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抗小鼠CD45抗体，克隆30-F11

大鼠单克隆IgG2b抗体，抗小鼠CD45
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产品号 #（选择产品）

产品号 #60030_C

大鼠单克隆IgG2b抗体，抗小鼠CD45

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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

30-F11抗体识别小鼠CD45的所有亚型和同种异构抗原（CD45.1和CD45.2）上的一个细胞外表位。CD45是一种I型跨膜糖蛋白，在大多数血细胞表面表达，除了成熟红细胞、血小板和浆细胞外（这些细胞类型在分化过程中会丢失CD45表达）。CD45是蛋白酪氨酸磷酸酶家族的一员，在许多免疫调节过程中起作用，包括细胞活化、生长、分化和致癌转化。CD45的大细胞质部分包含两个酪氨酸磷酸酶结构域（其中一个具有酶活性），参与调节细胞内底物（如Src激酶Lck和Fyn）的功能。CD45的几种亚型已经被鉴定出来，其表达根据细胞类型和功能状态而不同。编码胞外RA， RB和RC多肽序列的三个外显子（4,5,6）的选择性剪接产生了多达8个分子量在180 - 240 kDa范围内的异构体。

亚型
一抗
 
靶抗原
CD45
 
别名
LCA，白细胞共同抗原，Ly-5，C型蛋白酪氨酸磷酸酶受体，PTPRC，T200
 
活性物种
小鼠
 
偶联
Alexa Fluor 488，APC，Biotin 或 生物素，FITC，PE，PerCP-Cyanine5.5，未偶联的
 
宿主物种
大鼠
 
细胞类型
粒细胞及其亚群，造血干/祖细胞，淋巴细胞，髓系细胞
 
种属
小鼠
 
应用
CyTOF，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹
 
研究领域
免疫
 
克隆
30-F11
 
基因编号
19264
 
同种型
IgG2b，kappa
 

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Data Figures

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, followed by a mouse anti-rat IgG2b antibody, FITC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC (solid line histogram).

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, Alexa Fluor® 488 (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Alexa Fluor® 488 (Catalog #60077AD) (solid line histogram).

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, APC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) (solid line histogram).

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Biotin (Catalog #60077BT), followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) (solid line histogram).

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, PE (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, PE (Catalog #60077PE) (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, PerCP-Cy5.5 (filled histogram) or a rat IgG2b, kappa PerCP-Cy5.5 isotype control antibody (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, Alexa Fluor® 488
Catalog #
60030AD, 60030AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, APC
Catalog #
60030AZ, 60030AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, Biotin
Catalog #
60030BT.1, 60030BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, FITC
Catalog #
60030FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, PE
Catalog #
60030PE, 60030PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, PerCP-Cy5.5
Catalog #
60030PS, 60030PS.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11
Catalog #
60030.1, 60030
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, Alexa Fluor® 488
Catalog #
60030AD, 60030AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, APC
Catalog #
60030AZ, 60030AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, Biotin
Catalog #
60030BT.1, 60030BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, FITC
Catalog #
60030FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, PE
Catalog #
60030PE, 60030PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, PerCP-Cy5.5
Catalog #
60030PS, 60030PS.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11
Catalog #
60030.1, 60030
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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更多信息
Species Mouse
Clone 30-F11
Gene Id 19264
Alternative Names LCA, Leukocyte common antigen, Ly-5, Protein tyrosine phosphatase receptor type C, PTPRC, T200
Isotype IgG2b, kappa
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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