产品号 #100-1364_C
提高hpsc源性视网膜色素上皮细胞存活和附着的动物无组分补充剂
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
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STEMdiff™-XF RPE Maturation MediumSerum-free and xeno-free medium for generating fully functional and mature hPSC-derived retinal pigment epithelium
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STEMdiff™-ACF RPE Differentiation KitAnimal component-free differentiation kit for the generation of immature retinal pigment epithelium from human pluripotent stem cells
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Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells
概述
通过添加STEMdiff™-ACF RPE镀补品，显著提高人类多能干细胞（hPSC）衍生的视网膜色素上皮（RPE）细胞在解离，解冻和复制后的存活率STEMdiff™-XF RPE成熟培养基.
本补充品旨在提高您的实验通量，生成hpsc衍生的RPE细胞STEMdiff™-ACF RPE分化试剂盒.
使用此补充作为一个完整的，优化的工作流程的一部分与以下兼容STEMdiff™RPE产品：
STEMdiff™-ACF RPE分化试剂盒
STEMdiff™-XF RPE成熟培养基
如果您打算将本产品用于商业或临床应用，请与我们联系。
Cell Type
Neural Cells, PSC-Derived, Pluripotent Stem Cells
Application
Cell Culture
Brand
STEMdiff
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience, Cell Therapy Development
Data Figures
Figure 1. Workflow for the Differentiation of hPSCs into Retinal Pigment Epithelium (RPE) with STEMdiff™ RPE Culture System
hPSC colonies, previously harvested and seeded as clumps, are put directly into the medium provided in STEMdiff™-ACF RPE Differentiation Kit. Seeding into STEMdiff™ RPE Differentiation Medium A induces cells toward immature RPE. A full medium change is performed on Day 1 with fresh STEMdiff™ RPE Differentiation Medium A and then on Day 2 with STEMdiff™ RPE Differentiation Medium B. On Days 4 and 6, medium changes are performed with fresh STEMdiff™ RPE Differentiation Medium C. On Days 7 and every second day thereafter a medium change is performed with fresh STEMdiff™ RPE Differentiation Medium D. On Day 14, immature RPE cells are enzymatically harvested and subcultured in STEMdiff™-XF RPE Maturation Medium with the addition of STEMdiff™-ACF RPE Plating Supplement from Days 14 to 21 to improve survival after passaging. RPE cells begin to mature over the course of 5 weeks of culture in STEMdiff™-XF RPE Maturation Medium and become fully matured by Day 49, possessing key characteristics such as polygonal shape, polarization, pigmentation and phagocytosis.
Figure 2. STEMdiff™ Retinal Pigment Epithelial (RPE) Plating Supplement Improves Attachment and Morphology of Replated hPSC-Derived Immature RPE Cells Derived From 4 hPSC Lines
(A) Four different cell lines of hPSC-derived immature RPE cells were replated in either STEMdiff™-XF RPE Maturation Medium alone, or (B) with added STEMdiff™-ACF RPE Plating Supplement (RPE Plating Medium). (A) Representative images of replated hPSC-derived immature RPE cells are shown 1 Day after replating. (B) hPSC-derived immature RPE cells exhibit improved attachment when replated with RPE Plating Medium. Data shown as mean + SEM; n = 3. (C) Plating Efficiency was measured across the four cell lines following replating showing a 3-4 fold increase with the STEMdiff™ RPE-ACF Plating Supplement.
Figure 3. hPSC-Derived Immature Retinal Pigment Epithelial (RPE) Cells Replated Using STEMdiff™-ACF RPE Plating Supplement Show Increased Cell Survival and Improved Morphology Post Cryopreservation
(A) Four different cell lines of cryopreserved hPSC-derived immature RPE cells were thawed and replated in either STEMdiff™-XF RPE Maturation Medium alone, or (B) with STEMdiff™-ACF RPE Plating Supplement (RPE Plating Medium). Representative images show cells 1 Day post-thaw. Post-thaw, hPSC-derived immature RPE cells exhibited improved attachment when replated with RPE Plating Medium. Data shown as mean + SEM; n = 3. (C) Post-Thaw Plating Efficiency was measured across the four cell lines following replating showing a 3-4 fold increase with the STEMdiff™-ACF RPE Plating Supplement.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
STEMdiff™ RPE Plating Supplement Catalog #
100-1364 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
STEMdiff™ RPE Plating Supplement Catalog #
100-1364 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Human Pluripotent Stem Cell Research
Workflow Stages for hPSC-Derived Sensory Research
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