产品号 #60035_C
Rat monoclonal IgG2a antibody against mouse CD138 (syndecan-1)
概述
The 281-2 antibody reacts with an extracellular epitope on the core protein of mouse CD138 (Syndecan-1), a ~60 - 100 kDa type 1 transmembrane glycoprotein expressed on the surface of pre-B cells, immature B cells, and normal and malignant plasma cells (but not mature circulating B cells). CD138 is also expressed on non-hematopoietic cells such as embryonic mesenchymal cells, endothelial, epithelial and neural cells. CD138 is thought to act primarily as a receptor that modulates cell proliferation, cell migration and cell-matrix associations by linking the extracellular matrix to the cytoskeleton. Heparin sulfate and chondroitin sulfate moieties attached to CD138 associate with several proteins, including collagens, fibronectin, tenascin, thrombospondin and certain cytokines.
Subtype
Primary Antibodies
Target Antigen
CD138 (Syndecan-1)
Alternative Names
B-B4, SDC1, syndecan-1
Reactive Species
Mouse
Conjugation
APC, Biotin, PE, Unconjugated
Host Species
Rat
Cell Type
B Cells, Plasma
Species
Mouse
Application
Cell Isolation, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Cancer, Immunology
Clone
281-2
Gene ID
20969
Isotype
IgG2a, kappa
Data Figures
Figure 1. Data for Unconjugated
Flow cytometry analysis of Sp2/0 mouse myeloma cells labeled with Anti-Mouse CD138 Antibody, Clone 281-2, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).
Figure 2. Data for APC-Conjugated
(A) Flow cytometry analysis of Sp2/0 mouse myeloma cells labeled with Anti-Mouse CD138 Antibody, Clone 281-2, APC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ) (solid line histogram). (B) Flow cytometry analysis of naïve C57BL/6 mouse splenocytes labeled with Anti-Mouse CD138 Antibody, Clone 281-2, APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC (Catalog #60019FI). (C) Flow cytometry analysis of naïve C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC.
Figure 3. Data for Biotin-Conjugated
(A) Flow cytometry analysis of Sp2/0 mouse myeloma cells labeled with Anti-Mouse CD138 Antibody, Clone 281-2, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT), followed by SAV APC (solid line histogram). (B) Flow cytometry analysis of naïve C57BL/6 mouse splenocytes labeled with Anti-Mouse CD138 Antibody, Clone 281-2, Biotin, followed by SAV APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC (Catalog #60019FI). (C) Flow cytometry analysis of naïve C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin, followed by SAV APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC.
Figure 4. Data for PE-Conjugated
(A) Flow cytometry analysis of Sp2/0 mouse myeloma cells labeled with Anti-Mouse CD138 Antibody, Clone 281-2, PE (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, PE (Catalog #60076PE) (solid line histogram). (B) Flow cytometry analysis of naïve C57BL/6 mouse splenocytes labeled with Anti-Mouse CD138 Antibody, Clone 281-2, PE and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC (Catalog #60019AZ). (C) Flow cytometry analysis of naïve C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, PE and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Mouse CD138 (Syndecan-1) Antibody, Clone 281-2 Catalog #
60035, 60035.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse CD138 (Syndecan-1) Antibody, Clone 281-2 Catalog #
60035, 60035.1 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for B Cell Research
Workflow Stages for Total Lymphocyte Research
Resources and Publications
Educational Materials (3)
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