EasySep™人Naïve Pan-T细胞分选试剂盒

免疫磁珠负选未标记的Naïve Pan-T细胞

产品号 #(选择产品)

产品号 #17961_C

免疫磁珠负选未标记的Naïve Pan-T细胞

产品优势

  • 操作简单、快捷,且无需分离柱
  • 纯度高达98%
  • 分选得到的细胞不带标记

产品组分包括

  • EasySep™人Naïve Pan T细胞分选试剂盒(产品号 #17961)
    • EasySep™人Naïve Pan T细胞分选抗体混合物,1 mL
    • EasySep™人TCR Gamma/Delta去除抗体混合物,1 mL
    • EasySep™ Dextran RapidSpheres™ 50101 磁珠,2 x 1 mL
  • RoboSep™ 人Naïve Pan T细胞分选试剂盒(产品号 #17961RF)
    • EasySep™人Naïve Pan T细胞分选抗体混合物,1 mL
    • EasySep™人TCR Gamma/Delta去除抗体混合物,1 mL
    • EasySep™ Dextran RapidSpheres™ 50101 磁珠,2 x 1 mL
    • RoboSep™ 缓冲液(产品号 #20104)
    • RoboSep™过滤吸头(产品号 #20125)
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总览

使用EasySep™人Naïve Pan-T细胞分选试剂盒,通过免疫磁珠负选,可轻松高效地从新鲜或冻存的人外周血单个核细胞(PBMCs)或洗涤后的白细胞单采术样本中分离高纯度人Naïve Pan-T细胞。EasySep™技术结合单克隆抗体的特异性和无柱磁分选系统的简便性,已在发表的研究中广泛应用超过20年。

在该EasySep™负选流程中,非目标细胞被抗体复合物与磁珠标记。表达以下标记物的非目标细胞将被去除:CD14、CD16、CD19、CD20、CD25、CD36、CD45RO、CD56、CD61、CD66b、CD94、CD123、GlyA、HLA-DR、CD57及IgM。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的细胞分离,接着简单地将目的细胞倾倒或吸取至一个新的试管中。仅需14分钟磁珠分选后,即可获得适用于流式细胞术、细胞培养或DNA/RNA提取等下游应用的原始泛T细胞。磁珠分选最快仅需14分钟,目标Naïve Pan-T即可用于流式细胞术、细胞培养或DNA/RNA提取等下游应用。

了解更多关于免疫磁珠EasySep™技术的工作原理,或如何通过RoboSep™实现免疫磁珠细胞分选全自动化。亦可选择即用型、符合伦理来源的EasySep™人Naïve Pan-T细胞试剂盒分选的人外周血Naïve Pan-T细胞冻存产品。探索更多为您实验流程优化的其它产品,包括培养基、补充剂、抗体等。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
T 细胞
 
种属

 
样本来源
Leukapheresis,PBMC
 
筛选方法
Negative
 
应用
细胞分选
 
品牌
EasySep,RoboSep
 
研究领域
免疫
 

Data Figures

Typical EasySep™ Human Naïve Pan T Cell Isolation Profile

Figure 1. Typical EasySep™ Human Naïve Pan T Cell Isolation Profile

Starting with fresh PBMCs, the naïve pan T cell content (CD3+CD45RA+CD45RO-CD197+) of the isolated fraction is typically 96.1 ± 2.3% (mean ± SD) using the purple EasySep™ Magnet. In the above example, the purities of the start and final isolated fractions are 4.5% and 97%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
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Catalog #
17961RF
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All
Language
English
Catalog #
17961
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
17961RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
17961RF
Lot #
All
Language
English
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Safety Data Sheet 3
Catalog #
17961RF
Lot #
All
Language
English
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Safety Data Sheet 4
Catalog #
17961RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
17961
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
17961
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
17961
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (8)

Publications (1)

LMBR1L regulates lymphopoiesis through Wnt/beta-catenin signaling. J. H. Choi et al. Science (New York, N.Y.) 2019

Abstract

Precise control of Wnt signaling is necessary for immune system development. In this study, we detected severely impaired development of all lymphoid lineages in mice, resulting from an N-ethyl-N-nitrosourea-induced mutation in the limb region 1-like gene (Lmbr1l), which encodes a membrane-spanning protein with no previously described function in immunity. The interaction of LMBR1L with glycoprotein 78 (GP78) and ubiquitin-associated domain-containing protein 2 (UBAC2) attenuated Wnt signaling in lymphocytes by preventing the maturation of FZD6 and LRP6 through ubiquitination within the endoplasmic reticulum and by stabilizing destruction complex" proteins. LMBR1L-deficient T cells exhibited hallmarks of Wnt/beta-catenin activation and underwent apoptotic cell death in response to proliferative stimuli. LMBR1L has an essential function during lymphopoiesis and lymphoid activation acting as a negative regulator of the Wnt/beta-catenin pathway."

更多信息

更多信息
Species Human
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
Sample Source Leukapheresis, PBMC
Selection Method Negative
标记抗体
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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