产品号 #60117_C
小鼠抗小鼠CD45.1单克隆抗体
该单克隆抗体(克隆A20)与小鼠分化异体抗原45.1 (CD45.1)(也称为Ly5.1)和白细胞共同抗原(180 - 240 kDa的I型跨膜糖蛋白)反应。CD45.1是CD45的一种等位基因形式,在小鼠DA、RIII、SJL/J和STS/A株中,除成熟红细胞和血小板外,所有造血细胞都表达CD45。在需要检测CD45.1的应用中使用该重组单克隆抗体,如流式细胞术(FC)。该抗体克隆已通过EasySep™小鼠CD45阳性选择试剂盒(目录#18945)或EasySep™小鼠TIL (CD45)阳性选择试剂盒(目录#100-0350)分离的细胞纯度评估验证。
克隆A20不与表达CD45.2同种抗原的白细胞发生反应。CD45是蛋白酪氨酸磷酸酶(PTP)家族的一员,在细胞内(COOH)结构域含有两个催化结构域,调节细胞信号传导。由于外显子4、5和6之间的选择性剪接以及不同的糖基化,细胞外结构域是高度可变的。这些CD45亚型与特定的细胞类型以及免疫细胞的特定激活和成熟状态相关。该单克隆抗体已广泛用于小鼠过继细胞转移和骨髓移植研究,允许CD45.2+和CD45.1+细胞分化。
Subtype
Primary Antibodies
Target Antigen
CD45.1
Reactive Species
Mouse
Conjugation
APC, Biotin, FITC, PE
Host Species
Mouse
Cell Type
Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Lymphocytes, Myeloid Cells
Species
Mouse
Application
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Area of Interest
Immunology
Clone
A20
Gene ID
19264
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Thank you for your interest in IntestiCult™ Organoid Growth Medium (Human). Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
Species | Mouse |
---|---|
Clone | A20 |
Gene Id | 19264 |
小鼠CD45+ TILs的免疫磁阳性选择
小鼠CD45+细胞的免疫磁阳性选择
大鼠单克隆IgG2a, kappa同型控制抗体
小鼠抗小鼠CD45.2单克隆抗体
扫描二维码或搜索微信号STEMCELLTech,即可关注我们的微信平台,第一时间接收丰富的技术资源和最新的活动信息。
如您有任何问题,欢迎发消息给STEMCELLTech微信公众平台,或与我们通过电话/邮件联系:400 885 9050 INFO.CN@STEMCELL.COM。