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ImmunoCult™小鼠T细胞激活试剂盒

小鼠T细胞激活和扩增试剂
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产品号 #（选择产品）

产品号 #100-1572_C

小鼠T细胞激活和扩增试剂

产品优势

  • 只需单个试剂盒即可高效地激活小鼠T细胞，并且可灵活地选择使用CD3/CD28或CD3/CD28/CD2进行激活。
  • 无需磁珠、饲养层细胞或抗原即可实现最佳激活。
  • 温和，可维持功能性T细胞的高存活率。
  • 高度稳定、过滤灭菌的可溶性试剂， 与常规T细胞生长培养基兼容。

产品组分包括

  • ImmunoCult™ Mouse T Cell Activator Component A, 1.0 mL
  • ImmunoCult™ Mouse T Cell Activator Component B, 1.0 mL
  • ImmunoCult™ Mouse T Cell Activator Component C, 1.0 mL
main product photo
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

ImmunoCult™小鼠T细胞激活试剂盒可温和地激活T细胞，从而保持T细胞的高存活率，激活后的T细胞可以在常规小鼠T细胞生长培养基中进一步扩增。该试剂盒由结合并交联CD3和CD28细胞表面配体的抗体复合物组成，还可以靶向CD2，从而为T细胞培养和激活提供所需的初级和共刺激信号。

使用所有3种试剂盒成分（A、B和C）可以在2-3天内实现最佳的T细胞激活。组分A含有用于必要抗原和共刺激激活信号的抗小鼠CD3和CD28抗体。组分B含有用于选择性靶向的抗小鼠CD2抗体。组分C有助于交联组分A和B中的抗体。所有试剂均高度稳定、经过过滤灭菌且可溶，无需耗时的培养板包被步骤，同时允许无缝衔接至当前的工作流程中。

为了有效分离小鼠T细胞，ImmunoCult™小鼠T细胞激活剂试剂盒可以与EasySep™工作流程结合使用。它与EasySep™小鼠T细胞分选试剂盒（产品号 #19851）、EasySep™小鼠CD4+T细胞分选（产品号 #19852）、EasySep™小鼠CD8+T细胞分选试剂盒（产品号 #19853）、EasySep™小鼠Naive CD4+ T细胞分选试剂盒（产品号 #19765）、EasySep™ Release小鼠CD4+CD304+调节性T细胞分选试剂盒（产品号 #100-1570）和EasySepTM小鼠CD4正选试剂盒II（产品号 #18952；肺组织）等试剂盒兼容。

包含
Anti-mouse CD3 monospecific antibody Anti-mouse CD28 monospecific antibody  Anti-mouse CD2 monospecific antibody Antibody crosslinking complex
 
亚型
完整试剂盒
 
细胞类型
T 细胞，T 细胞，CD4+，T 细胞，CD8+，T 细胞，调节性细胞
 
种属
小鼠
 
应用
激活，细胞培养，扩增
 
品牌
ImmunoCult
 
研究领域
免疫，细胞治疗开发
 

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Data Figures

Figure 1. ImmunoCult™ Mouse T Cell Activator Kit Supports High Viability of Activated T Cells

Figure 1. Activation of EasySep™-Isolated Mouse T Cells Stimulated with ImmunoCult™ Mouse T Cell Activator Kit

Mouse T cells were isolated using EasySep™ Mouse T Cell Isolation Kit, stimulated with ImmunoCult™ Mouse T Cell Activator Kit, and cultured in IMDM + FBS formulation. Following 3 days of culture, the mean ± SD frequency of CD25+ cells was 91.9 ± 5.1% (n = 11) or 99.9 ± 0.1% (n = 5), when stimulated with ImmunoCult™ Mouse CD3/CD28 T Cell Activator or ImmunoCult™ Mouse CD3/CD28/CD2 T Cell Activator, respectively. Stimulated mouse T cells maintained expression levels of CD25 throughout the 7-day culture period.

Figure 2. Robust Expansion of EasySep™-Isolated Mouse T Cells Can Be Achieved Following Stimulation with ImmunoCult™ Mouse T Cell Activator Kit

Figure 2. Robust Expansion of EasySep™-Isolated Mouse T Cells Can Be Achieved Following Stimulation with ImmunoCult™ Mouse T Cell Activator Kit

EasySep™-isolated mouse T cells were expanded with ImmunoCult™ Mouse T Cell Activator Kit in IMDM + FBS formulation over 7 days. The number of viable cells was assessed every 2 - 3 days, when fresh medium supplemented with IL-2 was added. No additional ImmunoCult™ Mouse T Cell Activator was added during the 7-day culture period. After 7 days in culture with ImmunoCult™ Mouse CD3/CD28 T Cell Activator or ImmunoCult™ Mouse CD3/CD28/CD2 T Cell Activator, stimulation resulted in a fold expansion of 23 ± 3.4 or 29.3 ± 4.8 (mean ± SEM, n = 6), respectively.

Figure 3. High Cell Proliferation is Observed in EasySep™-Isolated T cells After Stimulation with ImmunoCult™ Mouse T Cell Activator

Figure 3. High Cell Proliferation Is Observed in EasySep™-Isolated T cells After Stimulation with ImmunoCult™ Mouse T Cell Activator

EasySep™-isolated mouse T cells were labeled with CFDA-SE (Catalog #75003), stimulated with ImmunoCult™ Mouse T Cell Activator Kit, and cultured in IMDM + FBS formulation. On Day 3, cells were harvested, stained with anti-mouse CD4 and CD8a antibodies, then measured by flow cytometry. Shown are CFDA-SE-labeled mouse T cells, gated on viable (A) CD4+ or (B) CD8a+ cells, cultured with no activator (top panel), with ImmunoCult™ Mouse CD3/CD28 T Cell Activator (middle panel), or with ImmunoCult™ Mouse CD3/CD28/CD2 T Cell Activator (bottom panel). Due to cell proliferation, the intensity of CFDA-SE signal is reduced by 50% for each cell division.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
ImmunoCult™ Mouse T Cell Activator Kit
Catalog #
100-1572
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
ImmunoCult™ Mouse T Cell Activator Kit
Catalog #
100-1572
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
ImmunoCult™ Mouse T Cell Activator Kit
Catalog #
100-1572
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
ImmunoCult™ Mouse T Cell Activator Kit
Catalog #
100-1572
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (2)

Mouse T Cell Research
Brochure
Mouse T Cell Research
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research

更多信息

更多信息
Species Mouse
Contains Anti-mouse CD3 monospecific antibody Anti-mouse CD28 monospecific antibody Anti-mouse CD2 monospecific antibody Antibody crosslinking complex
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