The Mouse Interferon-gamma (IFN-γ) ELISA Kit is designed for the quantitative detection and measurement of mouse IFN-γ in biological fluids such as serum, plasma, and cell culture supernatants. IFN-γ is primarily secreted by T and NK cells, but is also produced by dendritic cells and macrophages in smaller amounts. IFN-γ is a pleiotropic cytokine that modulates a broad range of immunological functions including antimicrobial and antitumor activity, and increases the surface expression of MHC class I and II proteins.
The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate
3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.
Subtype Complete Kits
Cell Type Dendritic Cells, Macrophages, NK Cells, T Cells
Species Mouse
Area of Interest Immunology
Data Figures
Representative Standard Curve
• Reportable range: 3.2 - 1000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity. • Sensitivity: The limit of detection of this assay is 2 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard. • Accuracy: The analyte standard of this ELISA was calibrated against NIAID* international standard Gxg02-901-533. • Recovery: A mid-curve recovery of 93 - 111% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments. • Precision: The intra-assay precision of this assay is 4.4% (CV). The inter-assay precision of this assay is 6.3% (CV).
*National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892-6612, USA.
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