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EasySep™人Th17细胞富集试剂盒 II

采用可解离磁珠对人Th17（CD4+CXCR3-CCR6+）细胞进行免疫磁珠分选
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产品号 #（选择产品）

产品号 #17862_C

采用可解离磁珠对人Th17（CD4+CXCR3-CCR6+）细胞进行免疫磁珠分选

产品优势

  • 操作简单、快捷
  • 纯度高达98%
  • 无需分离柱

产品组分包括

  • EasySep™ 人Th17细胞富集试剂盒 II（产品号 #17862）
    • EasySep™ 人CCR6正选抗体混合物 II，5 mL
    • EasySep™ 人CD4+CXCR3- T细胞预富集抗体混合物，1 mL
    • EasySep™ Dextran RapidSpheres™磁珠，1 mL
    • EasySep™ 解离缓冲液，2 x 1 mL
main product photo
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

使用EasySep™人Th17细胞富集试剂盒 II，可轻松从新鲜的人外周血单个核细胞（PBMCs）或白细胞单采术样本中通过免疫磁珠正选分离出高纯度且无磁珠结合的人Th17（CD4+CXCR3-CCR6+）细胞。EasySep™结合了单克隆抗体的特异性和无柱磁性系统的简便性，迄今已广泛应用于发表的研究中超过20年。

在此EasySep™正选过程中，目的细胞首先被识别CCR6的抗体四聚体复合物和可解离的磁珠标记，与常规的结合目的细胞的磁珠不同，这种磁珠具有可解离性。使用EasySep™磁极进行细胞分离后，可通过解离试剂去除经EasySep™分选出的CCR6+细胞表面结合的磁珠，随即标记非CD4+T细胞、CD45RA+细胞及CXCR3+细胞于后续进行去除。最终分选后的细胞为高纯度的产IL-17的Th17细胞，可立即用于下游应用。

该产品可替代EasySep™人Th17细胞富集试剂盒 (产品号 #18162)， 以进行更快的细胞分选。

了解更多关于免疫磁珠EasySep™技术的工作原理，或直接选用经EasySep™人Th17细胞富集试剂盒 II 分离的、符合伦理规范的即用型冻存人原代外周血Th17细胞。探索更多的优化您实验流程的产品，包括培养基、添加剂、抗体等配套试剂。

亚型
细胞分选试剂盒
 
细胞类型
T 细胞，CD4+
 
种属
人
 
样本来源
Leukapheresis，PBMC
 
筛选方法
Positive
 
应用
细胞分选
 
品牌
EasySep
 
研究领域
免疫
 

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Data Figures

Typical EasySep™ Human Th17 Cell Enrichment Profile
Typical EasySep™ Human Th17 Cell Enrichment Profile

Figure 1. Typical EasySep™ Human Th17 Cell Enrichment Profile

Starting with fresh PBMCs, the Th17 cell content (CD4+CXCR3-CCR6+) of the isolated fraction typically ranges from 96 - 98%. Following overnight stimulation with PMA-Ionomycin, 6 - 19% of the isolated cells are IL-17+ by intracellular flow cytometry. These values vary widely among donors. IFN-γ-producing cells are typically < 5% of the enriched fraction.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human Th17 Cell Enrichment Kit II
Catalog #
17862
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Th17 Cell Enrichment Kit II
Catalog #
17862
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Th17 Cell Enrichment Kit II
Catalog #
17862
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Human Th17 Cell Enrichment Kit II
Catalog #
17862
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Human Th17 Cell Enrichment Kit II
Catalog #
17862
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
EasySep™ Human Th17 Cell Enrichment Kit II
Catalog #
17862
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Product Name
EasySep™ Human Th17 Cell Enrichment Kit II
Catalog #
17862
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (7)

Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
1:23
Video
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep...
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation

Publications (1)

Th17 cell-derived miR-155-5p modulates interleukin-17 and suppressor of cytokines signaling 1 expression during the progression of systemic sclerosis. L. Han et al. Journal of clinical laboratory analysis 2022 jun

Abstract

BACKGROUND miR-155-5p is associated with autoimmune diseases. T helper 17 (Th17) cells, interleukin (IL)-17, and suppressor of cytokines signaling 1 (SOCS1) have important roles in the pathogenesis of systemic sclerosis (SSc). The purpose of this study was to explore the role of miR-155-5p in the regulation of IL-17 and SOCS1 expression in Th17 cells and the subsequent effect on SSc disease progression. METHODS Th17 cells were isolated from peripheral blood mononuclear cells of SSc patients and healthy controls (HCs). RT-qPCR and western blotting were used to examine the expression patterns of miR-155-5p, IL-17, and SOCS1. Luciferase reporter assays were performed to confirm SOCS1 as a target of miR-155-5p. RNA pull-down assays were performed to detect the interaction of IL-17 and SOCS1 with miR-155-5p. In situ hybridization was performed to analyze the co-expression pattern of miR-155-5p and IL17A in Th17 cells. RESULTS The levels of Th17 cell-derived miR-155-5p were significantly up-regulated in SSc patients compared with HCs, and its levels were negatively correlated with SOCS1 levels. Meanwhile, miR-155-5p positively regulated IL-17 expression levels in Th17 cells isolated from SSc patients as the disease progressed. Using pmirGLO vectors, SOCS1 was confirmed as a target of miR-155-5p. The binding status of IL-17 and SOCS1 to miR-155-5p was related to SSc progression. An increase in the co-localization of miR-155-5p and IL-17 was associated with greater SSc progression. CONCLUSIONS IL-17 and SOCS1 expression modulated by Th17 cell-derived miR-155-5p are critical for SSc progression, which may provide novel insights into the pathogenesis of SSc.
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更多信息

更多信息
Species Human
Sample Source Leukapheresis, PBMC
Selection Method Positive
标记抗体
  1. 抗人CD4抗体，克隆OKT4
    抗人CD4抗体，克隆OKT4

    小鼠单克隆IgG2b抗体，抗人、恒河猴、食蟹猴CD4

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