小鼠IL-12 (p70)酶联免疫吸附测定试剂盒

用于小鼠白细胞介素12p70亚基的检测和测定

产品号 #(选择产品)

产品号 #02026_C

用于小鼠白细胞介素12p70亚基的检测和测定

产品组分包括

  • 小鼠IL-12 (p70)酶联免疫吸附测定试剂盒,2板(目录#02026)
    • 小鼠IL-12 (p70)酶联免疫吸附试验板,2个
    • 小鼠IL-12和IL-12/-23标准品,1瓶
    • 小鼠IL-12 (p70)生物素化检测抗体,30µL
    • Sa-hrp, 30µl
    • 标准重组缓冲液B, 1ml
    • 洗涤缓冲液(20X), 120 mL
    • ELISA稀释液,120 mL
    • SA-HRP稀释液,25ml
    • TMB底物,25ml
    • 停止液,25ml
    • 粘接板盖,6个
  • 小鼠IL-12 (p70)酶联免疫吸附测定试剂盒,10板(目录#02027)
    • 小鼠IL-12 (p70)酶联免疫吸附试验板,10x
    • 小鼠IL-12和IL-12/-23标准品,1瓶
    • 小鼠IL-12 (p70)生物素化检测抗体,130µL
    • Sa-hrp, 130µl
    • 标准重组缓冲液B, 1ml
    • 洗涤缓冲液(20倍),5 × 120毫升
    • ELISA稀释剂,3 × 120ml
    • SA-HRP稀释剂,120 mL
    • TMB底物,120 mL
    • 停止液,120ml
    • 胶粘剂板盖,30x

概述

小鼠白细胞介素12 (IL-12) p70酶联免疫吸附测定试剂盒是专为定量检测和测量小鼠白细胞介素12 (p70)在生物液体,如血清,血浆和细胞培养上清。IL-12 (p70)是由p35和p40亚基组成的异二聚体细胞因子;p40亚基与IL-23共享。IL-12由单核细胞、巨噬细胞、树突状细胞、中性粒细胞和B细胞产生。IL-12是细胞因子合成的调节因子,尤其是IFN-γ,促进T细胞和NK细胞的增殖,刺激CD4+和CD8+ T细胞的分化。

该检测基于夹心ELISA方法,将样品添加到酶联免疫吸附试验(ELISA)带材板上,该带材板上预先涂有捕获的细胞因子特异性抗体。捕获的细胞因子通过添加生物素化的检测抗体来检测,然后添加链霉亲和素-辣根过氧化物酶,该酶与生物素化的抗体结合。添加显色酶底物3,3 ‘,5,5 ’四甲基联苯胺(TMB)可得到着色产物,其强度与样品中细胞因子的浓度成正比。细胞因子的浓度是通过与平行分析的细胞因子标准品的连续稀释进行比较来确定的。

Subtype
Complete Kits
 
Cell Type
B Cells, Dendritic Cells, Granulocytes and Subsets, Macrophages, Monocytes, T Cells
 
Species
Mouse
 
Application
ELISA
 
Area of Interest
Immunology
 

Data Figures

Representative Standard Curve

• Reportable range: 10 - 3160 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 6 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: No international standard exists for calibration.
• Recovery: A mid-curve recovery of 85 - 121% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 3.7% (CV). The inter-assay precision of this assay is 6.8% (CV).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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02026, 02027
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English
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Safety Data Sheet 1
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Safety Data Sheet 2
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Safety Data Sheet 8
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Safety Data Sheet 9
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02026, 02027
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All
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English

Resources and Publications

Educational Materials (2)

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Species Mouse
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