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EasySep™小鼠TIL（CD45）正选试剂盒

小鼠CD45+ TIL免疫磁珠正选
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产品号 #（选择产品）

产品号 #100-0350_C

小鼠CD45+ TIL免疫磁珠正选

产品优势

  • 操作简单、快捷，且无需分离柱
  • 针对肿瘤和CD45起始占比低的组织样本进行优化
  • 实验方法可灵活调整以实现更高的纯度或回收率

产品组分包括

  • EasySep™ Mouse TIL (CD45) Positive Selection Cocktail Component A, 0.25 mL
  • EasySep™ Mouse TIL (CD45) Positive Selection Cocktail Component B, 0.25 mL
  • EasySep™ Mouse TIL (CD45) Positive Selection Cocktail Component C, 1.5 mL
  • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
main product photo
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

使用EasySep™小鼠TIL（CD45）正选试剂盒，通过免疫磁珠正选轻松分离高纯度的小鼠CD45+肿瘤浸润白细胞（TIL）。该试剂盒已针对小鼠实体瘤的单细胞悬液进行了优化，包括将4T1、B16-F10和CT26.WT细胞系植入同品系小鼠来诱导的肿瘤。由于不同小鼠肿瘤的异质性，该试剂盒可能需要优化。EasySep™技术结合单克隆抗体的特异性和无柱磁分选系统的简便性，已在发表的研究中广泛应用超过20年。

在该EasySep™正选流程中，目标细胞通过识别CD45的抗体复合物和磁珠进行标记，并通过EasySep™磁极分选。只需简单倾倒或吸取以弃去非目的细胞，而目的细胞则保留在试管中。磁珠分选后，目的CD45+ TIL可立即用于流式细胞术、培养和基于细胞的实验等下游应用。

了解更多关于EasySep™免疫磁珠分选技术的工作原理。探索更多为您实验流程优化的其它产品，包括培养基、补充剂、抗体等。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103)
 
亚型
细胞分选试剂盒
 
细胞类型
白细胞
 
种属
小鼠
 
样本来源
其它细胞系，Tumor
 
筛选方法
Positive
 
应用
细胞分选
 
品牌
EasySep
 
研究领域
癌症，免疫
 

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Data Figures

Using the EasySep™ Mouse TIL (CD45) Positive Selection Kit, the CD45+ TIL purities of the start and final isolated fractions are 14.8% and 95.2%, respectively for B16-F10 tumor single-cell suspension, 37.6% and 93.0%, respectively for 4T1 tumor single-cell suspension, and 32.3% and 84.6%, respectively for CT26.WT tumor single-cell suspension. Using the EasySep™ Mouse TIL (CD45) Positive Selection Kit, the CD45+ TIL purities of the start and final isolated fractions are 14.8% and 95.2%, respectively for B16-F10 tumor single-cell suspension, 37.6% and 93.0%, respectively for 4T1 tumor single-cell suspension, and 32.3% and 84.6%, respectively for CT26.WT tumor single-cell suspension. Using the EasySep™ Mouse TIL (CD45) Positive Selection Kit, the CD45+ TIL purities of the start and final isolated fractions are 14.8% and 95.2%, respectively for B16-F10 tumor single-cell suspension, 37.6% and 93.0%, respectively for 4T1 tumor single-cell suspension, and 32.3% and 84.6%, respectively for CT26.WT tumor single-cell suspension.

Figure 1. Typical EasySep™ Mouse TIL (CD45) Cell Isolation Profile

Tumors were induced by B16-F10, 4T1, or CT26.WT cell lines and dissociated into single-cell suspensions. CD45+ TILs were isolated from single-cell suspensions at various start concentrations using the purple EasySep™ Magnet.

(A) Starting with a B16-F10 tumor single-cell suspension at 1 x 10⁸ cells/mL, the purities of the start and final isolated fractions are 14.8% and 95.2%, respectively.

(B) Starting with a 4T1 tumor single-cell suspension at 4 x 10⁷ cells/mL, the purities of the start and final isolated fractions are 37.6% and 93.0%, respectively.

(C) Starting with a CT26.WT tumor single-cell suspension at 2.5 x 10⁷ cells/mL, the purities of the start and final isolated fractions are 32.3% and 84.6%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ Mouse TIL (CD45) Positive Selection Kit
Catalog #
100-0350
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Mouse TIL (CD45) Positive Selection Kit
Catalog #
100-0350
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Mouse TIL (CD45) Positive Selection Kit
Catalog #
100-0350
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Mouse TIL (CD45) Positive Selection Kit
Catalog #
100-0350
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Mouse TIL (CD45) Positive Selection Kit
Catalog #
100-0350
Lot #
All
Language
English

更多信息

更多信息
Species Mouse
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103)
Sample Source Other, Tumor
Selection Method Positive
标记抗体
  1. 抗小鼠CD45抗体，克隆30-F11
    抗小鼠CD45抗体，克隆30-F11

    大鼠单克隆IgG2b抗体，抗小鼠CD45

  2. 抗小鼠CD45.2抗体，克隆104
    抗小鼠CD45.2抗体，克隆104

    抗小鼠CD45.2小鼠单克隆抗体

  3. 抗小鼠CD45.1抗体，克隆A20
    抗小鼠CD45.1抗体，克隆A20

    抗小鼠CD45.1小鼠单克隆抗体

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