The Mouse Interleukin 12 (IL-12) p70 ELISA Kit is designed for the quantitative detection and measurement of mouse IL-12 (p70) in biological fluids such as serum, plasma, and cell culture supernatants. IL-12 (p70) is a heterodimeric cytokine composed of p35 and p40 subunits; the p40 subunit is shared with IL-23. IL-12 is produced by monocytes, macrophages, dendritic cells, neutrophils, and B cells. IL-12 is a regulator of cytokine synthesis, especially IFN-γ, enhances the proliferation of T and NK cells, and stimulates the differentiation of CD4+ and CD8+ T cells.
The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.
The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.
Data Figures
Representative Standard Curve
• Reportable range: 10 - 3160 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 6 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: No international standard exists for calibration.
• Recovery: A mid-curve recovery of 85 - 121% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 3.7% (CV). The inter-assay precision of this assay is 6.8% (CV).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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Product Information Sheet
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Mouse IL-12 (p70) ELISA Kit
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02026, 02027
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All
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English
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Safety Data Sheet 1
Product Name
Mouse IL-12 (p70) ELISA Kit
Catalog #
02026, 02027
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All
Language
English
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Safety Data Sheet 2
Product Name
Mouse IL-12 (p70) ELISA Kit
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02026, 02027
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All
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English
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Safety Data Sheet 3
Product Name
Mouse IL-12 (p70) ELISA Kit
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02026, 02027
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All
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English
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Safety Data Sheet 4
Product Name
Mouse IL-12 (p70) ELISA Kit
Catalog #
02026, 02027
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All
Language
English
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Safety Data Sheet 5
Product Name
Mouse IL-12 (p70) ELISA Kit
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02026, 02027
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All
Language
English
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Safety Data Sheet 6
Product Name
Mouse IL-12 (p70) ELISA Kit
Catalog #
02026, 02027
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All
Language
English
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Safety Data Sheet 7
Product Name
Mouse IL-12 (p70) ELISA Kit
Catalog #
02026, 02027
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All
Language
English
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Safety Data Sheet 8
Product Name
Mouse IL-12 (p70) ELISA Kit
Catalog #
02026, 02027
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All
Language
English
Document Type
Safety Data Sheet 9
Product Name
Mouse IL-12 (p70) ELISA Kit
Catalog #
02026, 02027
Lot #
All
Language
English
Document Type
Safety Data Sheet 10
Product Name
Mouse IL-12 (p70) ELISA Kit
Catalog #
02026, 02027
Lot #
All
Language
English
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for B Cell Research
Workflow Stages for Dendritic Cell Research
Workflow Stages for Granulocyte Research
Workflow Stages for Monocyte Research
Resources and Publications
Educational Materials (2)
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