产品号 #75010_C
用于哺乳动物细胞活/死染色的胺标记荧光染料
GloCell™可固定活性染料 Violet 510是一种荧光染料,适用于在流式细胞术等应用中对哺乳动物活细胞与死细胞进行染色。该染料可不可逆地结合细胞内及细胞表面的胺基,可在细胞固定、通透处理或冷冻保存之前使用。细胞膜完整性受损的细胞会允许 GloCell™ 染料进入,从而在细胞活性检测中表现出高于活细胞的荧光信号。
GloCell™可固定活性染料 Violet 510可被 405 nm 的紫色激光激发,最大荧光发射波长为 510 nm,可使用用于检测 AmCyan 的 525/50带通滤光片进行检测。
种属
人,小鼠,非人灵长类,其它细胞系,大鼠
应用
流式细胞术,免疫荧光
品牌
GloCell
研究领域
血管生成细胞研究,抗体制备,癌症,细胞系制备,嵌合体,疾病建模,药物发现和毒理检测,内皮细胞研究,上皮细胞研究,HIV,HLA,杂交瘤制备,免疫,感染性疾病(传染病),代谢,有丝分裂,神经科学,类器官,呼吸系统研究,干细胞生物学,移植研究,细胞治疗开发
Figure 1.
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with GloCell™ Fixable Viability Dye Violet 510. (B) Flow cytometry analysis of human multiple myeloma bone marrow mononuclear cells (BMMCs) labeled with GloCell™ Fixable Viability Dye Violet 510.
Figure 2.
Excitation/Emission spectra for GloCell™ Fixable Viability Dye Violet 510. Excitation max: 414 nm, Emission Max: 508 nm
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Species | Human, Mouse, Non-Human Primate, Other, Rat |
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