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抗小鼠CD45R抗体，克隆RA3-6B2

大鼠单克隆IgG2a抗体，抗人、小鼠、猫CD45R（B220）
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产品号 #60019_C

大鼠单克隆IgG2a抗体，抗人、小鼠、猫CD45R（B220）

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总览
实验数据
产品说明书及文档
应用领域
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总览

RA3-6B2抗体识别小鼠CD45外显子A限制性异构体胞外结构域上的一个表位。CD45是蛋白酪氨酸磷酸酶家族的一员，在许多免疫调节过程中起作用，包括细胞活化、生长、分化和致癌转化。CD45的大细胞质部分包含两个酪氨酸磷酸酶结构域（其中一个具有酶活性），参与调节Src激酶（如Lck和Fyn）的功能。CD45的几种亚型已经被鉴定出来，其表达根据细胞类型和功能状态而不同。编码胞外RA， RB和RC多肽序列的三个外显子的选择性剪接产生多达8个分子质量在200 - 220 kDa范围内的异构体。小鼠CD45的外显子A限制性异构体，又称为CD45R，包含RA， RB和RC区域，是最大的异构体。CD45R在B细胞谱系（早期pro-B细胞到成熟B细胞）上表达，在向浆细胞分化的过程中表达下调。CD45R也在T、NK、树突状细胞和淋巴因子激活的杀伤细胞（LAK）亚群上表达。

该抗体克隆已被验证可用于评估EasySep™试剂盒（包括EasySep™小鼠B细胞分选试剂盒（产品号#19854））分选的细胞纯度。

亚型
一抗
 
靶抗原
CD45R (B220)
 
别名
B220，蛋白酪氨酸磷酸酶受体C型，PTPRC
 
活性物种
Cat，人，小鼠
 
偶联
Biotin 或 生物素，FITC，PE，未偶联的
 
宿主物种
大鼠
 
细胞类型
B 细胞，白细胞，淋巴细胞
 
种属
人，小鼠，其它细胞系
 
应用
CyTOF，FACS，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹
 
研究领域
免疫，移植研究
 
克隆
RA3-6B2
 
基因编号
19264/5788
 
同种型
IgG2a，kappa
 

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Data Figures

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, PE (Catalog #60076PE) (solid line histogram).

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ) (solid line histogram).

Data for FITC-Conjugated

Figure 4. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC (Catalog #60076FI) (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 5. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis fo C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Data for Alexa Fluor® 488-Conjugated

Figure 6. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, Alexa Fluor® 488 (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Alexa Fluor® 488 (Catalog #60076AD) (solid line histogram).

Data for Biotin-Conjugated

Figure 7. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT), followed by SAV APC (solid line histogram).

Figure 8. Data for PB-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, Pacific Blue™ (filled histogram) or a rat IgG2a, kappa Pacific Blue™ isotype control antibody (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, Alexa Fluor® 488
Catalog #
60019AD, 60019AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC
Catalog #
60019AZ, 60019AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, Biotin
Catalog #
100-0425, 100-0424, 60019BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC
Catalog #
100-0423, 60019FI
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, Pacific Blue™
Catalog #
60019PB, 60019PB.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE
Catalog #
100-0421, 100-0420
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, PerCP-Cy5.5
Catalog #
60019PS, 60019PS.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2
Catalog #
100-0422, 60019
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, Alexa Fluor® 488
Catalog #
60019AD, 60019AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC
Catalog #
60019AZ, 60019AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, Biotin
Catalog #
100-0425, 100-0424, 60019BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC
Catalog #
100-0423, 60019FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, Pacific Blue™
Catalog #
60019PB, 60019PB.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE
Catalog #
100-0421, 100-0420
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, PerCP-Cy5.5
Catalog #
60019PS, 60019PS.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2
Catalog #
100-0422, 60019
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Species Human, Mouse, Other
Clone RA3-6B2
Gene Id 19264/5788
Alternative Names B220, Protein tyrosine phosphatase receptor type C, PTPRC
Isotype IgG2a, kappa
Legal Statement: Alexa Fluor and Pacific Blue are trademarks of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® or Pacific Blue™ are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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