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小鼠 IgG2b，kappa 同型对照抗体（克隆 MPC-11）

小鼠单克隆IgG2b， kappa同型对照抗体
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产品号 #60072_C

小鼠单克隆IgG2b， kappa同型对照抗体

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总览
实验数据
产品说明书及文档
应用领域
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总览

MPC-11 抗体 (IgG2b, kappa) 适合在多种应用中用作同型对照抗体，以评估抗原特异性抗体的非特异性结合程度。理想情况下，同型对照应与所用特异性抗体具有相同的重链亚类（IgA、IgD、IgE、IgG 或 IgM）和轻链亚类（kappa 或 lambda）。如果使用偶联抗体，则应选择与相同分子（例如荧光染料）偶联的同型对照。使用合适的同型对照有助于确认抗原特异性抗体的特异性，并指示可能由与 Fc 受体或其他细胞成分结合引起的非特异性结合。

该抗体克隆已验证可用作同型对照抗体，用于评估流式细胞术和免疫荧光显微镜应用（表面和细胞内染色）等应用中的细胞非特异性结合。

亚型
同型对照抗体
 
靶抗原
IgG2b Isotype Control
 
别名
Not applicable
 
偶联
Alexa Fluor 488，APC，Biotin 或 生物素，FITC，PE，PerCP-Cyanine5.5，未偶联的
 
宿主物种
小鼠
 
细胞类型
其它细胞系
 
种属
人，小鼠，非人灵长类，其它细胞系，大鼠
 
应用
ELISAs，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹
 
研究领域
免疫
 
克隆
MPC-11
 
基因编号
Not applicable
 
同种型
IgG2b，kappa
 

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Data Figures

Flow Cytometry Analysis of Human PBMCs

Figure 1. Flow Cytometry Analysis of Human PBMCs

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC11, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7; Catalog #60008), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC. (B) Human induced pluripotent stem (iPS) cells were cultured in TeSR™-E8™ (Catalog #05940) on glass coverslips coated with Vitronectin XF™ (Catalog #07180), then fixed and labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, followed by goat anti-mouse IgG, FITC (Inset), or with a positive control antibody of the same isotype, Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20 (Catalog #60093), followed by goat anti-mouse IgG, FITC (green).

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Alexa Fluor® 488 (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7, Alexa Fluor® 488, Catalog #60008AD). (B) Human embryonic stem (ES) cells were cultured with TeSR™-E8™ (Catalog #05940) on glass coverslips coated with Vitronectin XF™ (Catalog #07180), then fixed and stained with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Alexa Fluor® 488 (inset) or with a positive control antibody of the same isotype, Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, Alexa Fluor® 488 (Catalog #60093AD, green).

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of human buffy coat nucleated cells labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, APC (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7, APC, Catalog #60008AZ).

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Biotin followed by streptavidin (SAV) APC (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7, Biotin, Catalog #60008BT) followed by SAV APC.

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

Flow cytometry analysis of human buffy coat nucleated cells labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, FITC (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7, FITC, Catalog #60008FI).

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7, PE, Catalog #60008PE). (B) Human embryonic stem (ES) cells were cultured with TeSR™-E8™ (Catalog #05940) on glass coverslips coated with Vitronectin XF™ (Catalog #07180), then fixed and stained with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PE (inset) or with a positive control antibody of the same isotype, Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, PE (Catalog #60093PE).

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PerCP-Cy5.5 (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7, PerCP-Cy5.5, Catalog #60008PS).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Alexa Fluor® 488
Catalog #
60072AD
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, APC
Catalog #
60072AZ, 60072AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Biotin
Catalog #
60072BT.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, FITC
Catalog #
60072FI, 60072FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PE
Catalog #
60072PE, 60072PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11
Catalog #
60072.1, 60072
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Alexa Fluor® 488
Catalog #
60072AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, APC
Catalog #
60072AZ, 60072AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Biotin
Catalog #
60072BT.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, FITC
Catalog #
60072FI, 60072FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PE
Catalog #
60072PE, 60072PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11
Catalog #
60072.1, 60072
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (2)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

更多信息

更多信息
Species Human, Mouse, Non-Human Primate, Other, Rat
Clone MPC-11
Gene Id Not applicable
Alternative Names Not applicable
Isotype IgG2b, kappa
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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