Catalog #
02023, 02022
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
Mouse IL-2 ELISA Kit
Catalog #
02023, 02022
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
Mouse IL-2 ELISA Kit
Catalog #
02023, 02022
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
Mouse IL-2 ELISA Kit
Catalog #
02023, 02022
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
Mouse IL-2 ELISA Kit
Catalog #
02023, 02022
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Product Name
Mouse IL-2 ELISA Kit
Catalog #
02023, 02022
Lot #
All
Language
English
Document Type
Safety Data Sheet 7
Product Name
Mouse IL-2 ELISA Kit
Catalog #
02023, 02022
Lot #
All
Language
English
Document Type
Safety Data Sheet 8
Product Name
Mouse IL-2 ELISA Kit
Catalog #
02023, 02022
Lot #
All
Language
English
Document Type
Safety Data Sheet 9
Product Name
Mouse IL-2 ELISA Kit
Catalog #
02023, 02022
Lot #
All
Language
English
Document Type
Safety Data Sheet 10
Product Name
Mouse IL-2 ELISA Kit
Catalog #
02023, 02022
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (2)

ELISA Kits for Immunology Research
Brochure
ELISA Kits for Immunology Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

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Species Mouse
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