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抗人OCT4 （OCT3）抗体，克隆3A2A20

小鼠抗人OCT4单克隆IgG2b抗体（OCT3）
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产品号 #60093_C

小鼠抗人OCT4单克隆IgG2b抗体（OCT3）

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概述

该单克隆抗体与人八聚体结合转录因子4 （OCT4）反应，也称为OCT3和OCT3/4，是一种约40 kDa的同源结构域转录因子，属于POU家族，在未分化的人胚胎干（ES）、诱导多能干（iPS）、胚胎癌（EC）和胚胎生殖（EG）细胞中表达。OCT4结合到八聚体基序5‘-ATTTGCAT-3’上，通过与其他转录因子如SOX2相互作用，调节FBX15、FGF-4、REX1、SOX2和骨桥蛋白等基因的表达，在维持细胞多能状态中发挥关键作用。OCT4水平在分化过程中下调，因此它已成为干细胞多能性的有用标记，以及某些人类恶性生殖细胞肿瘤的标记。OCT4与其他转录因子的表达已被用于将体细胞重编程为iPS细胞。已经观察到多种OCT4亚型，在人类中至少有两种具有功能活性。

Subtype
Primary Antibodies
 
Target Antigen
OCT4 (OCT3)
 
Alternative Names
OCT-3, OCT-4, OCT3, octamer-binding transcription factor 4, POU domain class 5 transcription factor 1, POU5F1
 
Reactive Species
Human
 
Conjugation
Alexa Fluor 488, PE, Unconjugated
 
Host Species
Mouse
 
Cell Type
Pluripotent Stem Cells
 
Species
Human
 
Application
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Western Blotting
 
Area of Interest
Stem Cell Biology
 
Clone
3A2A20
 
Gene ID
5460
 
Isotype
IgG2b, kappa
 

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Data Figures

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of ES cells cultured with mTeSR™1 on Corning® Matrigel®. The ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control; dashed line histogram) were fixed and labeled with Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, Alexa Fluor® 488. Labeling of the ES cells with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Alexa Fluor® 488 (Catalog #60072AD) is shown (solid line histogram). (B) Human ES cells were cultured with TeSR™-E8™ on glass coverslips coated with Vitronectin XF™ (Catalog #07180), then fixed and labeled with Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, Alexa Fluor® 488. Inset shows labeling of human ES cells with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Alexa Fluor® 488. (C) DAPI counterstaining of the cells shown in figure (B); nuclear localization of the OCT4 (OCT3) marker is evident.

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of human ES cells cultured with mTeSR™1 on Corning® Matrigel®. The ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control; dashed line histogram) were fixed and labeled with Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, PE. Labeling of the ES cells with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PE (Catalog #60072PE) is shown (solid line histogram). (B) Human ES cells were cultured with TeSR™-E8™ on glass coverslips coated with Vitronectin XF™ (Catalog #07180), then fixed and labeled with Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, PE. Inset shows labeling of human ES cells with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PE. (C) DAPI counterstaining of the cells shown in figure (B); nuclear localization of the OCT4 (OCT3) marker is evident.

Data for Unconjugated

Figure 3. Data for Unconjugated

(A) Human induced pluripotent stem (iPS) cells were cultured with TeSR™-E8™ on glass coverslips coated with Vitronectin XF™ (Catalog #07180), then fixed and stained with Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with a mouse IgG2b, kappa isotype control antibody followed by goat anti-mouse IgG, FITC.
(B) Flow cytometry analysis of human embryonic stem (ES) cells cultured with mTeSR™1 on Corning® Matrigel®. The ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control, dashed line histogram) were fixed and labeled with Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, followed by goat anti-mouse IgG, FITC. Labeling of the ES cells with a mouse IgG2b, kappa isotype control antibody followed by goat anti-mouse IgG, FITC is shown (solid line histogram). (C) Flow cytometry analysis of human iPS cells cultured with TeSR™-E8™ on Vitronectin XF™. The cells were fixed and labeled with Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, followed by goat anti-mouse IgG, FITC (filled histogram) or a mouse IgG2b, kappa isotype control antibody followed by goat anti-mouse IgG, FITC (solid line histogram). (D) Western blot analysis of denatured/reduced cell lysates with Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20. Lane 1, human ES cells cultured with mTeSR™1 on Corning® Matrigel®, lane 2 (negative control), mouse E13.5 neural progenitor cells cultured with NeuroCult™ Proliferation Kit (Mouse, Catalog #05702).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20
Catalog #
60093.1, 60093
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, PE
Catalog #
60093PE.1, 60093PE
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, Alexa Fluor® 488
Catalog #
60093AD.1, 60093AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20
Catalog #
60093.1, 60093
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, PE
Catalog #
60093PE.1, 60093PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, Alexa Fluor® 488
Catalog #
60093AD.1, 60093AD
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (3)

