产品号 #75007_C
Amine-labeling fluorescent dye for live/dead staining of mammalian cells
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
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EasySep™ BufferCell separation buffer
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D-PBS (Without Ca++ and Mg++)Dulbecco’s phosphate-buffered saline without calcium and magnesium
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Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells
概述
GloCell™ Fixable Viability Dye Red 780 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be utilized prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in a greater fluorescence compared to live cells in a cell viability assay.
GloCell™ Fixable Viability Dye Red 780 is excited by the red laser at 633 nm and it has a fluorescence emission maximum of 780 nm that can be detected using the 780/60 band pass filter used to detect APC-Cy7.
Species
Human, Mouse, Non-Human Primate, Other, Rat
Application
Flow Cytometry, Immunofluorescence
Brand
GloCell
Area of Interest
Angiogenic Cell Research, Antibody Development, Cancer, Cell Line Development, Chimerism, Disease Modeling, Drug Discovery and Toxicity Testing, Endothelial Cell Biology, Epithelial Cell Biology, HIV, HLA, Hybridoma Generation, Immunology, Infectious Diseases, Metabolism, Mitosis, Neuroscience, Organoids, Respiratory Research, Stem Cell Biology, Transplantation Research, Cell Therapy Development
Data Figures
Figure 1.
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with GloCell™ Fixable Viability Dye Red 780. (B) Flow cytometry analysis of human multiple myeloma bone marrow mononuclear cells (BMMCs) labeled with GloCell™ Fixable Viability Dye Red 780.
Figure 2.
Excitation/Emission spectra for GloCell™ Fixable Viability Dye Red 780. Excitation max: 757 nm, Emission Max: 779 nm
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
GloCell™ Fixable Viability Dye Red 780 Catalog #
75007, 75007.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
GloCell™ Fixable Viability Dye Red 780 Catalog #
75007.1 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for B Cell Research
Workflow Stages for Brain Tumor Stem Cell Research
Workflow Stages for Airway Epithelial Cell Research
Workflow Stages for CHO and Other Cell Line Development
Workflow Stages for Dendritic Cell Research
Workflow Stages for Human Pluripotent Stem Cell Research
Workflow Stages for Granulocyte Research
Workflow Stages for Hematopoietic Stem and Progenitor Cell Research
Workflow Stages for hPSC-Derived Endoderm Cell Research
Workflow Stages for hPSC-Derived Neural Cell Research
Workflow Stages for hPSC-Derived Mesoderm Cell Research
Workflow Stages for Hybridoma Production
Workflow Stages for Intestinal Epithelial Cell Research
Workflow Stages for Total Lymphocyte Research
Workflow Stages for Mammary Epithelial Cell Research
Workflow Stages for Mesenchymal Stem and Progenitor Cell Research
Workflow Stages for Monocyte Research
Workflow Stages for Mouse Pluripotent Stem Cell Research
Workflow Stages for Myeloid Cell Research
Workflow Stages for Neural Stem and Progenitor Cell Research
Workflow Stages for Neuronal Research
Workflow Stages for Natural Killer Cell Research
Workflow Stages for Prostate Epithelial Cell Research
Workflow Stages for T Cell Research
Workflow Stages for HLA Analysis
Workflow Stages for Innate Lymphoid Cell Research
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