Significantly increase the survival of human pluripotent stem cell (hPSC)-derived retinal pigment epithelial (RPE) cells following dissociation, thawing, and replating by adding the STEMdiff™-ACF RPE Plating Supplement to your STEMdiff™-XF RPE Maturation Medium.
This supplement is designed to increase your experimental throughput for hPSC-derived RPE cells generated with STEMdiff™-ACF RPE Differentiation Kit.
Use this supplement as part of a complete, optimized workflow with the following compatible STEMdiff™ RPE products:
Please contact us if you intend to use this product for commercial or clinical applications.
Cell Type Neural Cells, PSC-Derived, Pluripotent Stem Cells
Application Cell Culture
Brand STEMdiff
Area of Interest Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience, Cell Therapy Development
Data Figures
Figure 1. Workflow for the Differentiation of hPSCs into Retinal Pigment Epithelium (RPE) with STEMdiff™ RPE Culture System
hPSC colonies, previously harvested and seeded as clumps, are put directly into the medium provided in STEMdiff™-ACF RPE Differentiation Kit. Seeding into STEMdiff™ RPE Differentiation Medium A induces cells toward immature RPE. A full medium change is performed on Day 1 with fresh STEMdiff™ RPE Differentiation Medium A and then on Day 2 with STEMdiff™ RPE Differentiation Medium B. On Days 4 and 6, medium changes are performed with fresh STEMdiff™ RPE Differentiation Medium C. On Days 7 and every second day thereafter a medium change is performed with fresh STEMdiff™ RPE Differentiation Medium D. On Day 14, immature RPE cells are enzymatically harvested and subcultured in STEMdiff™-XF RPE Maturation Medium with the addition of STEMdiff™-ACF RPE Plating Supplement from Days 14 to 21 to improve survival after passaging. RPE cells begin to mature over the course of 5 weeks of culture in STEMdiff™-XF RPE Maturation Medium and become fully matured by Day 49, possessing key characteristics such as polygonal shape, polarization, pigmentation and phagocytosis.
Figure 2. STEMdiff™ Retinal Pigment Epithelial (RPE) Plating Supplement Improves Attachment and Morphology of Replated hPSC-Derived Immature RPE Cells Derived From 4 hPSC Lines
(A) Four different cell lines of hPSC-derived immature RPE cells were replated in either STEMdiff™-XF RPE Maturation Medium alone, or (B) with added STEMdiff™-ACF RPE Plating Supplement (RPE Plating Medium). (A) Representative images of replated hPSC-derived immature RPE cells are shown 1 Day after replating. (B) hPSC-derived immature RPE cells exhibit improved attachment when replated with RPE Plating Medium. Data shown as mean + SEM; n = 3. (C) Plating Efficiency was measured across the four cell lines following replating showing a 3-4 fold increase with the STEMdiff™ RPE-ACF Plating Supplement.
Figure 3. hPSC-Derived Immature Retinal Pigment Epithelial (RPE) Cells Replated Using STEMdiff™-ACF RPE Plating Supplement Show Increased Cell Survival and Improved Morphology Post Cryopreservation
(A) Four different cell lines of cryopreserved hPSC-derived immature RPE cells were thawed and replated in either STEMdiff™-XF RPE Maturation Medium alone, or (B) with STEMdiff™-ACF RPE Plating Supplement (RPE Plating Medium). Representative images show cells 1 Day post-thaw. Post-thaw, hPSC-derived immature RPE cells exhibited improved attachment when replated with RPE Plating Medium. Data shown as mean + SEM; n = 3. (C) Post-Thaw Plating Efficiency was measured across the four cell lines following replating showing a 3-4 fold increase with the STEMdiff™-ACF RPE Plating Supplement.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
