GloCell™ Fixable Viability Dye Red 780 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be utilized prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in a greater fluorescence compared to live cells in a cell viability assay.
GloCell™ Fixable Viability Dye Red 780 is excited by the red laser at 633 nm and it has a fluorescence emission maximum of 780 nm that can be detected using the 780/60 band pass filter used to detect APC-Cy7.
GloCell™ Fixable Viability Dye Red 780 is excited by the red laser at 633 nm and it has a fluorescence emission maximum of 780 nm that can be detected using the 780/60 band pass filter used to detect APC-Cy7.
Data Figures
Figure 1.
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with GloCell™ Fixable Viability Dye Red 780. (B) Flow cytometry analysis of human multiple myeloma bone marrow mononuclear cells (BMMCs) labeled with GloCell™ Fixable Viability Dye Red 780.
Figure 2.
Excitation/Emission spectra for GloCell™ Fixable Viability Dye Red 780. Excitation max: 757 nm, Emission Max: 779 nm
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
GloCell™ Fixable Viability Dye Red 780
Catalog #
75007, 75007.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
GloCell™ Fixable Viability Dye Red 780
Catalog #
75007.1
Lot #
All
Language
English
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for B Cell Research
Workflow Stages for Brain Tumor Stem Cell Research
Workflow Stages for Airway Epithelial Cell Research
Workflow Stages for CHO and Other Cell Line Development
Workflow Stages for Dendritic Cell Research
Workflow Stages for Human Pluripotent Stem Cell Research
Workflow Stages for Granulocyte Research
Workflow Stages for Hematopoietic Stem and Progenitor Cell Research
Workflow Stages for hPSC-Derived Endoderm Cell Research
Workflow Stages for hPSC-Derived Neural Cell Research
Workflow Stages for hPSC-Derived Mesoderm Cell Research
Workflow Stages for Hybridoma Production
Workflow Stages for Intestinal Epithelial Cell Research
Workflow Stages for Total Lymphocyte Research
Workflow Stages for Mammary Epithelial Cell Research
Workflow Stages for Mesenchymal Stem and Progenitor Cell Research
Workflow Stages for Monocyte Research
Workflow Stages for Mouse Pluripotent Stem Cell Research
Workflow Stages for Myeloid Cell Research
Workflow Stages for Neural Stem and Progenitor Cell Research
Workflow Stages for Neuronal Research
Workflow Stages for Natural Killer Cell Research
Workflow Stages for Prostate Epithelial Cell Research
Workflow Stages for T Cell Research
Workflow Stages for HLA Analysis
Workflow Stages for Innate Lymphoid Cell Research
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