产品号 #60070_C
小鼠单克隆IgG1， kappa同型控制抗体
概述
MOPC-21抗体（IgG1, kappa）适合作为同型匹配对照抗体在多种应用中用于估计抗原特异性抗体的非特异性结合程度。理想情况下，同型对照应与所使用的特异性抗体具有相同的重链（IgA、IgD、IgE、IgG或IgM）和轻链（kappa或lambda）亚类。如果使用偶联抗体，则应选择与相同分子（如荧光染料）偶联的同型对照物。使用适当的同型对照有助于确认抗原特异性抗体的特异性，并指出与Fc受体或其他细胞成分结合可能导致的非特异性结合。
该抗体克隆已被证实可作为同型对照抗体，用于流式细胞术和免疫荧光显微镜应用（表面和细胞内染色）中评估与细胞的非特异性结合。
Subtype
Isotype Controls
Target Antigen
IgG1 Isotype Control
Alternative Names
Not applicable
Conjugation
Alexa Fluor 488, APC, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
Other
Species
Human, Mouse, Non-Human Primate, Other, Rat
Application
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
MOPC-21
Gene ID
Not applicable
Isotype
IgG1, kappa
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (2)
Publications (1)
Trend of telomerase activity change during human iPSC self-renewal and differentiation revealed by a quartz crystal microbalance based assay. Scientific reports 2014 NOV
Abstract
Telomerase plays an important role in governing the life span of cells for its capacity to extend telomeres. As high activity of telomerase has been found in stem cells and cancer cells specifically, various methods have been developed for the evaluation of telomerase activity. To overcome the time-consuming procedures and complicated manipulations of existing methods, we developed a novel method named Telomeric Repeat Elongation Assay based on Quartz crystal microbalance (TREAQ) to monitor telomerase activity during the self-renewal and differentiation of human induced pluripotent stem cells (hiPSCs). TREAQ results indicated hiPSCs possess invariable telomerase activity for 11 passages on Matrigel and a steady decline of telomerase activity when differentiated for different periods, which is confirmed with existing golden standard method. The pluripotency of hiPSCs during differentiation could be estimated through monitoring telomerase activity and compared with the expression levels of markers of pluripotency gene via quantitative real time PCR. Regular assessment for factors associated with pluripotency or stemness was expensive and requires excessive sample consuming, thus TREAQ could be a promising alternative technology for routine monitoring of telomerase activity and estimate the pluripotency of stem cells.
更多信息
| Species | Human, Mouse, Non-Human Primate, Other, Rat |
|---|---|
| Clone | MOPC-21 |
| Gene Id | Not applicable |
| Alternative Names | Not applicable |
| Isotype | IgG1, kappa |
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Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
