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EasySep™ Direct人B-CLL细胞分选试剂 盒

全血免疫磁珠负选试剂盒

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产品号 #19664

直接从全血中免疫磁珠分选B-CLL细胞

产品优势

  • 99.9%红细胞去除率，无需密度梯度离心、沉降或裂解
    • 操作简单、快捷，且无需分离柱
    • 分选得到的细胞不带标记

产品组分包括

  • EasySep™ Direct人B-CLL细胞分选试剂盒 (产品号 #19664)
    • EasySep™ Direct人B-CLL细胞分选抗体混合物， 2 x 2.5 mL
    • EasySep™ Direct RapidSpheres™磁珠，4 x 2.5 mL
main product photo
EasySep™ Direct人B-CLL细胞分选试剂 盒
概述
实验数据
产品说明书及文档
应用领域
相关材料与文献

概述

本试剂盒通过免疫磁珠负选直接从全血中分离高纯度的B-CLL细胞。该试剂盒中的去除抗体混合物不含抗CD43抗体，CD43在慢性淋巴细胞白血病 (chronic lymphocytic leukemia, CLL) 样本中的恶性细胞上表达。
该细胞分选试剂盒通过使用识别特异性细胞表面标志物的抗体来去除非B细胞。非目的细胞用抗体和EasySep™ Direct RapidSpheres™磁珠标记，使用EasySep™磁极进行分选。
轻松地将目的细胞收集到新试管中，分选后的细胞可立即用于下游应用，例如流式细胞术术、培养或DNA/RNA 提取。

MAGNET COMPATIBILITY
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
 
SUBTYPE
Cell Isolation Kits
 
CELL TYPE
B Cells
 
SPECIES
Human
 
SAMPLE SOURCE
Whole Blood
 
SELECTION METHOD
Negative
 
APPLICATION
Cell Isolation
 
BRAND
EasySep
 
AREA OF INTEREST
Cancer, Immunology

实验数据

Starting with human whole blood from normal healthy donors, the typical B cell (CD3-CD19+) content of the non-lysed final isolated fraction is 87.0 ± 7.6% (gated on CD45) or 84.1 ± 11.7% (not gated on CD45)

Figure 1. Typical EasySep™ Direct Human B-CLL Cell Isolation Profile

Starting with human whole blood from normal healthy donors, the typical B cell (CD3-CD19+) content of the non-lysed final isolated fraction is 87.0 ± 7.6% (gated on CD45) or 84.1 ± 11.7% (not gated on CD45). In the example above, the B cell (CD3-CD19+) content of the lysed whole blood start and non-lysed final isolated fraction is 4.3% and 88.0% (gated on CD45), respectively, or 4.3% and 86.2% (not gated on CD45), respectively. The starting frequency of B cells in the non-lysed whole blood start sample above is 0.004% (data not shown). The purity of isolated cells is typically higher when processing samples that have an elevated starting frequency of B cells (e.g. CLL samples).

产品说明书及文档

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ Direct Human B-CLL Cell Isolation Kit
Catalog #
19664
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Direct Human B-CLL Cell Isolation Kit
Catalog #
19664
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Direct Human B-CLL Cell Isolation Kit
Catalog #
19664
Lot #
All
Language
English

应用领域

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

相关材料与文献

Educational Materials (15)

Human B Cell Isolation Product Selection
Brochure
Human B Cell Isolation Product Selection
EasySep™ Direct: Cell Isolation from Whole Blood in 20 Minutes
Brochure
EasySep™ Direct: Cell Isolation from Whole Blood in 20 Minutes
EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Tools for Cancer Research
Brochure
Tools for Cancer Research
Enhance Sensitivity of Multiple Myeloma Testing with Purified CD138+ Plasma Cells
Technical Bulletin
Enhance Sensitivity of Multiple Myeloma Testing with Purified CD138+ Plasma Cells
B Cell Isolation for More Sensitive Array-Based CLL Assays
Technical Bulletin
B Cell Isolation for More Sensitive Array-Based CLL Assays
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Antigen Processing and Presentation
Wallchart
Antigen Processing and Presentation
How to Isolate Cells Directly from Whole Blood - EasySep™ Direct Protocol, EasyEights Magnet
6:13
Video
How to Isolate Cells Directly from Whole Blood - EasySep™ Direct Protocol, EasyEights Magnet
How to Isolate Cells Directly from Whole Blood Using the EasySep™ Purple/Silver Magnets
4:30
Video
How to Isolate Cells Directly from Whole Blood Using the EasySep™ Purple/Silver Magnets
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySep™ Direct
1:44
Video
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySep™ Direct
How to Isolate Cells Directly from Whole Blood Using the Easy 50 EasySep™ Magnet: 30-Minute Protocol
5:17
Video
How to Isolate Cells Directly from Whole Blood Using the Easy 50 EasySep™ Magnet: 30-Minute Protoc...
Immunomagnetic Cell Isolation of Untouched Lymphocyte Populations Directly from Whole Blood
Scientific Poster
Immunomagnetic Cell Isolation of Untouched Lymphocyte Populations Directly from Whole Blood

Frequently Asked Questions

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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