产品号 #60072_C
Mouse monoclonal IgG2b, kappa isotype control antibody
概述
The MPC-11 antibody (IgG2b, kappa) is suitable for use as an isotype-matched control antibody in several applications to estimate the degree of non-specific binding by an antigen-specific antibody. Ideally, the isotype control should have the same subclass of heavy chain (IgA, IgD, IgE, IgG, or IgM) and light chain (kappa or lambda) as the specific antibody being used. If a conjugated antibody is used, an isotype control conjugated to the same molecule (e.g. fluorochrome) should be chosen. The use of an appropriate isotype control helps confirm the specificity of the antigen-specific antibody, and indicates non-specific binding that may result from binding to Fc receptors or other cell components.
This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining).
Subtype
Isotype Controls
Target Antigen
IgG2b Isotype Control
Alternative Names
Not applicable
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Mouse
Cell Type
Other
Species
Human, Mouse, Non-Human Primate, Other, Rat
Application
ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
MPC-11
Gene ID
Not applicable
Isotype
IgG2b, kappa
Data Figures
Figure 1. Flow Cytometry Analysis of Human PBMCs
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC11, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7; Catalog #60008), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC. (B) Human induced pluripotent stem (iPS) cells were cultured in TeSR™-E8™ (Catalog #05940) on glass coverslips coated with Vitronectin XF™ (Catalog #07180), then fixed and labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, followed by goat anti-mouse IgG, FITC (Inset), or with a positive control antibody of the same isotype, Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20 (Catalog #60093), followed by goat anti-mouse IgG, FITC (green).
Figure 2. Data for Alexa Fluor® 488-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Alexa Fluor® 488 (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7, Alexa Fluor® 488, Catalog #60008AD). (B) Human embryonic stem (ES) cells were cultured with TeSR™-E8™ (Catalog #05940) on glass coverslips coated with Vitronectin XF™ (Catalog #07180), then fixed and stained with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Alexa Fluor® 488 (inset) or with a positive control antibody of the same isotype, Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, Alexa Fluor® 488 (Catalog #60093AD, green).
Figure 3. Data for APC-Conjugated
Flow cytometry analysis of human buffy coat nucleated cells labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, APC (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7, APC, Catalog #60008AZ).
Figure 4. Data for Biotin-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Biotin followed by streptavidin (SAV) APC (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7, Biotin, Catalog #60008BT) followed by SAV APC.
Figure 5. Data for FITC-Conjugated
Flow cytometry analysis of human buffy coat nucleated cells labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, FITC (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7, FITC, Catalog #60008FI).
Figure 6. Data for PE-Conjugated
(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7, PE, Catalog #60008PE). (B) Human embryonic stem (ES) cells were cultured with TeSR™-E8™ (Catalog #05940) on glass coverslips coated with Vitronectin XF™ (Catalog #07180), then fixed and stained with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PE (inset) or with a positive control antibody of the same isotype, Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, PE (Catalog #60093PE).
Figure 7. Data for PerCP-Cy55-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PerCP-Cy5.5 (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7, PerCP-Cy5.5, Catalog #60008PS).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for CHO and Other Cell Line Development
Workflow Stages for Hybridoma Production
Resources and Publications
Educational Materials (2)
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Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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