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EasySep™人脐带血CD34正选试剂盒III

免疫磁珠正选试剂盒
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产品号 #（选择产品）

产品号 #17897_C

免疫磁珠正选试剂盒

产品优势

  • 操作简单、快捷
  • 纯度可高达96%
  • 无需分离柱
  • 可与SepMate™联合使用，实现一致的高通量样本处理

产品组分包括

  • EasySep™人脐带血CD34+正选试剂盒III (#17897)
    • RosetteSep™人祖细胞基础预富集抗体混合物，2x2.5mL
    • EasySep™人CD34正选抗体混合物，2x1mL
    • EasySep™ Dextran RapidSpheres™ 50100 磁珠，2x1mL
    • RoboSep™人脐带血CD34正选试剂盒III  RosetteSep™人祖细胞基础预富集抗体混合物，2x2.5mL
    • EasySep™人CD34正选抗体混合物，2x1mL
    • EasySep™ Dextran RapidSpheres™ 50100 磁珠，2x1mL
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™过滤吸头（产品号#20125）
main product photo
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

使用简单的两步法从新鲜的全脐带血中分离高纯度的CD34+细胞。首先，使用RosetteSep™人祖细胞基础预富集抗体混合物 (产品号 #15226C)  识别T细胞、B细胞和髓系细胞表面标志物的抗体，预富集造血祖细胞。然后使用EasySep™人CD34正选抗体混合物 (产品号 #18096C) 识别CD34的抗体来分选CD34+细胞。

本试剂盒不会去除血小板。若需从可能含有血小板污染的     脐带血中分离CD34+细胞，推荐使用EasySep™人类脐带血CD34阳性分选试剂盒II（目录号17896）。

从新鲜的人外周血或白膜 分离CD34+细胞，推荐使用人全血CD34+细胞全套试剂盒（产品号 #15086）。

如需从其他样本分离CD34+细胞，包括新鲜或冻存的动员外周血、骨髓单个核细胞、冻存的脐带血单个核细胞，请使用EasySep™人CD34正选试剂盒II（产品号 #17856）。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™ (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
造血干/祖细胞
 
种属
人
 
样本来源
Cord Blood
 
筛选方法
Positive
 
应用
细胞分选
 
品牌
EasySep，RoboSep，RosetteSep
 
研究领域
免疫，干细胞生物学
 

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Data Figures

Figure 1. Typical EasySep™ Human Cord Blood CD34 Positive Selection III Isolation Profile

Starting with fresh cord blood, the CD34+ cell content of the isolated fraction is typically 87 ± 12% (mean ± SD, n=10, data obtained using the purple EasySep™ Magnet). The CD34+ cell content of the starting sample is typically 0.5 ± 0.25% (mean ± SD).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ Human Cord Blood CD34 Positive Selection Kit III
Catalog #
17897RF
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ Human Cord Blood CD34 Positive Selection Kit III
Catalog #
17897
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human Cord Blood CD34 Positive Selection Kit III
Catalog #
17897RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human Cord Blood CD34 Positive Selection Kit III
Catalog #
17897RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human Cord Blood CD34 Positive Selection Kit III
Catalog #
17897RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ Human Cord Blood CD34 Positive Selection Kit III
Catalog #
17897RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Cord Blood CD34 Positive Selection Kit III
Catalog #
17897
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Cord Blood CD34 Positive Selection Kit III
Catalog #
17897
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Human Cord Blood CD34 Positive Selection Kit III
Catalog #
17897
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (9)

Hematopoietic Stem and Progenitor Cells - Products for Your Research
Brochure
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Human CD34+ Cell Isolation Product Selection
Brochure
Human CD34+ Cell Isolation Product Selection
EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Isolation of CD34+ Cells from Human Cord Blood
Technical Bulletin
Isolation of CD34+ Cells from Human Cord Blood
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep...
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
0:57
Video
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet

Frequently Asked Questions

What is RosetteSep™?

RosetteSep™ is a rapid cell separation procedure for the isolation of purified cells directly from whole blood, without columns or magnets.

How does RosetteSep™ work?

The antibody cocktail crosslinks unwanted cells to red blood cells (RBCs), forming rosettes. The unwanted cells then pellet with the free RBCs when centrifuged over a density centrifugation medium (e.g. Ficoll-Paque™ PLUS, Lymphoprep™).

What factors affect cell recovery?

The temperature of the reagents can affect cell recovery. All reagents should be at room temperature (sample, density centrifugation medium, PBS, centrifuge) before performing the isolations. Layering can also affect recovery so be sure to carefully layer the sample to avoid mixing with the density centrifugation medium as much as possible. Be sure to collect the entire enriched culture without disturbing the RBC pellet. A small amount of density centrifugation medium can be collected without worry.

Which cell samples can RosetteSep™ be used with?

RosetteSep™ can be used with leukapheresis samples, bone marrow or buffy coat, as long as: the concentration of cells does not exceed 5 x 107 per mL (can dilute if necessary); and there are at least 100 RBCs for every nucleated cell (RBCs can be added if necessary).

Can RosetteSep™ be used with previously frozen or cultured cells?

Yes. Cells should be re-suspended at 2 - 5 x 107 cells / mL in PBS + 2% FBS. Fresh whole blood should be added at 250 µL per mL of sample, as a source of red cells.

Can RosetteSep™ be used to enrich progenitors from cord blood?

Yes. Sometimes cord blood contains immature nucleated red cells that have a lower density than mature RBCs. These immature red cells do not pellet over Ficoll™, which can lead to a higher RBC contamination than peripheral blood separations.

Does RosetteSep™ work with mouse cells?

No, but we have developed EasySep™, a magnetic-based cell isolation system which works with mouse and other non-human species.

Which anticoagulant should be used with RosetteSep™?

Peripheral blood should be collected in heparinized Vacutainers. Cord blood should be collected in ACD.

Should the anticoagulant be washed off before using RosetteSep™?

No, the antibody cocktail can be added directly to the sample.

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

更多信息

更多信息
Species Human
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™ (Catalog #21000)
Sample Source Cord Blood
Selection Method Positive
标记抗体
  1. 抗人CD34抗体，克隆8G12
    抗人CD34抗体，克隆8G12

    小鼠单克隆IgG1抗体，抗人CD34

  2. 抗人CD45抗体，克隆HI30
    抗人CD45抗体，克隆HI30

    小鼠单克隆IgG1抗体，抗人、黑猩猩CD45

  3. 抗人CD34抗体，克隆581
    抗人CD34抗体，克隆581

    小鼠单克隆IgG1抗体，抗人CD34

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