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抗人CD45RO抗体，克隆UCHL1

小鼠抗人、黑猩猩、普通狨猴CD45RO单克隆IgG2a抗体
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产品号 #60097_C

小鼠抗人、黑猩猩、普通狨猴CD45RO单克隆IgG2a抗体

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概述

UCHL1抗体与CD45RO上的细胞外表位反应，CD45RO是CD45的最短亚型，CD45是一种I型跨膜糖蛋白，是蛋白酪氨酸磷酸酶家族（受体类1/6亚家族）的成员。编码CD45细胞外RA， RB和RC多肽的外显子4,5和6的选择性剪接产生多达8个分子质量为180 - 240 kDa的异构体。切除所有三个外显子产生约180 kDa的CD45RO亚型，该亚型在活化和记忆（但不naïve） T细胞、一些B细胞亚群、活化单核细胞和巨噬细胞以及粒细胞上表达。CD45RO增强T细胞受体和B细胞受体介导的激活，是B细胞上CD22的已知配体。UCHL1抗体已被用于识别T细胞淋巴瘤和白血病，通常与抗CD45RA抗体联合使用来识别记忆和naïve T细胞。CD45RO+（记忆）T细胞的比例通常随着年龄的增长而增加。用神经氨酸酶或o -糖苷酶治疗可破坏UCHL1表位。

Subtype
Primary Antibodies
 
Target Antigen
CD45RO
 
Alternative Names
B220, CD45, GP180, LCA, L-CA, LY5, Protein tyrosine phosphatase receptor type C, PTPRC, T200
 
Reactive Species
Chimpanzee, Common Marmoset, Human
 
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, PerCP-Cyanine5.5, Unconjugated
 
Host Species
Mouse
 
Cell Type
Leukocytes, T Cells, T Cells, CD4+, T Cells, CD8+
 
Species
Human, Non-Human Primate
 
Application
Cell Isolation, ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Microscopy, Western Blotting
 
Area of Interest
Immunology, Stem Cell Biology
 
Clone
UCHL1
 
Gene ID
5788
 
Isotype
IgG2a, kappa
 

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Data Figures

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, followed by a rat anti-mouse IgG2a antibody, PE (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173 (Catalog #60071), followed by a rat anti-mouse IgG2a antibody, PE (solid line histogram). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, followed by a rat anti­mouse IgG2a antibody, PE and an anti-human CD45RA antibody, FITC. (C) Flow cytometry analysis of human PBMCs (gated on lymphocytes) and labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC­173, followed by a rat anti-mouse IgG2a antibody, PE and an anti-human CD45RA antibody, FITC.

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on CD3+ cells) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, Alexa Fluor® 488 and an anti-human CD45RA antibody, APC. (B) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Alexa Fluor® 488 (Catalog #60071AD) and an anti-human CD45RA antibody, APC.

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMSs) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, APC (filled histogram) or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, APC (Catalog #60071AZ) (solid line histogram) (B) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, APC and an anti-human CD45RA antibody, FITC. (C) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, APC and an anti-human CD45RA antibody, FITC.

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on CD3+ cells) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, Biotin, followed by streptavidin (SAV) APC and an anti-human CD45RA antibody, FITC (B) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin (Catalog #60071BT), followed by SAV APC and an anti-human CD45RA antibody, FITC.

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on CD3+ cells) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, FITC and an anti-human CD45RA antibody, APC. (B) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, FITC (Catalog #60071FI) and an anti-human CD45RA antibody, APC.

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, PE (filled histogram) or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE (Catalog #60071PE) (solid line histogram). (B) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, PE and an anti-human CD45RA antibody, APC. (C) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE and an anti-human CD45RA antibody, APC.

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, PerCP-Cy5.5 and an anti-human CD45RA antibody, FITC. Cells were gated on CD3+ cells (T lymphocytes).
(B) Flow cytometry analysis of human PBMCs labeled with a mouse IgG2a, kappa isotype control antibody, PerCP-Cy5.5 and an anti-human CD45RA antibody, FITC. Cells were gated on CD3+ cells (T lymphocytes).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1, APC
Catalog #
60097AZ
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1, Biotin
Catalog #
60097BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1, FITC
Catalog #
60097FI
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1, PE
Catalog #
60097PE, 60097PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1, PerCP-Cy5.5
Catalog #
60097PS, 60097PS.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1
Catalog #
60097
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1, APC
Catalog #
60097AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1, Biotin
Catalog #
60097BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1, FITC
Catalog #
60097FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1, PE
Catalog #
60097PE, 60097PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1, PerCP-Cy5.5
Catalog #
60097PS, 60097PS.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1
Catalog #
60097
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (3)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

更多信息

更多信息
Species Human, Non-Human Primate
Clone UCHL1
Gene Id 5788
Alternative Names B220, CD45, GP180, LCA, L-CA, LY5, Protein tyrosine phosphatase receptor type C, PTPRC, T200
Isotype IgG2a, kappa
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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