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小鼠IgG3，卡帕同型控制抗体，克隆MG3- 35

小鼠单克隆IgG3， kappa同型控制抗体

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产品号 #（选择产品）

产品号 #60073_C

小鼠单克隆IgG3， kappa同型控制抗体

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总览

实验数据

产品说明书及文档

概述

MG3-35抗体（IgG3, kappa）适合作为同型匹配对照抗体在几种应用中用于估计抗原特异性抗体的非特异性结合程度。理想情况下，同型对照应与所使用的特异性抗体具有相同的重链（IgA、IgD、IgE、IgG或IgM）和轻链（kappa或lambda）亚类。如果使用偶联抗体，则应选择与相同分子（如荧光染料）偶联的同型对照物。使用适当的同型对照有助于确认抗原特异性抗体的特异性，并指出与Fc受体或其他细胞成分结合可能导致的非特异性结合。

该抗体克隆已被证实可作为同型对照抗体，用于流式细胞术和免疫荧光显微镜应用（表面和细胞内染色）中评估与细胞的非特异性结合。

Subtype
Isotype Controls

Target Antigen
IgG3 Isotype Control

Alternative Names
Not applicable

Conjugation
Alexa Fluor 488, Biotin, PE, Unconjugated

Host Species
Mouse

Cell Type
Other

Species
Human, Mouse, Non-Human Primate, Other, Rat

Application
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting

Area of Interest
Immunology

Clone
MG3-35

Gene ID
Not applicable

Isotype
IgG3, kappa

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Data Figures

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of human iPS cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, followed by a goat anti-mouse IgG antibody, FITC (solid line histogram). Filled histogram shows labeling with a mouse IgG3, kappa positive control antibody (Anti-Human SSEA-4 Antibody, Clone MC-813-70, Catalog #60062) followed by a goat anti-mouse IgG antibody, FITC. (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Human SSEA-4 Antibody, Clone MC-813-70 (Catalog #60062), followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35.

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human iPS cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, Alexa Fluor® 488 (solid line histogram). Filled histogram shows labeling with a mouse IgG3, kappa positive control antibody (Anti-Human SSEA-4 Antibody, Clone MC-813-70, Alexa Fluor® 488, Catalog #60062AD). B) Human ES cells were cultured in mTeSR™1 on Corning Matrigel®-coated glass slides, then fixed and stained with Anti-Human SSEA-4 Antibody, Clone MC-813-70, Alexa Fluor® 488. Inset shows cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, Alexa Fluor® 488.

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of human ES cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, Biotin followed by streptavidin (SAV) APC (solid line histogram). Filled histogram shows labeling with a mouse IgG3, kappa positive control antibody (Anti-Human SSEA-4 Antibody, Clone MC-813-70, Biotin, Catalog #60062BT) followed by SAV APC.

Figure 4. Data for FITC-Conjugated

Flow cytometry analysis of human ES cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, FITC (solid line histogram). Filled histogram shows labeling with a mouse IgG3, kappa positive control antibody (Anti-Human SSEA-4 Antibody, Clone MC-813-70, FITC, Catalog #60062FI).

Figure 5. Data for PE-Conjugated

(A) Flow cytometry analysis of human iPS cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG3, kappa positive control antibody (Anti-Human SSEA-4 Antibody, Clone MC-813-70, PE, Catalog #60062PE). (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Human SSEA-4 Antibody, Clone MC-813-70, PE (Catalog #60062PE). Inset shows cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35.