产品号 #19664_C
全血免疫磁珠负选试剂盒
全血免疫磁珠负选试剂盒
本试剂盒通过免疫磁珠负选直接从全血中分离高纯度的B-CLL细胞。该试剂盒中的去除抗体混合物不含抗CD43抗体,CD43在慢性淋巴细胞白血病 (chronic lymphocytic leukemia, CLL) 样本中的恶性细胞上表达。
该细胞分选试剂盒通过使用识别特异性细胞表面标志物的抗体来去除非B细胞。非目的细胞用抗体和EasySep™ Direct RapidSpheres™磁珠标记,使用EasySep™磁极进行分选。
轻松地将目的细胞收集到新试管中,分选后的细胞可立即用于下游应用,例如流式细胞术术、培养或DNA/RNA 提取。
磁体兼容性
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
亚型
细胞分选试剂盒
细胞类型
B 细胞
种属
人
样本来源
Whole Blood
筛选方法
Negative
应用
细胞分选
品牌
EasySep
研究领域
癌症,免疫
Figure 1. Typical EasySep™ Direct Human B-CLL Cell Isolation Profile
Starting with human whole blood from normal healthy donors, the typical B cell (CD3-CD19+) content of the non-lysed final isolated fraction is 87.0 ± 7.6% (gated on CD45) or 84.1 ± 11.7% (not gated on CD45). In the example above, the B cell (CD3-CD19+) content of the lysed whole blood start and non-lysed final isolated fraction is 4.3% and 88.0% (gated on CD45), respectively, or 4.3% and 86.2% (not gated on CD45), respectively. The starting frequency of B cells in the non-lysed whole blood start sample above is 0.004% (data not shown). The purity of isolated cells is typically higher when processing samples that have an elevated starting frequency of B cells (e.g. CLL samples).
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Thank you for your interest in IntestiCult™ Organoid Growth Medium (Human). Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
Species | Human |
---|---|
Magnet Compatibility | • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • EasyEights™ EasySep™ Magnet (Catalog #18103) |
Sample Source | Whole Blood |
Selection Method | Negative |
全血免疫磁珠负选试剂盒
小鼠单克隆IgG2b抗体,抗人、恒河猴、食蟹猴CD20
小鼠单克隆IgG1抗体,抗人、恒河猴、食蟹猴CD5
小鼠单克隆IgG1抗体,抗人、黑猩猩CD45
抗人、黑猩猩CD19的小鼠单克隆IgG1抗体
扫描二维码或搜索微信号STEMCELLTech,即可关注我们的微信平台,第一时间接收丰富的技术资源和最新的活动信息。
如您有任何问题,欢迎发消息给STEMCELLTech微信公众平台,或与我们通过电话/邮件联系:400 885 9050 INFO.CN@STEMCELL.COM。