产品号 #07912_C
配置于DMEM中的10 X 胶原酶/透明质酸酶
总览
使用配置于Dulbecco 's Modified Eagle 's Medium （DMEM）中的10X胶原酶/透明质酸酶酶解人乳腺细胞。该蛋白酶/多糖酶组合可以消化天然胶原纤维，并水解结缔组织中的透明质酸，从而有效地解离组织。胶原酶/透明质酸酶也可用于前列腺上皮细胞的酶解。
包含
• 3000 U/mL Collagenase
• 1000 U/mL Hyaluronidase
• DMEM (1000 mg D-glucose/L)
亚型
酶法相关（或酶解类产品
细胞类型
乳腺细胞，其它细胞系，前列腺细胞
种属
人，小鼠，非人灵长类，其它细胞系，大鼠
应用
细胞培养
研究领域
上皮细胞研究
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (2)
Publications (1)
Silica bioreplication preserves three-dimensional spheroid structures of human pluripotent stem cells and HepG2 cells. Scientific reports 2015 SEP
Abstract
Three-dimensional (3D) cell cultures produce more in vivo-like multicellular structures such as spheroids that cannot be obtained in two-dimensional (2D) cell cultures. Thus, they are increasingly employed as models for cancer and drug research, as well as tissue engineering. It has proven challenging to stabilize spheroid architectures for detailed morphological examination. Here we overcome this issue using a silica bioreplication (SBR) process employed on spheroids formed from human pluripotent stem cells (hPSCs) and hepatocellular carcinoma HepG2 cells cultured in the nanofibrillar cellulose (NFC) hydrogel. The cells in the spheroids are more round and tightly interacting with each other than those in 2D cultures, and they develop microvilli-like structures on the cell membranes as seen in 2D cultures. Furthermore, SBR preserves extracellular matrix-like materials and cellular proteins. These findings provide the first evidence of intact hPSC spheroid architectures and similar fine structures to 2D-cultured cells, providing a pathway to enable our understanding of morphogenesis in 3D cultures.
更多信息
| Species | Human, Mouse, Non-Human Primate, Other, Rat |
|---|---|
| Contains | • 3000 U/mL Collagenase • 1000 U/mL Hyaluronidase • DMEM (1000 mg D-glucose/L) |
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
