产品号 #100-1364_C
无动物成分的补充剂，用于提高人多能干细胞（hPSC）衍生视网膜色素上皮细胞的存活率和贴壁能力
总览
通过在STEMdiff™-XF RPE 成熟培养基 中添加 STEMdiff™-ACF RPE 铺板补充剂，可显著提高解离、复苏和重新铺板后人多能干细胞 (hPSC) 衍生的视网膜色素上皮 (RPE) 细胞的存活率。
此补充剂旨在提高使用 STEMdiff™-ACF RPE分化试剂盒生成的 hPSC 衍生 RPE细胞的实验通量。
请将本补充剂与以下兼容的 STEMdiff™ RPE 产品配合使用，以构建完整且优化的工作流程：
STEMdiff™-ACF RPE 分化试剂盒
STEMdiff™-XF RPE 成熟培养基
如您计划将此产品用于商业或临床用途，请与我们联系。
细胞类型
神经细胞，PSC衍生，多能干细胞
应用
细胞培养
品牌
STEMdiff
研究领域
疾病建模，药物发现和毒理检测，神经科学，细胞治疗开发
Data Figures
Figure 1. Workflow for the Differentiation of hPSCs into Retinal Pigment Epithelium (RPE) with STEMdiff™ RPE Culture System
hPSC colonies, previously harvested and seeded as clumps, are put directly into the medium provided in STEMdiff™-ACF RPE Differentiation Kit. Seeding into STEMdiff™ RPE Differentiation Medium A induces cells toward immature RPE. A full medium change is performed on Day 1 with fresh STEMdiff™ RPE Differentiation Medium A and then on Day 2 with STEMdiff™ RPE Differentiation Medium B. On Days 4 and 6, medium changes are performed with fresh STEMdiff™ RPE Differentiation Medium C. On Days 7 and every second day thereafter a medium change is performed with fresh STEMdiff™ RPE Differentiation Medium D. On Day 14, immature RPE cells are enzymatically harvested and subcultured in STEMdiff™-XF RPE Maturation Medium with the addition of STEMdiff™-ACF RPE Plating Supplement from Days 14 to 21 to improve survival after passaging. RPE cells begin to mature over the course of 5 weeks of culture in STEMdiff™-XF RPE Maturation Medium and become fully matured by Day 49, possessing key characteristics such as polygonal shape, polarization, pigmentation and phagocytosis.
Figure 2. STEMdiff™ Retinal Pigment Epithelial (RPE) Plating Supplement Improves Attachment and Morphology of Replated hPSC-Derived Immature RPE Cells Derived From 4 hPSC Lines
(A) Four different cell lines of hPSC-derived immature RPE cells were replated in either STEMdiff™-XF RPE Maturation Medium alone, or (B) with added STEMdiff™-ACF RPE Plating Supplement (RPE Plating Medium). (A) Representative images of replated hPSC-derived immature RPE cells are shown 1 Day after replating. (B) hPSC-derived immature RPE cells exhibit improved attachment when replated with RPE Plating Medium. Data shown as mean + SEM; n = 3. (C) Plating Efficiency was measured across the four cell lines following replating showing a 3-4 fold increase with the STEMdiff™ RPE-ACF Plating Supplement.
Figure 3. hPSC-Derived Immature Retinal Pigment Epithelial (RPE) Cells Replated Using STEMdiff™-ACF RPE Plating Supplement Show Increased Cell Survival and Improved Morphology Post Cryopreservation
(A) Four different cell lines of cryopreserved hPSC-derived immature RPE cells were thawed and replated in either STEMdiff™-XF RPE Maturation Medium alone, or (B) with STEMdiff™-ACF RPE Plating Supplement (RPE Plating Medium). Representative images show cells 1 Day post-thaw. Post-thaw, hPSC-derived immature RPE cells exhibited improved attachment when replated with RPE Plating Medium. Data shown as mean + SEM; n = 3. (C) Post-Thaw Plating Efficiency was measured across the four cell lines following replating showing a 3-4 fold increase with the STEMdiff™-ACF RPE Plating Supplement.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
STEMdiff™ RPE Plating Supplement Catalog #
100-1364 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
STEMdiff™ RPE Plating Supplement Catalog #
100-1364 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Human Pluripotent Stem Cell Research
Workflow Stages for hPSC-Derived Sensory Research
Request Pricing
Thank you for your interest in IntestiCult™ Organoid Growth Medium (Human). Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
By submitting this form, you are providing your consent to STEMCELL Technologies Canada Inc. and its subsidiaries and affiliates (“STEMCELL”) to collect and use your information, and send you newsletters and emails in accordance with our
privacy policy. Please contact us with any questions that you may have. You can unsubscribe or change your email preferences at any time.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
欢迎您关注STEMCELL Technologies的微信公众平台
扫描二维码或搜索微信号STEMCELLTech，即可关注我们的微信平台，第一时间接收丰富的技术资源和最新的活动信息。
如您有任何问题，欢迎发消息给STEMCELLTech微信公众平台，或与我们通过电话/邮件联系：400 885 9050 INFO.CN@STEMCELL.COM。