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人iPSC衍生的视网膜色素上皮细胞

人诱导多能干细胞系分化的冻存的视网膜色素上皮细胞
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产品号 #200-0912_C

人诱导多能干细胞系分化的冻存的视网膜色素上皮细胞

产品优势

  • 通过经充分鉴定的hiPSC对照细胞系分化获得高品质RPE细胞，加速您的研究进程
  • 5周即可获得具有极化特性和色素沉着功能的成熟细胞，且具备光感受器外节吞噬功能
  • STEMdiff™-XF RPE成熟培养基支持RPE细胞长期维持培养
  • 灵活用于视网膜疾病建模、治疗药物发现和再生医学等应用
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Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. Vitronectin XF™
    Vitronectin XF™

    Defined, xeno-free matrix that supports the growth and differentiation of human pluripotent stem cells under serum-free, feeder-free conditions

  2. STEMdiff™-ACF RPE Plating Supplement
    STEMdiff™-ACF RPE Plating Supplement

    Animal component-free supplement for improving survival and attachment of hPSC-derived retinal pigment epithelial cells

  3. STEMdiff™-XF RPE Maturation Medium
    STEMdiff™-XF RPE Maturation Medium

    Serum-free and xeno-free medium for generating fully functional and mature hPSC-derived retinal pigment epithelium

总览
实验数据
产品说明书及文档
相关产品
选择高品质、即用型人iPSC衍生视网膜色素上皮（RPE）细胞，助您轻松开启间视网膜研究。这些冻存的RPE细胞来源于经充分验证的健康对照人诱导多能干细胞（iPSC）系SCTi003-A或SCTi004-A。复苏后，使用STEMdiff™-XF RPE成熟培养基可长期培养并维持细胞成熟状态。培养5周后，细胞呈现功能性成熟特征、RPE特征标志物高表达、色素沉着及多边形形态、极化结构形成和光感受器外节吞噬功能。RPE细胞采用无血清、无动物成分的STEMdiff™-ACF RPE分化试剂盒制备。为获得最佳细胞存活率和贴壁效果，建议解冻时使用STEMdiff™-XF RPE成熟培养基和STEMdiff™-ACF RPE贴壁添加物.。
本产品仅供研究使用 (RUO) ，已获得学术和商业使用授权。原始血样采集均通过机构审查委员会 (IRB) 或其他监管机构批准的知情同意书和方案进行，确保符合伦理规范。源细胞库的供体详细信息和细胞质量表征数据请参阅本页面图表。 更多详情请参考iPSC细胞系常见问题解答。
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Data Figures

Human iPSC-Derived Retinal Pigment Epithelial Cells Show Characteristic RPE Morphology

Figure 1. Human iPSC-Derived Retinal Pigment Epithelial Cells Show Characteristic RPE Morphology

Cryopreserved Human iPSC-Derived Retinal Pigment Epithelial (RPE) Cells were thawed and plated onto Corning® Matrigel®-coated plates at 150,000 cells/cm². RPE cells were maintained in STEMdiff™-XF RPE Maturation Medium supplemented with STEMdiff™-ACF RPE Plating Supplement for 7 days, followed by STEMdiff™-XF RPE Maturation Medium alone thereafter. The cells were incubated at 37°C and subsequently analyzed by brightfield microscopy at various time points. Representative microscopy images show that RPE cells display the expected morphology after more than 63 days of culture.

Human iPSC-Derived Retinal Pigment Epithelial (RPE) Cells Are Mature and Functional at Day 35.

Figure 2. Human iPSC-Derived Retinal Pigment Epithelial (RPE) Cells Are Mature and Functional at Day 35.

Human iPSC-Derived RPE Cells were thawed and cultured in STEMdiff™-XF RPE Maturation Medium for at least 35 days to demonstrate RPE maturity.

(A) The percentage of RPE cells, derived from two different iPSC lines, expressing PMEL17, CRALBP, EZRIN, and RPE65 was assessed by flow cytometry analysis. Data are reported as mean ± SEM (n = 4).

(B,C) Mature RPE cells exhibit high expression of CRALBP and display extensive tight junctions, as indicated by the localization of ZO-1 and BEST1 at cell boundaries. (D,E) Mature RPE cells are polarized, expressing EZRIN (3D projection) and proteins essential for the visual cycle, such as RPE65. These markers are visualized by fluorescence microscopy.

Human iPSC-Derived Retinal Pigment Epithelial Cells Display Key RPE Functionality

Figure 3. Human iPSC-Derived Retinal Pigment Epithelial Cells Display Key RPE Functionality

Human iPSC-Derived Retinal Pigment Epithelial (RPE) Cells were cultured on cell culture inserts in STEMdiff™-XF RPE Maturation Medium for 35 days. Apical and basal conditioned media were collected from mature RPE cells, and a sandwich ELISA was performed to quantify vascular endothelial growth factor (VEGF) and pigment epithelium-derived growth factor (PEDF) secretion.

(A) A cross-sectional schematic of the cell insert culture system illustrates the setup. Mature RPE cells, derived from two iPSC lines, secreted higher levels of (B) VEGF basally and (C) PEDF apically, demonstrating correct apicobasal polarity.

(D) Mature RPE cells also generated a strong barrier with high transepithelial resistance (TER). (E) Additionally, mature RPE cells were fed FITC-labeled bovine photoreceptor outer segments (POS) for 4 - 5 hours and efficiently internalized the bovine POS. Data are presented as mean ± SEM.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Human iPSC-Derived Retinal Pigment Epithelial Cells
Catalog #
100-2151, 200-0913, 200-0912, 100-2150
Lot #
All
Language
English

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Species Human
Cell And Tissue Source Pluripotent Stem Cells
Legal Statement: These iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) shall not be used or administered in (1) human subjects for human clinical use; (2) animals for veterinary use for therapeutic, diagnostic, or prophylactic purposes or (3) any subject in relation to clinical applications, cell therapy, transplantation, and/or regenerative medicines, without limiting the generality of the foregoing. These iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) may not be used for monetization or commercialization purposes, including without limitation, used to, or with the goal to, perform services or supply products or rights, including in the manufacture of cellular therapies or other therapeutics, for monetary gain or the generation of royalties, revenues, sales or other valuable consideration. For clarity, these iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) may not be used for screening compounds, antibodies, proteins or peptides, except for the purposes of target discovery, target validation, or assay development, provided such activities and the results of such activities are not further used for monetization or commercialization purposes. It may be possible to obtain a further license for the prohibited uses referred to in this Limited Use License. Please contact iPSCrequests@stemcell.com for more details. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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