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小鼠ifn - γ酶联免疫吸附测定试剂盒

用于小鼠干扰素γ的检测和测量
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产品号 #02020_C

用于小鼠干扰素γ的检测和测量

产品组分包括

  • 小鼠ifn - γ酶联免疫吸附测定试剂盒，2板（目录#02020）
    • 小鼠IFN-γ ELISA板，2x
    • 小鼠IFN-γ标准品1瓶
    • 小鼠IFN-γ生物素化检测抗体，30µL
    • Sa-hrp, 30µl
    • 标准重构缓冲液C， 1ml
    • 洗涤缓冲液（20X）， 120 mL
    • ELISA稀释液，120 mL
    • SA-HRP稀释液，25ml
    • TMB底物，25ml
    • 停止液，25ml
    • 粘接板盖，6个
  • 小鼠ifn - γ酶联免疫吸附测定试剂盒，10板（目录#02021）
    • 小鼠IFN-γ ELISA板，10x
    • 小鼠IFN-γ标准品1瓶
    • 小鼠IFN-γ生物素化检测抗体，130µL
    • Sa-hrp, 130µl
    • 标准重构缓冲液C， 1ml
    • 洗涤缓冲液（20倍），5 × 120毫升
    • ELISA稀释剂，3 × 120ml
    • SA-HRP稀释剂，120 mL
    • TMB底物，120 mL
    • 停止液，120ml
    • 胶粘剂板盖，30x
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概述

小鼠干扰素γ (IFN-γ)酶联免疫吸附测定试剂盒是专为定量检测和测量小鼠IFN-γ在生物液体，如血清，血浆和细胞培养上清。IFN-γ主要由T细胞和NK细胞分泌，但树突状细胞和巨噬细胞也产生少量IFN-γ。IFN-γ是一种多效性细胞因子，调节多种免疫功能，包括抗菌和抗肿瘤活性，并增加MHC I类和II类蛋白的表面表达。

该检测基于夹心ELISA方法，将样品添加到酶联免疫吸附试验（ELISA）带材板上，该带材板上预先涂有捕获的细胞因子特异性抗体。捕获的细胞因子通过添加生物素化的检测抗体来检测，然后添加链霉亲和素-辣根过氧化物酶，该酶与生物素化的抗体结合。添加显色酶底物
3,3 ‘,5,5 ’四甲基联苯胺（TMB）产生的着色产物的强度与样品中细胞因子的浓度成正比。细胞因子的浓度是通过与平行分析的细胞因子标准品的连续稀释进行比较来确定的。

Subtype
Complete Kits
 
Cell Type
Dendritic Cells, Macrophages, NK Cells, T Cells
 
Species
Mouse
 
Area of Interest
Immunology
 

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Data Figures

Representative Standard Curve

• Reportable range: 3.2 - 1000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 2 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIAID* international standard Gxg02-901-533.
• Recovery: A mid-curve recovery of 93 - 111% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 4.4% (CV). The inter-assay precision of this assay is 6.3% (CV).

*National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892-6612, USA.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Mouse IFN-gamma ELISA Kit
Catalog #
02020, 02021
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
Mouse IFN-gamma ELISA Kit
Catalog #
02020, 02021
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
Mouse IFN-gamma ELISA Kit
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02020, 02021
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Language
English
Document Type
Safety Data Sheet 3
Product Name
Mouse IFN-gamma ELISA Kit
Catalog #
02020, 02021
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Language
English
Document Type
Safety Data Sheet 4
Product Name
Mouse IFN-gamma ELISA Kit
Catalog #
02020, 02021
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Language
English
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Safety Data Sheet 5
Product Name
Mouse IFN-gamma ELISA Kit
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02020, 02021
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Language
English
Document Type
Safety Data Sheet 6
Product Name
Mouse IFN-gamma ELISA Kit
Catalog #
02020, 02021
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Language
English
Document Type
Safety Data Sheet 7
Product Name
Mouse IFN-gamma ELISA Kit
Catalog #
02020, 02021
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Language
English
Document Type
Safety Data Sheet 8
Product Name
Mouse IFN-gamma ELISA Kit
Catalog #
02020, 02021
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Language
English
Document Type
Safety Data Sheet 9
Product Name
Mouse IFN-gamma ELISA Kit
Catalog #
02020, 02021
Lot #
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Language
English
Document Type
Safety Data Sheet 10
Product Name
Mouse IFN-gamma ELISA Kit
Catalog #
02020, 02021
Lot #
All
Language
English

Resources and Publications

Educational Materials (2)

ELISA Kits for Immunology Research
Brochure
ELISA Kits for Immunology Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

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Species Mouse
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