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EasySep™ Mouse CD138 Positive Selection Kit

Immunomagnetic positive selection of mouse CD138+ cells
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产品优势


  • Fast, easy-to-use and column-free

  • Up to 97% purity with immunized mice

  • Useful for the enrichment of plasma cells

  • Compatible with hybridoma generation protocols, including electrofusion

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    概述
    实验数据
    产品说明书及文档
    应用领域
    相关材料与文献
    Isolate highly purified mouse CD138+ cells from mouse splenocytes, lymph nodes, or bone marrow samples by immunomagnetic positive selection, with the EasySep™ Mouse CD138 Positive Selection Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

    In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing CD138 and magnetic particles. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD138+ cells are ready for downstream applications such as flow cytometry, cell culture, and hybridoma generation.

    Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.

    Data Figures

    Typical EasySep™ Mouse CD138 Positive Cell Isolation Profile

    Figure 1. Typical EasySep™ Mouse CD138 Positive Cell Isolation Profile

    (A) Starting with immunized BALB/c mouse splenocytes, the CD138+ cell content of the isolated fraction is typically 81.5 ± 4.9% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 4.6% and 83.3%, respectively.

    (B) Starting with immunized BALB/c mouse splenocytes, the plasma cell (CD138+CD267 (TACI)+) content is typically 68.5 ± 11.3% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 1.3% and 70.2%, respectively.

    (C) Starting with naïve C57BL/6 mouse splenocytes, the CD138+ cell content of the isolated fraction is typically 78.3 ± 5.7% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 5.0% and 79.5%, respectively.

    (D) Starting with naïve C57BL/6 mouse splenocytes, the plasma cell (CD138+CD267 (TACI)+) content is typically 50.8 ± 10.0 % (mean ± SD). In the above example, the purities of the start and final isolated fractions are 0.4% and 56.3%, respectively.

    (E) Isolated CD138+ cells or total splenocytes from mice immunized with various antigens were fused with Sp2/0 mouse myeloma cells and plated in semi-solid medium using ClonaCell™-HY Hybridoma Kit (Catalog #03800). The % antigen-specific hit rate was determined by ELISA. The % antigen-specific hit rates for total splenocytes and CD138+ cells were 4.1 ± 1.6% and 28.8 ± 11.0% (mean ± SD), respectively.

    (F) Isolated CD138+ cells or total splenocytes from mice immunized with various antigens were fused with Sp2/0 mouse myeloma cells and plated in semi-solid medium using ClonaCell™-HY Hybridoma Kit (Catalog #03800). The % antibody-secreting hybridomas was determined by ELISA. The % antibody-secreting hybridomas for total splenocytes and CD138+ cells were 33.0 ± 8.7% and 99.3 ± 0.6% (mean ± SD), respectively.

    Protocols and Documentation

    Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

    Document Type
    Product Name
    Catalog #
    Lot #
    Language
    Document Type
    Product Information Sheet
    Product Name
    EasySep™ Mouse CD138 Positive Selection Kit
    Catalog #
    18957
    Lot #
    All
    Language
    English
    Document Type
    Product Information Sheet
    Product Name
    RoboSep™ Mouse CD138 Positive Selection Kit
    Catalog #
    18957RF
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 1
    Product Name
    EasySep™ Mouse CD138 Positive Selection Kit
    Catalog #
    18957
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 2
    Product Name
    EasySep™ Mouse CD138 Positive Selection Kit
    Catalog #
    18957
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 3
    Product Name
    EasySep™ Mouse CD138 Positive Selection Kit
    Catalog #
    18957
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 1
    Product Name
    RoboSep™ Mouse CD138 Positive Selection Kit
    Catalog #
    18957RF
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 2
    Product Name
    RoboSep™ Mouse CD138 Positive Selection Kit
    Catalog #
    18957RF
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 3
    Product Name
    RoboSep™ Mouse CD138 Positive Selection Kit
    Catalog #
    18957RF
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 4
    Product Name
    RoboSep™ Mouse CD138 Positive Selection Kit
    Catalog #
    18957RF
    Lot #
    All
    Language
    English

    Applications

    This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

    Research Area
    Workflow Stages
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