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健康对照人iPSC细胞系，男性，SCTi006-A

冻存的人多能干细胞系

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产品号 #200-0945_C

冻存的人多能干细胞系

产品优势

通过机构审查委员会 (IRB) 的方案进行收集，确保符合伦理规范，已获得学术和商业使用授权。
严格遵循国际标准（ISSCR 2023指南与ISCBI 2009规范）的全流程质控体系。
通过hPSCreg®认证的细胞系，确保研究透明度与生物伦理合规性
无缝对接标准化培养体系：TeSR™培养基（维持培养）与STEMdiff™分化试剂

产品组分包括

健康对照人iPSC细胞系，男性，SCTi006-A（产品号 #200-0945）

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To see all required products for your protocol, please consult the Protocols and Documentation.

ReLeSR™
cGMP, enzyme-free human pluripotent stem cell selection and passaging reagent
mTeSR™ Plus
cGMP, stabilized feeder-free maintenance medium for human ES and iPS cells
Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells

总览

实验数据

产品说明书及文档

应用领域

Data Figures

Table 1. iPSC Line SCTi006-A Is Derived from a Healthy Male Donor

Demographic, health, and genetic characteristics of the SCTi006-A donor are compiled based on self-reported information and whole-exome sequencing. Sex was determined by karyotype. Ancestry and HLA haplotype were calculated from whole-genome and whole-exome sequencing combined data. Blood type (ABO/Rh blood group) was determined by next-generation sequencing. Height, weight, and BMI were calculated at the donation facility. iPSC = induced pluripotent stem cell.

Figure 1. SCTi006-A Human Pluripotent Stem Cells Demonstrate High-Quality Morphology in Routine Culture

Cryopreserved cells from line SCTi006-A were thawed and maintained in mTeSR™ Plus on Corning® Matrigel® Matrix.

(A) The resulting iPSC colonies have densely packed cells and show multi-layering when ready to be passaged.

(B,C) Cells retain prominent nucleoli and high nuclear-to-cytoplasmic ratios. iPSC = induced pluripotent stem cell.

Figure 2. SCTi006-A Human Pluripotent Stem Cells Maintain a Normal Karyotype

(A) G-T-L banding for thawed cells at p14 (n = 20) shows a normal karyotype with no evidence of clonal abnormalities at a band resolution of 375 - 475 G-bands per haploid genome.

(B) Fluorescent in situ hybridization in a representative p14 iPSC using probes for 20p11 (green) and 20q11.21 (red). 95% of cells examined displayed two sets of two probe signals, indicating no aneusomy of chromosome 20 (n = 200). iPSC = induced pluripotent stem cell.

Table 2. Single Nucleotide Polymorphism Microarray Analysis Characterizes SCTi006-A Copy Number Variants

DNA was extracted from a vial of SCTi006-A from the Master Cell Bank and subject to SNP microarray analysis to identify large-scale copy number variants (CNVs). The cells display no reportable CNVs, defined as those greater than 400kb in size. Array design, genomics position, genes, and chromosome banding are based on genome build GRCh38/hg3. chr = chromosome; bp = base pairs; SNP = single nucleotide polymorphism.

Figure 3. SCTi006-A iPSCs Express Undifferentiated Cell Markers

Cell line SCTi006-A was characterized using flow cytometry for undifferentiated cell markers OCT3/4 and TRA-1-60.

(A) Percentage marker expression was quantified 3 passages after thawing from the analyses of two biological replicates.

Representative flow cytometry plots are displayed for (B) OCT3/4 and (C) TRA-1-60. iPSC = induced pluripotent stem cell.

Figure 4. SCTi006-A Human Pluripotent Stem Cells Demonstrate a High Trilineage Differentiation Capacity

Cells from SCTi006-A were split into 3 groups, differentiated using STEMdiff™ Trilineage Differentiation Kit (Catalog #05230), and then subjected to flow cytometry analysis. Two markers for each embryonic germ layer were assessed, and bars present mean marker expression for each group of cells (n = 2 biological replicates). PAX6 and Nestin confirm differentiation to the ectoderm lineage, NCAM and Brachyury (T) to the mesoderm lineage, and CXCR4 and SOX17 to the endoderm lineage.

Table 3. Comprehensive Risk Variant Profile for the SCTi006-A iPSC Line

The table presents risk variants identified for the SCTi006-A iPSC line. DNA was purified from the master cell bank, and whole-genome sequencing was performed to capture the entire genomic landscape. Sequencing was conducted on the NovaSeq 6000 System, achieving a 50x coverage. Coverage of coding regions and adjacent splice junction sites was enhanced for 20,000 genes using the SureSelect Human All Exon V6 Kit (Agilent Technologies). Variants were called and analyzed following GATK4 best practices, incorporating a Convolutional Neural Network to score each variant. High-confidence variants were annotated with predicted functional consequences and ClinVar IDs. ClinVar entries required two or more supporting ALT reads and an assertion criteria of one gold star or more in ClinVar to be considered. This comprehensive profile assists in understanding the genetic landscape and potential health impacts associated with the SCTi005-A iPSC line.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Information Sheet

Product Name

Healthy Control Human iPSC Line, Male, SCTi006-A

Catalog #

200-0945

Lot #

All

Language

English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area

Workflow Stages

Workflow Stages for Human Pluripotent Stem Cell Research

Reprogramming

Maintenance

Differentiation

Resources and Publications

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Species	Human
Contains	CryoStor® CS10
Cell And Tissue Source	Peripheral Blood, Pluripotent Stem Cells
Donor Status	Normal

Legal Statement: These iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) shall not be used or administered in (1) human subjects for human clinical use; (2) animals for veterinary use for therapeutic, diagnostic, or prophylactic purposes or (3) any subject in relation to clinical applications, cell therapy, transplantation, and/or regenerative medicines, without limiting the generality of the foregoing. These iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) may not be used for monetization or commercialization purposes, including without limitation, used to, or with the goal to, perform services or supply products or rights, including in the manufacture of cellular therapies or other therapeutics, for monetary gain or the generation of royalties, revenues, sales or other valuable consideration. For clarity, these iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) may not be used for screening compounds, antibodies, proteins or peptides, except for the purposes of target discovery, target validation, or assay development, provided such activities and the results of such activities are not further used for monetization or commercialization purposes. It may be possible to obtain a further license for the prohibited uses referred to in this Limited Use License. Please contact iPSCrequests@stemcell.com for more details. LIMITED USE LICENSE These iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) shall not be used or administered in (1) human subjects for human clinical use; (2) animals for veterinary use for therapeutic, diagnostic, or prophylactic purposes or (3) any subject in relation to clinical applications, cell therapy, transplantation, and/or regenerative medicines, without limiting the generality of the foregoing. These iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) may not be used for monetization or commercialization purposes, including without limitation, used to, or with the goal to, perform services or supply products or rights, including in the manufacture of cellular therapies or other therapeutics, for monetary gain or the generation of royalties, revenues, sales or other valuable consideration. For clarity, these iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) may not be used for screening compounds, antibodies, proteins or peptides, except for the purposes of target discovery, target validation, or assay development, provided such activities and the results of such activities are not further used for monetization or commercialization purposes. It may be possible to obtain a further license for the prohibited uses referred to in this Limited Use License. Please contact iPSCrequests@stemcell.com for more details. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.

健康对照人iPSC细胞系，男性，SCTi006-A

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健康对照人iPSC细胞系，男性，SCTi006-A

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产品号 #200-0945_C

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