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hPSC Differentiation: Tools for Pluripotent Stem Cell-Derived Research
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Products for Human Pluripotent Stem Cells
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Products for Human Pluripotent Stem Cells

Publications (2)

Trend of telomerase activity change during human iPSC self-renewal and differentiation revealed by a quartz crystal microbalance based assay. Zhou Y et al. Scientific reports 2014 NOV

Abstract

Telomerase plays an important role in governing the life span of cells for its capacity to extend telomeres. As high activity of telomerase has been found in stem cells and cancer cells specifically, various methods have been developed for the evaluation of telomerase activity. To overcome the time-consuming procedures and complicated manipulations of existing methods, we developed a novel method named Telomeric Repeat Elongation Assay based on Quartz crystal microbalance (TREAQ) to monitor telomerase activity during the self-renewal and differentiation of human induced pluripotent stem cells (hiPSCs). TREAQ results indicated hiPSCs possess invariable telomerase activity for 11 passages on Matrigel and a steady decline of telomerase activity when differentiated for different periods, which is confirmed with existing golden standard method. The pluripotency of hiPSCs during differentiation could be estimated through monitoring telomerase activity and compared with the expression levels of markers of pluripotency gene via quantitative real time PCR. Regular assessment for factors associated with pluripotency or stemness was expensive and requires excessive sample consuming, thus TREAQ could be a promising alternative technology for routine monitoring of telomerase activity and estimate the pluripotency of stem cells.
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Evidences for the involvement of cell surface glycans in stem cell pluripotency and differentiation Alisson-Silva F et al. Glycobiology 2014 MAY

Abstract

Induced pluripotent stem (iPS) cells are somatic cells that have been reprogrammed to a pluripotent state via the introduction of defined transcription factors. Although iPS is a potentially valuable resource for regenerative medicine and drug development, several issues regarding their pluripotency, differentiation propensity and potential for tumorigenesis remain to be elucidated. Analysis of cell surface glycans has arisen as an interesting tool for the characterization of iPS. An appropriate characterization of glycan surface molecules of human embryonic stem (hES) cells and iPS cells might generate crucial data to highlight their role in the acquisition and maintenance of pluripotency. In this study, we characterized the surface glycans of iPS generated from menstrual blood-derived mesenchymal cells (iPS-MBMC). We demonstrated that, upon spontaneous differentiation, iPS-MBMC present high amounts of terminal $\$-galactopyranoside residues, pointing to an important role of terminal-linked sialic acids in pluripotency maintenance. The removal of sialic acids by neuraminidase induces iPS-MBMC and hES cells differentiation, prompting an ectoderm commitment. Exposed $\$-galactopyranose residues might be recognized by carbohydrate-binding molecules found on the cell surface, which could modulate intercellular or intracellular interactions. Together, our results point for the first time to the involvement of the presence of terminal sialic acid in the maintenance of embryonic stem cell pluripotency and, therefore, the modulation of sialic acid biosynthesis emerges as a mechanism that may govern stem cell differentiation.
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更多信息

更多信息
Species Human
Clone 3A2A20
Gene Id 5460
Alternative Names OCT-3, OCT-4, OCT3, octamer-binding transcription factor 4, POU domain class 5 transcription factor 1, POU5F1
Isotype IgG2b, kappa
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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