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ImmunoCult™ 小鼠Th1分化添加剂

小鼠naïve CD4+ T细胞向Th1细胞分化的无血清培养补充
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产品号 #（选择产品）

产品号 #10953_C

小鼠naïve CD4+ T细胞向Th1细胞分化的无血清培养补充

产品优势

  • 为诱导从C57BL/6小鼠脾脏分选的初始CD4+T细胞向Th1分化而优化；
  • 作为100X浓缩物供应；解冻和混匀后，管内容物可直接添加到培养基中
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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

ImmunoCult™小鼠Th1分化补充剂含有重组小鼠细胞因子和阻断单克隆抗体的组合，用于促进小鼠naïve CD4+ T细胞向Th1细胞的分化。该补充剂与含有胎牛血清和其他添加剂的RPMI 1640培养基（产品号#36750）以及抗小鼠CD3和抗小鼠CD28单克隆抗体作为活化剂体系搭配使用。

本产品推荐用于：

•研究小鼠naïve T细胞分化和Th1功能的调节

•开发将naïve T细胞扩增以及向Th1亚群极化的工艺流程

包含
• Recombinant mouse interleukin 2 (IL-2) • Recombinant mouse interleukin 12 (IL-12) • Rat anti-mouse interleukin 4 (anti-IL-4)
 
亚型
添加剂
 
细胞类型
T 细胞，T 细胞，CD4+
 
种属
小鼠
 
应用
细胞培养，分化，扩增
 
品牌
ImmunoCult
 
研究领域
免疫
 

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Data Figures

Figure 1. ImmunoCult™ Mouse Th1 Differentiation Supplement Produces CD4+IFNγ+T-Bet+ Cells Under Th1 Polarizing Conditions

Naïve CD4+ T cells were isolated from mouse splenocytes using the EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), activated with plate-bound anti-CD3 and soluble anti-CD28 and cultured in medium alone (Non-polarized cultures), or medium supplemented with ImmunoCult™ Mouse Th1 Differentiation Supplement (Polarized cultures ) for 5 days. Cells were subsequently stimulated with PMA/Ionomycin, in conjunction with brefeldin A treatment, followed by staining with anti-CD4, anti-IFNγ, anti-T-bet and a viability dye and analyzed by flow cytometry. Shown is the expression of IFNγ and T-bet, back-gated on viable, CD4+ cells from non-polarized (A) or polarized cultures (B). The mean proportion of CD4+IFNγ+T-Bet+ cells is significantly higher in cells cultured in ImmunoCult™ Mouse Th1 Differentiation Supplement (44 ± 6%) compared to non-polarized cells (2 ± 1%) (p < 0.001; n = 13). Data from experimental groups were compared using a paired T-test.

Figure 2. ImmunoCult™ Mouse Th1 Differentiation Supplement Expands Total Nucleated Cells Under Th1 Polarizing Conditions

Naïve CD4+ T cells were isolated from mouse splenocytes using the EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), activated with plate-bound anti-CD3 and soluble anti-CD28 and cultured in medium alone, or medium containing ImmunoCult™ Mouse Th1 Differentiation Supplement for 5 days. A 4.5-fold expansion of total nuclear cells (TNC) ensues in cultures polarized with ImmunoCult Mouse Th1 Differentiation Supplement (purple column), which is significantly higher compared to non-polarized cultures (grey column). (Data represent the mean ± SEM, n = 11 - 20, *p < 0.05. Data from experimental groups were compared using a paired T-test).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
ImmunoCult™ Mouse Th1 Differentiation Supplement
Catalog #
10953
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ImmunoCult™ Mouse Th1 Differentiation Supplement
Catalog #
10953
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Species Mouse
Contains • Recombinant mouse interleukin 2 (IL-2) • Recombinant mouse interleukin 12 (IL-12) • Rat anti-mouse interleukin 4 (anti-IL-4)
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